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Investigating self-compatibility of diploid potato populations

van Sloten, Megiel (2018) BIOM02 20172
Degree Projects in Biology
Abstract (Swedish)
Diploid potato cultivars that are propagated by True Potato Seeds (TPS) are a new potentially revolutionary way of potato breeding. Conventional breeding is based on highly heterozygous tetraploid potato varieties. Diploid potato breeding by TPS is useful to create inbred lines and from there hybrid offspring. TPS is cheaper, has almost no diseases and is easy to distribute. Diploid potatoes have a functioning self-incompatibility system that prevents inbreeding. To overcome SI, the Sli gene locus (S locus inhibitor) is introduced in the germplasm to create self-compatible offspring. In this research we will categorize diploid potato populations on their self-compatibility. With the results we will reveal the allelic state of two (F4)... (More)
Diploid potato cultivars that are propagated by True Potato Seeds (TPS) are a new potentially revolutionary way of potato breeding. Conventional breeding is based on highly heterozygous tetraploid potato varieties. Diploid potato breeding by TPS is useful to create inbred lines and from there hybrid offspring. TPS is cheaper, has almost no diseases and is easy to distribute. Diploid potatoes have a functioning self-incompatibility system that prevents inbreeding. To overcome SI, the Sli gene locus (S locus inhibitor) is introduced in the germplasm to create self-compatible offspring. In this research we will categorize diploid potato populations on their self-compatibility. With the results we will reveal the allelic state of two (F4) inbred pop-ulations for the self-compatibility factor (Sli gene) and if we can use the parents of the used diploid populations for further mapping studies. (Less)
Popular Abstract
Are potatoes, grown from seeds, the future?

Did you know that you can grow potato plants from seeds? A potato plant has two ways to propagate itself; by the all known potato that we love to eat and by seeds that are grown in tomato-like berries above the ground. These seed producing potato plants that are as well diploid (two copies of every chromosome) instead of conventional tetraploid (4 copies), are a new potential revolutionary way of potato breeding.

This new way of potato breeding lowers production costs of potatoes, have almost no diseases and you only need 100 grams of seeds for one hectare instead of two tons of normal potatoes. Enough reasons to look for ways to implement this revolutionary method in potato breeding.

... (More)
Are potatoes, grown from seeds, the future?

Did you know that you can grow potato plants from seeds? A potato plant has two ways to propagate itself; by the all known potato that we love to eat and by seeds that are grown in tomato-like berries above the ground. These seed producing potato plants that are as well diploid (two copies of every chromosome) instead of conventional tetraploid (4 copies), are a new potential revolutionary way of potato breeding.

This new way of potato breeding lowers production costs of potatoes, have almost no diseases and you only need 100 grams of seeds for one hectare instead of two tons of normal potatoes. Enough reasons to look for ways to implement this revolutionary method in potato breeding.

The conventional technique that is used till now is based on vegetative propagation and uses tetraploid potato varieties. With this technique breeding is based on crossings between cultivars and different rounds of selection. This takes a lot of time with small success. Therefore, the varieties that are the most popular nowadays are more than 100 years old!

The revolutionary new way of potato breeding that Solynta is working on is much more promising. They are working with a new breeding technique that is using diploid seed-setting potatoes. Here, bypassing a preventive system against self-pollination gives opportunities to create homozygous (having identical alleles for a single trait) parent lines. These parent lines can be selected on desirable phenotypic features based on genetics like resistance, colour of potatoes, starch content etcetera. Crossing of parent lines will give hybrid offspring with desired traits. This means, that when you have the parent lines with specific traits, adaptation to fast changes can be accomplished (e.g. introducing disease resistance).

In this study, several small populations were evaluated for their further usefulness as mapping populations. We looked at self-fertility (ability to fertilise own flowers). That is important to create homozygous parent lines. In other words, can we use the parents of the used diploid populations for further mapping studies?

For the results of this study we looked at the phenotypic characteristics of the individual plants in a population. Based on the self-fertility (and self-infertility) of the populations used in this study, we found that the used two parents, have a different pattern in self-fertility. One is heterozygous, and one is homozygous for the self-fertility allele. These results give a better insight of the parent plants and might be used in future studies.

Using seeds to grow potatoes is a relatively new way of potato breeding but is very promising. This way of breeding gives breeders a method to make fast progress in potato breeding and develop potato varieties with desired traits. This was never possible with the conventional breeding techniques. Of course, this research is only a small part of a bigger study toward the goal that Solynta has in mind. Hybrid seed potatoes that will compete with the conventional way of potato breeding. Potatoes grown from seeds can be the future!

Advisor: Torbjörn Säll
The Section of Plant Science
Department of Biology
Solynta, hybrid potato breeding
Wageningen, the Netherlands (Less)
Please use this url to cite or link to this publication:
author
van Sloten, Megiel
supervisor
organization
course
BIOM02 20172
year
type
H2 - Master's Degree (Two Years)
subject
language
English
id
8937980
date added to LUP
2018-03-23 15:00:10
date last changed
2018-03-23 15:00:10
@misc{8937980,
  abstract     = {Diploid potato cultivars that are propagated by True Potato Seeds (TPS) are a new potentially revolutionary way of potato breeding. Conventional breeding is based on highly heterozygous tetraploid potato varieties. Diploid potato breeding by TPS is useful to create inbred lines and from there hybrid offspring. TPS is cheaper, has almost no diseases and is easy to distribute. Diploid potatoes have a functioning self-incompatibility system that prevents inbreeding. To overcome SI, the Sli gene locus (S locus inhibitor) is introduced in the germplasm to create self-compatible offspring. In this research we will categorize diploid potato populations on their self-compatibility. With the results we will reveal the allelic state of two (F4) inbred pop-ulations for the self-compatibility factor (Sli gene) and if we can use the parents of the used diploid populations for further mapping studies.},
  author       = {van Sloten, Megiel},
  language     = {eng},
  note         = {Student Paper},
  title        = {Investigating self-compatibility of diploid potato populations},
  year         = {2018},
}