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Screening for anti-allergic properties of probiotic lysates - For the down-regulation of Th2-secreted cytokines in humans suffering from Allergic rhinitis

Kulic, Amra LU (2016) KBK820 20152
Pure and Applied Biochemistry
Abstract
Allergic rhinitis is a common disease affecting millions of people worldwide. Biocare Copenhagen A/S, a Danish biotechnology company decided to screen a number of probiotic lysates, with the aim to identify suitable strains with potential anti-allergic properties, that could regulate production of cytokines and divert the immune system in a regulatory or tolerant mode.

People suffering from allergic rhinitis have an over stimulated response of Th2- cytokines, leading to an imbalance in the immune system. Screening of eight strains in a joint in vitro trial was carried out in collaboration with the Danish contract lab. Bioneer, in order to identify bacteria strains that could down regulate Th2-cell immune response to potential allergens.... (More)
Allergic rhinitis is a common disease affecting millions of people worldwide. Biocare Copenhagen A/S, a Danish biotechnology company decided to screen a number of probiotic lysates, with the aim to identify suitable strains with potential anti-allergic properties, that could regulate production of cytokines and divert the immune system in a regulatory or tolerant mode.

People suffering from allergic rhinitis have an over stimulated response of Th2- cytokines, leading to an imbalance in the immune system. Screening of eight strains in a joint in vitro trial was carried out in collaboration with the Danish contract lab. Bioneer, in order to identify bacteria strains that could down regulate Th2-cell immune response to potential allergens.

The immune system, its responses, the mechanisms behind how the white blood cells interact with each other and other cells, as well as probiotic lysates (inactivated probiotic bacteria) and their mechanisms of action are two very complex areas.

Studies have shown that probiotics have the power to modulate immune responses and are thus an essential key player in immunology. Today many different probiotic strains have been discovered having different effects and capacities on human epithelial cells, resulting in beneficial effects in the host after ingestion.

In this project eight probiotic lysates were used to try and down-regulate the Th2- cytokine response in allergic individuals, investigate which tested lysates are the most effective and reconfirm these results in a second part of the experiments.

Methods used in the in vitro experimental work are Bioneers dendritic cell (DC) services involving the generation of human DCs as well as a DC-Immuneregulation model. The DCs stimulate T-cell differentiation upon treatment with both probiotic lysates and an allergy-inducing cocktail, to make the DCs mature and induce a signalling pathway, which results in Th2-cytokine secretion. These secreted cytokine levels and analysis to conclude the results mark the end-point of the experiment.


The results indicate that all eight probiotic lysates had potential to down-regulate the Th2-response, where probiotic 4 and 7 showed to be best in test. These two probiotics where therefore further used in the second part of the experiment to try and reconfirm their suppressing effects on IL-4, IL-5 and IL-13 cytokines secreted by the Th2-cells.

The overall conclusion is that the second part of the experiment was not as successful as the first. Still it was enough to reconfirm that probiotic 4 and 7 where the most efficient ones.

These probiotic lysates could in future terms be used for in vivo studies as well as clinical trials, thus mark the beginning of the possible development of a new bacteria based food supplement that could help individuals suffer from allergic rhinitis. (Less)
Popular Abstract
This master thesis project is done in cooperation with the Danish Biotechnology Company BioCare Copenhagen. The aim of the project is to screen for anti-allergic effects in eight different probiotic lysates, for the down- regulation of Th2-secreted cytokines in humans suffering from Allergic rhinitis.
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author
Kulic, Amra LU
supervisor
organization
course
KBK820 20152
year
type
H2 - Master's Degree (Two Years)
subject
keywords
probiotics, applied biochemistry, allergic, cytokine
language
English
id
8518819
date added to LUP
2016-06-17 11:33:02
date last changed
2016-06-17 11:33:02
@misc{8518819,
  abstract     = {Allergic rhinitis is a common disease affecting millions of people worldwide. Biocare Copenhagen A/S, a Danish biotechnology company decided to screen a number of probiotic lysates, with the aim to identify suitable strains with potential anti-allergic properties, that could regulate production of cytokines and divert the immune system in a regulatory or tolerant mode.

People suffering from allergic rhinitis have an over stimulated response of Th2- cytokines, leading to an imbalance in the immune system. Screening of eight strains in a joint in vitro trial was carried out in collaboration with the Danish contract lab. Bioneer, in order to identify bacteria strains that could down regulate Th2-cell immune response to potential allergens.

The immune system, its responses, the mechanisms behind how the white blood cells interact with each other and other cells, as well as probiotic lysates (inactivated probiotic bacteria) and their mechanisms of action are two very complex areas.

Studies have shown that probiotics have the power to modulate immune responses and are thus an essential key player in immunology. Today many different probiotic strains have been discovered having different effects and capacities on human epithelial cells, resulting in beneficial effects in the host after ingestion.

In this project eight probiotic lysates were used to try and down-regulate the Th2- cytokine response in allergic individuals, investigate which tested lysates are the most effective and reconfirm these results in a second part of the experiments.

Methods used in the in vitro experimental work are Bioneers dendritic cell (DC) services involving the generation of human DCs as well as a DC-Immuneregulation model. The DCs stimulate T-cell differentiation upon treatment with both probiotic lysates and an allergy-inducing cocktail, to make the DCs mature and induce a signalling pathway, which results in Th2-cytokine secretion. These secreted cytokine levels and analysis to conclude the results mark the end-point of the experiment.
 

The results indicate that all eight probiotic lysates had potential to down-regulate the Th2-response, where probiotic 4 and 7 showed to be best in test. These two probiotics where therefore further used in the second part of the experiment to try and reconfirm their suppressing effects on IL-4, IL-5 and IL-13 cytokines secreted by the Th2-cells.

The overall conclusion is that the second part of the experiment was not as successful as the first. Still it was enough to reconfirm that probiotic 4 and 7 where the most efficient ones.

These probiotic lysates could in future terms be used for in vivo studies as well as clinical trials, thus mark the beginning of the possible development of a new bacteria based food supplement that could help individuals suffer from allergic rhinitis.},
  author       = {Kulic, Amra},
  keyword      = {probiotics,applied biochemistry,allergic,cytokine},
  language     = {eng},
  note         = {Student Paper},
  title        = {Screening for anti-allergic properties of probiotic lysates - For the down-regulation of Th2-secreted cytokines in humans suffering from Allergic rhinitis},
  year         = {2016},
}