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Genotypic variation in sugar beet (Beta vulgaris L.) affects growth-stimulation induced by Trichoderma harzianum T-22

Schmidt, John (2013) MOBN16 20131
Degree Projects in Molecular Biology
Abstract
Abstract

Fungi of the genus Trichoderma are commonly used as biocontrol agents in modern agriculture because of their beneficial effects on both plant growth and pest and pathogen control. The mechanism behind the biocontrol abilities of Trichoderma spp. has been shown to consist of direct pathogen control but also indirect by altering of the plant genetic expression, inducing changes in plant behaviour and growth. The ability of Trichoderma spp. to affect the genetic expression of the plant suggests that the plant genetic makeup is of great importance in the response to the fungi. The aim of the present study was to investigate the growth induced effect of T. harzianum T-22 in sugar beet with the aim of assisting future sugar beet... (More)
Abstract

Fungi of the genus Trichoderma are commonly used as biocontrol agents in modern agriculture because of their beneficial effects on both plant growth and pest and pathogen control. The mechanism behind the biocontrol abilities of Trichoderma spp. has been shown to consist of direct pathogen control but also indirect by altering of the plant genetic expression, inducing changes in plant behaviour and growth. The ability of Trichoderma spp. to affect the genetic expression of the plant suggests that the plant genetic makeup is of great importance in the response to the fungi. The aim of the present study was to investigate the growth induced effect of T. harzianum T-22 in sugar beet with the aim of assisting future sugar beet breeding for improved T. harzianum T-22 competence. We present results that indicate that a genotypic variability of sugar beet affected the outcome of the interaction to the growth promoting biocontrol agent T. harzianum T-22. We also demonstrate a variation in response to T. harzianum T-22 between sugar beet cultivated in sterile and soil conditions as well as a novel assay in sugar beet seed surface sterilization. (Less)
Abstract
Popular science summary

Growth promoting effects of Trichoderma harzianum on sugar beet

Imagine if a plant could be prepared to a pathogen attack before the attack occurs, or if a plant could grow stronger, without an excessive addition of inorganic chemicals. It can, it just need a little help from a fungus.

The fungus that stimulates plants is called Trichoderma harzianum. It induces both pest resistance and growth to a variety of plant species. T. harzianum is the active ingredient in several commercial biocontrol products and is utilized as a biological complement or addition to chemical pesticides in controlling plant diseases. The mechanisms by which the fungus and plants interacts is still under investigation, but it has... (More)
Popular science summary

Growth promoting effects of Trichoderma harzianum on sugar beet

Imagine if a plant could be prepared to a pathogen attack before the attack occurs, or if a plant could grow stronger, without an excessive addition of inorganic chemicals. It can, it just need a little help from a fungus.

The fungus that stimulates plants is called Trichoderma harzianum. It induces both pest resistance and growth to a variety of plant species. T. harzianum is the active ingredient in several commercial biocontrol products and is utilized as a biological complement or addition to chemical pesticides in controlling plant diseases. The mechanisms by which the fungus and plants interacts is still under investigation, but it has become evident that they result in changes in the plants gene expression. It is therefore not unlikely that plants with different genetic makeup also respond differently to T. harzianum.

We wondered if there is a variation in T. harzianum-induced growth between different cultivars of sugar beet. In this experiment, we cultivated plants from 9 different cultivars of sugar beet in both greenhouse and sterile conditions. The sugar beet plants were treated with spores from T. harzianum and the effect on growth caused by the T. harzianum treatment was measured on both shoots and roots.

T. harzianum-induced growth varies between cultivars
We discovered that T. harzianum affected sugar beet growth differently when cultivated in greenhouse or in a sterile environment. In a sterile environment, T. harzianum inhibited growth of all the included sugar beet cultivars. In the greenhouse however, T. harzianum caused an increase in growth of some sugar beet cultivars, but not in all.

Since some of the sugar beet cultivars showed an increase in growth when treated with T. harzianum while other cultivars did not, we conclude that there is a so called genotypic variation in sugar beet that affect the growth effects induced by T. harzianum. This information may be useful in elucidating the complex interactions between plants and T. harzianum. It may also serve as a first step in selecting new breeding material to obtain sugar beet plants that utilizes the beneficial effects of T. harzianum at its full potential.


Supervisor: Allan Rasmusson
Ass. Supervisor: Susanne Widell
Master´s Degree Project in Plant Biology 45 hp, 2013
Deptartment of Biology, Lund University
In cooperation with Syngenta Seeds AB (Less)
Please use this url to cite or link to this publication:
author
Schmidt, John
supervisor
organization
course
MOBN16 20131
year
type
H2 - Master's Degree (Two Years)
subject
language
English
id
4195935
date added to LUP
2013-12-16 15:10:16
date last changed
2013-12-16 15:10:16
@misc{4195935,
  abstract     = {Popular science summary

Growth promoting effects of Trichoderma harzianum on sugar beet 

Imagine if a plant could be prepared to a pathogen attack before the attack occurs, or if a plant could grow stronger, without an excessive addition of inorganic chemicals. It can, it just need a little help from a fungus. 

The fungus that stimulates plants is called Trichoderma harzianum. It induces both pest resistance and growth to a variety of plant species. T. harzianum is the active ingredient in several commercial biocontrol products and is utilized as a biological complement or addition to chemical pesticides in controlling plant diseases. The mechanisms by which the fungus and plants interacts is still under investigation, but it has become evident that they result in changes in the plants gene expression. It is therefore not unlikely that plants with different genetic makeup also respond differently to T. harzianum. 

We wondered if there is a variation in T. harzianum-induced growth between different cultivars of sugar beet. In this experiment, we cultivated plants from 9 different cultivars of sugar beet in both greenhouse and sterile conditions. The sugar beet plants were treated with spores from T. harzianum and the effect on growth caused by the T. harzianum treatment was measured on both shoots and roots. 

T. harzianum-induced growth varies between cultivars 
We discovered that T. harzianum affected sugar beet growth differently when cultivated in greenhouse or in a sterile environment. In a sterile environment, T. harzianum inhibited growth of all the included sugar beet cultivars. In the greenhouse however, T. harzianum caused an increase in growth of some sugar beet cultivars, but not in all. 

Since some of the sugar beet cultivars showed an increase in growth when treated with T. harzianum while other cultivars did not, we conclude that there is a so called genotypic variation in sugar beet that affect the growth effects induced by T. harzianum. This information may be useful in elucidating the complex interactions between plants and T. harzianum. It may also serve as a first step in selecting new breeding material to obtain sugar beet plants that utilizes the beneficial effects of T. harzianum at its full potential. 


Supervisor: Allan Rasmusson 
Ass. Supervisor: Susanne Widell 
Master´s Degree Project in Plant Biology 45 hp, 2013 
Deptartment of Biology, Lund University 
In cooperation with Syngenta Seeds AB},
  author       = {Schmidt, John},
  language     = {eng},
  note         = {Student Paper},
  title        = {Genotypic variation in sugar beet (Beta vulgaris L.) affects growth-stimulation induced by Trichoderma harzianum T-22},
  year         = {2013},
}