Characterization of Mycobacterium sp. MS1601, a bacterium performing selective oxidation of polyols
(2018) MOBN02 20172Degree Projects in Molecular Biology
- Popular Abstract
- A novel promising Mycobacterium Species
Bio-based platform chemicals and polymers have drawn great attention nowadays because of their environmental and economic impacts, compared to those obtained from the fossil. These can be produced from renewable resources, and further transformed to value-added products by enzymes and microorganisms. For example, Mycobacterium sp. MS1601 has the capability to selectively oxidize polyols to corresponding hydroxyl-carboxylic acids, building blocks for manufacturing plastics and resins.
Meanwhile, chemical oxidations of polyols are difficult to achieve the high selective transformation and product yield, and environmentally hazardous in comparison with biological processes. Therefore, the... (More) - A novel promising Mycobacterium Species
Bio-based platform chemicals and polymers have drawn great attention nowadays because of their environmental and economic impacts, compared to those obtained from the fossil. These can be produced from renewable resources, and further transformed to value-added products by enzymes and microorganisms. For example, Mycobacterium sp. MS1601 has the capability to selectively oxidize polyols to corresponding hydroxyl-carboxylic acids, building blocks for manufacturing plastics and resins.
Meanwhile, chemical oxidations of polyols are difficult to achieve the high selective transformation and product yield, and environmentally hazardous in comparison with biological processes. Therefore, the biological approaches specifically attract interests much; however, it is very important to identify the detail characteristics of the microorganisms before using for industrial purpose.
In this study, Mycobacterium sp. MS1601 was classified at the species level, and characterized by different specific tests.Based on the results, the bacterium was classified as a fast growing Mycobacterium sp., a relatively safe and nonpathogenic organism requiring biosafety level-I for laboratory use. And DNA based analysis ensured as a new mycobacterial species. Different growth conditions such as optimum temperature, pH and carbon sources, and it´s characteristics were also studied. Finally, one responsible enzyme for the oxidation of polyol was identified and overproduced.
The study can specifically open the door to biotechnologically use this microorganism for large scale production and biotransformation of bio-based products.
Master’s Degree Project in Molecular Biology 45 credits 2018
Department of Biology, Lund University
Advisor: Dr. Sang-Hyun Pyo
Associate Professor, Division of Biotechnology Department of Chemistry, Faculty of Engineering, Lund University (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/8958898
- author
- Haque, Akm Fahmidul
- supervisor
- organization
- course
- MOBN02 20172
- year
- 2018
- type
- H2 - Master's Degree (Two Years)
- subject
- language
- English
- id
- 8958898
- date added to LUP
- 2018-09-17 13:41:05
- date last changed
- 2018-09-17 13:41:05
@misc{8958898, author = {{Haque, Akm Fahmidul}}, language = {{eng}}, note = {{Student Paper}}, title = {{Characterization of Mycobacterium sp. MS1601, a bacterium performing selective oxidation of polyols}}, year = {{2018}}, }