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Incomplete Oxidation of Bio-butanol To Bio-butyraldehyde Using Gluconobacter oxydans DSM 2343

Elsabaa, Hossameldeen (2017) MOBT01 20162
Degree Projects in Molecular Biology
Popular Abstract
Until now we still depend on oil and natural gas to supply us with fuels and other chemicals as a foundation to our economic advance. The global population is expected to reach 9 billion by 2050. With increasing population, the oil resource consumption will also increase, and an alternative source must be found and developed to supply us with chemicals and fuel needed for our economical advances, as oil and fossil fuels face shortage and increased consumption. In the recent years an intense and focused research was toward bio‐refinary as an alternative way to petro‐refinery so, we can obtain chemicals and building blocks from biomass instead of fossil fuels.

A large and branched polyol trimethylolpropane (TMP) is a potential candidate... (More)
Until now we still depend on oil and natural gas to supply us with fuels and other chemicals as a foundation to our economic advance. The global population is expected to reach 9 billion by 2050. With increasing population, the oil resource consumption will also increase, and an alternative source must be found and developed to supply us with chemicals and fuel needed for our economical advances, as oil and fossil fuels face shortage and increased consumption. In the recent years an intense and focused research was toward bio‐refinary as an alternative way to petro‐refinery so, we can obtain chemicals and building blocks from biomass instead of fossil fuels.

A large and branched polyol trimethylolpropane (TMP) is a potential candidate to be produced from renewable resources. TMP formation is a result of the reaction of formaldehyde and butyraldehyde. As no direct biotechnological way is developed to obtain TMP from biomass via microbial oxidation of butanol or methanol, the oxidation of alcohols to aldehydes is an important step for synthesis and transformation of molecules.

By using resting cells, different oxidative bacteria were tested in order to find a good candidate for the butanol oxidation. Bacterial strains tested for production of bio‐based chemicals are: Gluconobacter oxydans DSM 2343 and DSM 50049, Rhodococcus erythropolis DSM 43066, and Mycobacterium sp. MS1601. After the best candidate for oxidation was found a study was performed to improve the oxidation of butanol to the corresponding aldehyde and identify the responsible enzyme.

G. oxydans DSM 2343 is a promising bacterium for the oxidation of butanol to butyraldehyde with high yield and high selectivity. Furthermore, it can be used for production of many aldehydes from oxidation of different alcohols. Oxidation of 15 g/l butanol was carried out successfully in a bench scale bioreactor and resulted in 70% butyraldehyde yield, which is a great step towards industrial scale and application. Oxidation of bio‐butanol was carried out under moderate conditions in cheap medium and low byproduct formation which helps in downstream processing, lower energy consumption, and is environmentally safe. We also found that pH has a large effect in the oxidation process where pH 5 givave the highest yield of butyraldehyde. We assume that the putative alcohol/aldehyde dehydrogenase GOX2676 gene is responsible for activity according to preliminary experiments.

Advisor: Sang‐Huyn Pyo, Mahmoud Sayed
Master´s Degree Project in Molecular Biology 60 credits 2017
Department of Biology, Faculty of Science, Lund University (Less)
Please use this url to cite or link to this publication:
author
Elsabaa, Hossameldeen
supervisor
organization
course
MOBT01 20162
year
type
H2 - Master's Degree (Two Years)
subject
language
English
id
8926554
date added to LUP
2017-09-29 11:10:58
date last changed
2017-09-29 11:10:58
@misc{8926554,
  author       = {Elsabaa, Hossameldeen},
  language     = {eng},
  note         = {Student Paper},
  title        = {Incomplete Oxidation of Bio-butanol To Bio-butyraldehyde Using Gluconobacter oxydans DSM 2343},
  year         = {2017},
}