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Metastatic model of colon cancer in wild type and cysteinyl leukotriene receptor 1-/- B6 mice

Devarajan, Anuja (2017) MOBY01 20171
Degree Projects in Molecular Biology
Abstract
Background: Colorectal cancer constitutes a major malignancy and the fourth major cause of deaths associated with cancer. Chronic inflammatory bowel diseases like Crohn´s disease and ulcerative colitis are one of the risk factors for the development of CRC. Cysteinyl leukotrienes (CysLTs) regulate both acute and chronic inflammation through lipophilic signaling. Upregulation of the expression of CysLT1R in colon cancer has been associated with poor prognosis of patients. ApcMin/+ mice lacking the Cysltr1 gene had reduced tumor burden compared to wildtype mice. Aim: To investigate the role of CysLT1R in a metastatic mouse model of colon cancer. Methods: Breeding and genotyping of the wildtype mice and CysLT1R -/- mice. Culturing of the... (More)
Background: Colorectal cancer constitutes a major malignancy and the fourth major cause of deaths associated with cancer. Chronic inflammatory bowel diseases like Crohn´s disease and ulcerative colitis are one of the risk factors for the development of CRC. Cysteinyl leukotrienes (CysLTs) regulate both acute and chronic inflammation through lipophilic signaling. Upregulation of the expression of CysLT1R in colon cancer has been associated with poor prognosis of patients. ApcMin/+ mice lacking the Cysltr1 gene had reduced tumor burden compared to wildtype mice. Aim: To investigate the role of CysLT1R in a metastatic mouse model of colon cancer. Methods: Breeding and genotyping of the wildtype mice and CysLT1R -/- mice. Culturing of the MC-38 mice colon cancer cells. A pilot study with the colon cancer metastatic mice model developed. The MC38 cell line (murine colon adenocarcinoma derived) was injected into the spleen of the same inbred mouse strain. Results: The optimum number of cells to be injected was 3x105 cells for 3 weeks where visible metastasis was observed. Conclusion: A trend was observed in this model where more metastatic tumours we developed in mice with intact Cysltr1 than the mice that lacked the Cysltr1. This model would aid in analysing the role of CysLT1R in metastatic colon cancer. (Less)
Popular Abstract
Colorectal cancer, the cancer of colon is the fourth major cause of deaths associated with cancer. Westernised life style and inflammation are potential risk factors of CRC. Metastasis is the spread of cancer cells from an initial tumour site to other organs. The most common site of colon cancer metastasis is the liver followed by lungs.
Cysteinyl leukotrienes (CysLTs) are proteins involved in inducing inflammation. The over expression of one of their receptor CysLT1R results in poor response to cancer treatment. The current study used two types of mice, one with a functioning CysLT1R and the other type without a functioning CysLT1R.
This project focused on the optimization of the cells to be injected and the duration it took for the... (More)
Colorectal cancer, the cancer of colon is the fourth major cause of deaths associated with cancer. Westernised life style and inflammation are potential risk factors of CRC. Metastasis is the spread of cancer cells from an initial tumour site to other organs. The most common site of colon cancer metastasis is the liver followed by lungs.
Cysteinyl leukotrienes (CysLTs) are proteins involved in inducing inflammation. The over expression of one of their receptor CysLT1R results in poor response to cancer treatment. The current study used two types of mice, one with a functioning CysLT1R and the other type without a functioning CysLT1R.
This project focused on the optimization of the cells to be injected and the duration it took for the liver metastasis to develop. The metastatic mouse model was obtained by injecting cancer cells into the spleen of the mice. Two to three weeks after the injection of cancer cells, the mouse was sacrificed and the liver, lungs and other regions with tumours were extracted and analysed. We observed that the colon cancer cells metastasize to the liver and the mice that lacked the CysLT1R developed fewer metastasis. Blocking of this receptor using a drug could help in treatment of patients with colon cancer metastasis.

Master’s Degree Project in Molecular Biology 30 credits 2017
Department of Biology, Lund University

Advisor: Anita Sjölander
Division of Cell and Experimental Pathology, Department of Laboratory Medicine, Lund University, Skåne University Hospital, Malmö, Sweden (Less)
Please use this url to cite or link to this publication:
author
Devarajan, Anuja
supervisor
organization
course
MOBY01 20171
year
type
H1 - Master's Degree (One Year)
subject
language
English
id
8927602
date added to LUP
2017-10-19 14:01:51
date last changed
2017-10-20 10:49:42
@misc{8927602,
  abstract     = {Background: Colorectal cancer constitutes a major malignancy and the fourth major cause of deaths associated with cancer. Chronic inflammatory bowel diseases like Crohn´s disease and ulcerative colitis are one of the risk factors for the development of CRC. Cysteinyl leukotrienes (CysLTs) regulate both acute and chronic inflammation through lipophilic signaling. Upregulation of the expression of CysLT1R in colon cancer has been associated with poor prognosis of patients. ApcMin/+ mice lacking the Cysltr1 gene had reduced tumor burden compared to wildtype mice. Aim: To investigate the role of CysLT1R in a metastatic mouse model of colon cancer. Methods: Breeding and genotyping of the wildtype mice and CysLT1R -/- mice. Culturing of the MC-38 mice colon cancer cells. A pilot study with the colon cancer metastatic mice model developed. The MC38 cell line (murine colon adenocarcinoma derived) was injected into the spleen of the same inbred mouse strain. Results: The optimum number of cells to be injected was 3x105 cells for 3 weeks where visible metastasis was observed. Conclusion: A trend was observed in this model where more metastatic tumours we developed in mice with intact Cysltr1 than the mice that lacked the Cysltr1. This model would aid in analysing the role of CysLT1R in metastatic colon cancer.},
  author       = {Devarajan, Anuja},
  language     = {eng},
  note         = {Student Paper},
  title        = {Metastatic model of colon cancer in wild type and cysteinyl leukotriene receptor 1-/- B6 mice},
  year         = {2017},
}