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The effect of standard heart preservation on the endothelial and smooth muscle function of the coronary artery

Al Siyabi, Sultan (2013) MOBT25 20122
Degree Projects in Molecular Biology
Abstract
Abstract:

Background. In clinical heart transplantation, the most common method used to preserve the donor heart is cold flush perfusion with a cardioplegic solution, e.g. St. Thomas Solution. This method gives safe preservation for up to 4 hours.
Methods. In organ baths, vessels segments of porcine coronary arteries were used to study the smooth muscle contractility and endothelial dependent and independent relaxation in fresh vessel segments, and after preservation with St. Thomas Solution followed by storage in cold Kreb’s solution for 4 and 24 hours
Results. There was no significant difference in the contraction capacity induced by either high potassium Kreb’s solution or the thromboxane A2 analogue U46619 after 4 hours and 24... (More)
Abstract:

Background. In clinical heart transplantation, the most common method used to preserve the donor heart is cold flush perfusion with a cardioplegic solution, e.g. St. Thomas Solution. This method gives safe preservation for up to 4 hours.
Methods. In organ baths, vessels segments of porcine coronary arteries were used to study the smooth muscle contractility and endothelial dependent and independent relaxation in fresh vessel segments, and after preservation with St. Thomas Solution followed by storage in cold Kreb’s solution for 4 and 24 hours
Results. There was no significant difference in the contraction capacity induced by either high potassium Kreb’s solution or the thromboxane A2 analogue U46619 after 4 hours and 24 hours of preservation compared to a fresh control group. The endothelial dependent relaxation, induced by Substance P, was significantly impaired after 4 hours compared to the control group (p<0.05). Extending the preservation time from 4 to 24 hours worsened the impairment in endothelial dependent relaxation (p<0.001). The endothelium independent relaxation induced by papaverine, was also significantly reduced after 4 and 24 hours (p<0.05).
Conclusion. The commonly used method of cold cardioplegic preservation of donor hearts had adverse effects on endothelial-dependent and independent relaxation of coronary arteries whereas the contraction capacity was intact after both 4 and 24 hours preservation. Taken together these results indicate an increased tendency of vasoconstriction in the coronary arteries of hearts preserved with cold ischemic storage for 4 hours or more. (Less)
Please use this url to cite or link to this publication:
author
Al Siyabi, Sultan
supervisor
organization
course
MOBT25 20122
year
type
H2 - Master's Degree (Two Years)
subject
language
English
id
4054022
date added to LUP
2013-09-19 12:13:36
date last changed
2013-09-19 12:13:36
@misc{4054022,
  abstract     = {Abstract:

Background. In clinical heart transplantation, the most common method used to preserve the donor heart is cold flush perfusion with a cardioplegic solution, e.g. St. Thomas Solution. This method gives safe preservation for up to 4 hours. 
Methods. In organ baths, vessels segments of porcine coronary arteries were used to study the smooth muscle contractility and endothelial dependent and independent relaxation in fresh vessel segments, and after preservation with St. Thomas Solution followed by storage in cold Kreb’s solution for 4 and 24 hours 
Results. There was no significant difference in the contraction capacity induced by either high potassium Kreb’s solution or the thromboxane A2 analogue U46619 after 4 hours and 24 hours of preservation compared to a fresh control group. The endothelial dependent relaxation, induced by Substance P, was significantly impaired after 4 hours compared to the control group (p<0.05). Extending the preservation time from 4 to 24 hours worsened the impairment in endothelial dependent relaxation (p<0.001). The endothelium independent relaxation induced by papaverine, was also significantly reduced after 4 and 24 hours (p<0.05). 
Conclusion. The commonly used method of cold cardioplegic preservation of donor hearts had adverse effects on endothelial-dependent and independent relaxation of coronary arteries whereas the contraction capacity was intact after both 4 and 24 hours preservation. Taken together these results indicate an increased tendency of vasoconstriction in the coronary arteries of hearts preserved with cold ischemic storage for 4 hours or more.},
  author       = {Al Siyabi, Sultan},
  language     = {eng},
  note         = {Student Paper},
  title        = {The effect of standard heart preservation on the endothelial and smooth muscle function of the coronary artery},
  year         = {2013},
}