Probing Control of a Fedbatch Fermentation. Simulation and Implementation in ABB Industrial IT
(2004) In MSc ThesesDepartment of Automatic Control
- Abstract
- During the production of pharmaceutical products, a central stage is so called fermentation process i.e. cultivation of bacteria in a biological reactor. In the scope of this Master Thesis the product is recombinant protein. The goal for the fermentation process is to keep a high cell growth and a high production of recombinant proteins. For these purposes, an automatic control algorithm has been presented in (Åkesson, 1999), with some modifications described well in (Velut et al, 2002). The algorithm is designed to avoid acetate accumulation while maintaining a high glucose feed by monitoring the dissolved oxygen tension in the biological reactor. The acetate is an undesired byproduct that inhibits both cell replication and production of... (More)
- During the production of pharmaceutical products, a central stage is so called fermentation process i.e. cultivation of bacteria in a biological reactor. In the scope of this Master Thesis the product is recombinant protein. The goal for the fermentation process is to keep a high cell growth and a high production of recombinant proteins. For these purposes, an automatic control algorithm has been presented in (Åkesson, 1999), with some modifications described well in (Velut et al, 2002). The algorithm is designed to avoid acetate accumulation while maintaining a high glucose feed by monitoring the dissolved oxygen tension in the biological reactor. The acetate is an undesired byproduct that inhibits both cell replication and production of recombinant proteins. The automatic control algorithm and a process model has first been implemented in Matlab and later in Control Builder,(a developing environment for IEC 61131 Control Languages. It is part of the Industrial IT 800xA System developed by ABB and is a comprehensive process automation system. The Control Builder is mainly used to implement the controller programs while the Industrial IT 800xA System has been used to control/monitor/log different variables during simulation and later in the field test. After verifying the implementation in Control Builder by comparing the simulation in Soft controller (a simulation tool for real time simulation) with simulation in Matlab, the controller programs are ready for the final test. The final test was performed in a physical biological reactor located at division of Biotechnology at the Chemical Center of Lund Institute of Technology where the controller programs were downloaded into an ABB AC800M controller. The final test of the controller programs in Lund showed to be very successful at the first attempt. It managed to keep a high cell growth and at the same time it has prevented production of acetate. (Less)
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@misc{8848000,
abstract = {{During the production of pharmaceutical products, a central stage is so called fermentation process i.e. cultivation of bacteria in a biological reactor. In the scope of this Master Thesis the product is recombinant protein. The goal for the fermentation process is to keep a high cell growth and a high production of recombinant proteins. For these purposes, an automatic control algorithm has been presented in (Åkesson, 1999), with some modifications described well in (Velut et al, 2002). The algorithm is designed to avoid acetate accumulation while maintaining a high glucose feed by monitoring the dissolved oxygen tension in the biological reactor. The acetate is an undesired byproduct that inhibits both cell replication and production of recombinant proteins. The automatic control algorithm and a process model has first been implemented in Matlab and later in Control Builder,(a developing environment for IEC 61131 Control Languages. It is part of the Industrial IT 800xA System developed by ABB and is a comprehensive process automation system. The Control Builder is mainly used to implement the controller programs while the Industrial IT 800xA System has been used to control/monitor/log different variables during simulation and later in the field test. After verifying the implementation in Control Builder by comparing the simulation in Soft controller (a simulation tool for real time simulation) with simulation in Matlab, the controller programs are ready for the final test. The final test was performed in a physical biological reactor located at division of Biotechnology at the Chemical Center of Lund Institute of Technology where the controller programs were downloaded into an ABB AC800M controller. The final test of the controller programs in Lund showed to be very successful at the first attempt. It managed to keep a high cell growth and at the same time it has prevented production of acetate.}},
author = {{Li, Tory}},
issn = {{0280-5316}},
language = {{eng}},
note = {{Student Paper}},
series = {{MSc Theses}},
title = {{Probing Control of a Fedbatch Fermentation. Simulation and Implementation in ABB Industrial IT}},
year = {{2004}},
}