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Developing an optimal synbiotic mix using multiple probiotics and prebiotics

Balusupati, Praveen (2012) MOBT18 20111
Degree Projects in Molecular Biology
Abstract
Abstract:

The human gastro intestinal tract (GIT) plays a significant role in the well-being and has a significant impact on human health. Probiotics are the live microbial food supplement which alters the microbial composition in gut and prebiotics are the substrates that enhance the growth of probiotics. Our interest was to examine the prebiotic utilization of this probiotics and to check the compatibility between different strains.
Screening of potential probiotic Lactobacillus and Bifidobacteria was done by culture methods and RNA analysis. Robust probiotics strains were further studied for their prebiotic utilization and compatibility between strains was studied by co-culturing them with different prebiotic compositions.
Overall... (More)
Abstract:

The human gastro intestinal tract (GIT) plays a significant role in the well-being and has a significant impact on human health. Probiotics are the live microbial food supplement which alters the microbial composition in gut and prebiotics are the substrates that enhance the growth of probiotics. Our interest was to examine the prebiotic utilization of this probiotics and to check the compatibility between different strains.
Screening of potential probiotic Lactobacillus and Bifidobacteria was done by culture methods and RNA analysis. Robust probiotics strains were further studied for their prebiotic utilization and compatibility between strains was studied by co-culturing them with different prebiotic compositions.
Overall results showed that Lactobacillus strains are more robust strains when compared to Bifidobacteria but Bifidobacteria are good prebiotic degrader when compared to Lactobacillus. No bacteriocins are produced when co-cultured with different strains indicate that this bacterial strains are compatible with each other. Starch metabolizing B. breve stimulated the growth of starch non-metabolizing L. paracasei F8, suggesting cross-feeding of L. paracasei F8 with the degraded metabolites of starch produced during starch fermentation. It is the first study where compatibility of combination of strains on combination on prebiotics is being studied

Popular science summary:

Does prebiotics stimulates the growth of probiotics?
The human gastro intestinal tract (GIT) plays a significant role in the well-being and has a significant impact on human health. The composition of the human gut microflora depends upon factors such as age of the individual, gastric pH, diet and immunological status of the host. But in recent years it has changed towards food additives with impact on the gut microbiota composition.
Probiotics are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host by improving its intestinal microbial balance .More recently, a prebiotic i.e. are defined as substrates for probiotic strains, permitting enumeration of these strains but not of pathogenic bacteria and combination of both probiotics and prebiotics, known as synbiotics have gained great interest.
Our interest was to develop and optimal synbiotic mix using multiple probiotics and prebiotics. Screening of potential probiotics Lactobacillus and Bifidobacteria strains was done by culture methods and RNA analysis. In vitro screening of probiotic strains for prebiotic degradation and robustness of the strains was determined by stressing them with acid (pH 2.5) and bile, permitting survival to the intestine. To mimic the invivo conditions strains were subjected to acid stress followed by bile stress.Co-culture of a starch metabolizing strain of B. breve and a non-starch metabolizing L. paracasei F8 was performed to elucidate a possible cross-feeding between the two different bacteria.
All tested Lactobacillus strains except L.crispatus survived well in both acidic and bile conditions. B. lactis 88 and B. lactis 10602 were most robust strains in bifidobacteria tested. Lactobacilli were not good prebiotic degrader compared to bifidobacteria. Co-culture of 2 lactobacilli (L. plantarum and L. paracasei) and 2 bifidobacteria (B. breve and B. lactis) showed a synbiotic effect.
Overall the results indicate that Lactobacilli strains were the most robust strains compared to Bifidobacteria. Bifidobacteria showed a very good metabolism of prebiotics than Lactobacillus. L. plantarum F44, L. paracasei F8, B. breve 46 and B. lactis 88 were compatible with each other, and exerted synergistic effect when grown in different combinations of prebiotics. Starch metabolizing B. breve stimulated the growth of starch non-metabolizing L. paracasei F8, suggesting cross-feeding of L. paracasei F8 with the degraded metabolites of starch produced during starch fermentation. It is the first study where compatibility of combination of strains on combination on prebiotics is being studied

