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Identification and functional studies of genetic aberrations underlying adipocytic tumour development

Alemán, Cristina (2014) MOBT19 20132
Degree Projects in Molecular Biology
Abstract
Popular science summary:

Genetic aberrations underlying adipocytic tumour development

Adipocytic tumours are the most common mesenchymal tumours. We have identified genetic aberrations in such tumours using high-resolution single nucleotide polymorphism (SNP) array analysis. We have also performed functional studies of specific genetic aberrations and found genes of importance for tumour development and differentiation.

Tumours that resemble adipose tissue (fat) are known as adipocytic tumours. There are several different histological subtypes and we have investigated four benign and two malignant subtypes using SNP array analysis. In total, we investigated 38 different tumours and in line with previous reports we found copy... (More)
Popular science summary:

Genetic aberrations underlying adipocytic tumour development

Adipocytic tumours are the most common mesenchymal tumours. We have identified genetic aberrations in such tumours using high-resolution single nucleotide polymorphism (SNP) array analysis. We have also performed functional studies of specific genetic aberrations and found genes of importance for tumour development and differentiation.

Tumours that resemble adipose tissue (fat) are known as adipocytic tumours. There are several different histological subtypes and we have investigated four benign and two malignant subtypes using SNP array analysis. In total, we investigated 38 different tumours and in line with previous reports we found copy number alterations of genes such as RB1, NF1, TP53, MDM2 and HMGA2. We conclude that SNP array analysis is a good method for finding already known copy number alterations in adipocytic tumours.

However, to detect novel aberrations of importance for the development of these tumours, we believe that methods with higher resolution, such as various deep sequencing analyses, should be applied.

We have also used lentiviral-shRNA transduction to silence genes that we believe are of importance for the development of specific adipocytic tumours. The silencing was done in two different cell lines and confirms the importance of the investigated genes.

Advisor: Karolin Hansén Nord
Master´s Degree Project 60 credits in Molecular Genetics at Lund University 2013/2014
Department of Biology, Lund University (Less)
Please use this url to cite or link to this publication:
author
Alemán, Cristina
supervisor
organization
course
MOBT19 20132
year
type
H2 - Master's Degree (Two Years)
subject
language
English
id
4497656
date added to LUP
2014-06-19 15:12:34
date last changed
2014-06-19 15:12:34
@misc{4497656,
  abstract     = {Popular science summary:

Genetic aberrations underlying adipocytic tumour development 

Adipocytic tumours are the most common mesenchymal tumours. We have identified genetic aberrations in such tumours using high-resolution single nucleotide polymorphism (SNP) array analysis. We have also performed functional studies of specific genetic aberrations and found genes of importance for tumour development and differentiation. 

Tumours that resemble adipose tissue (fat) are known as adipocytic tumours. There are several different histological subtypes and we have investigated four benign and two malignant subtypes using SNP array analysis. In total, we investigated 38 different tumours and in line with previous reports we found copy number alterations of genes such as RB1, NF1, TP53, MDM2 and HMGA2. We conclude that SNP array analysis is a good method for finding already known copy number alterations in adipocytic tumours.

However, to detect novel aberrations of importance for the development of these tumours, we believe that methods with higher resolution, such as various deep sequencing analyses, should be applied. 

We have also used lentiviral-shRNA transduction to silence genes that we believe are of importance for the development of specific adipocytic tumours. The silencing was done in two different cell lines and confirms the importance of the investigated genes. 

Advisor: Karolin Hansén Nord
Master´s Degree Project 60 credits in Molecular Genetics at Lund University 2013/2014 
Department of Biology, Lund University},
  author       = {Alemán, Cristina},
  language     = {eng},
  note         = {Student Paper},
  title        = {Identification and functional studies of genetic aberrations underlying adipocytic tumour development},
  year         = {2014},
}