Advisors: Asa Ljungh, Kanthi Kiran Kondepudi
Master´s Degree Project in Molecular biology, Microbiology 60 credits, 2013
Department of Biology, Lund University (Less)
Please use this url to cite or link to this publication:
author
Balusupati, Praveen
supervisor
organization
course
MOBT18 20111
year
type
H2 - Master's Degree (Two Years)
subject
language
English
id
3731540
date added to LUP
2013-04-26 11:19:11
date last changed
2013-04-26 11:19:11
@misc{3731540,
  abstract     = {Abstract:

The human gastro intestinal tract (GIT) plays a significant role in the well-being and has a significant impact on human health. Probiotics are the live microbial food supplement which alters the microbial composition in gut and prebiotics are the substrates that enhance the growth of probiotics. Our interest was to examine the prebiotic utilization of this probiotics and to check the compatibility between different strains.
Screening of potential probiotic Lactobacillus and Bifidobacteria was done by culture methods and RNA analysis. Robust probiotics strains were further studied for their prebiotic utilization and compatibility between strains was studied by co-culturing them with different prebiotic compositions.
Overall results showed that Lactobacillus strains are more robust strains when compared to Bifidobacteria but Bifidobacteria are good prebiotic degrader when compared to Lactobacillus. No bacteriocins are produced when co-cultured with different strains indicate that this bacterial strains are compatible with each other. Starch metabolizing B. breve stimulated the growth of starch non-metabolizing L. paracasei F8, suggesting cross-feeding of L. paracasei F8 with the degraded metabolites of starch produced during starch fermentation. It is the first study where compatibility of combination of strains on combination on prebiotics is being studied

Popular science summary:

Does prebiotics stimulates the growth of probiotics?
The human gastro intestinal tract (GIT) plays a significant role in the well-being and has a significant impact on human health. The composition of the human gut microflora depends upon factors such as age of the individual, gastric pH, diet and immunological status of the host. But in recent years it has changed towards food additives with impact on the gut microbiota composition. 
Probiotics are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host by improving its intestinal microbial balance .More recently, a prebiotic i.e. are defined as substrates for probiotic strains, permitting enumeration of these strains but not of pathogenic bacteria and combination of both probiotics and prebiotics, known as synbiotics have gained great interest.
Our interest was to develop and optimal synbiotic mix using multiple probiotics and prebiotics. Screening of potential probiotics Lactobacillus and Bifidobacteria strains was done by culture methods and RNA analysis. In vitro screening of probiotic strains for prebiotic degradation and robustness of the strains was determined by stressing them with acid (pH 2.5) and bile, permitting survival to the intestine. To mimic the invivo conditions strains were subjected to acid stress followed by bile stress.Co-culture of a starch metabolizing strain of B. breve and a non-starch metabolizing L. paracasei F8 was performed to elucidate a possible cross-feeding between the two different bacteria.
All tested Lactobacillus strains except L.crispatus survived well in both acidic and bile conditions. B. lactis 88 and B. lactis 10602 were most robust strains in bifidobacteria tested. Lactobacilli were not good prebiotic degrader compared to bifidobacteria. Co-culture of 2 lactobacilli (L. plantarum and L. paracasei) and 2 bifidobacteria (B. breve and B. lactis) showed a synbiotic effect.
Overall the results indicate that Lactobacilli strains were the most robust strains compared to Bifidobacteria. Bifidobacteria showed a very good metabolism of prebiotics than Lactobacillus. L. plantarum F44, L. paracasei F8, B. breve 46 and B. lactis 88 were compatible with each other, and exerted synergistic effect when grown in different combinations of prebiotics. Starch metabolizing B. breve stimulated the growth of starch non-metabolizing L. paracasei F8, suggesting cross-feeding of L. paracasei F8 with the degraded metabolites of starch produced during starch fermentation. It is the first study where compatibility of combination of strains on combination on prebiotics is being studied

Advisors: Asa Ljungh, Kanthi Kiran Kondepudi
Master´s Degree Project in Molecular biology, Microbiology 60 credits, 2013
Department of Biology, Lund University},
  author       = {Balusupati, Praveen},
  language     = {eng},
  note         = {Student Paper},
  title        = {Developing an optimal synbiotic mix using multiple probiotics and prebiotics},
  year         = {2012},
}