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The Effect of NK-cells on Viability of Pretreated Pancreatic Cancer Cells Grown in a 3D culture.

Sjösten, Alexandra LU (2018) KIMM05 20181
Department of Immunotechnology
Abstract
Natural killer cells are part of the immune system and one of their main functions is to dispose of cancer cells and virus-infected cells. In order to avoid detection by T cells, cancer cells can decrease the expression of MHC I proteins which instead can make them a target for NK cells, making NK cells a suitable for use in therapy. This study sought to investigate the cytotoxic ability of NK cells in a novel system of co-culturing cells in 3D. The pancreatic ductal adenocarcinoma cell line MiaPaCa-2 and human dermal fibroblasts were grown in a nanometer-sized poly(ε-caprolactone) (PCL) fiber mesh alone and in co-culture and NK cells with effector to target cell ratio of 10-20:1 were added to the cultures. NK cells seemed to affect the... (More)
Natural killer cells are part of the immune system and one of their main functions is to dispose of cancer cells and virus-infected cells. In order to avoid detection by T cells, cancer cells can decrease the expression of MHC I proteins which instead can make them a target for NK cells, making NK cells a suitable for use in therapy. This study sought to investigate the cytotoxic ability of NK cells in a novel system of co-culturing cells in 3D. The pancreatic ductal adenocarcinoma cell line MiaPaCa-2 and human dermal fibroblasts were grown in a nanometer-sized poly(ε-caprolactone) (PCL) fiber mesh alone and in co-culture and NK cells with effector to target cell ratio of 10-20:1 were added to the cultures. NK cells seemed to affect the number of cancer cells in the cultures both in the presence and absence of the activating cytokine IL-2. To investigate the possibility of using NK cells as a combination treatment with other drugs, two different compounds were used, a MTH1 inhibitor and an OGG1 inhibitor. The MTH1 inhibitor was found to affect number of cancer cells in the cultures with no additional decrease in number of cells in combination treatment with NK cells. The OGG1 inhibitor was found to have a low effect on number of cells alone but the effect was substantially increased in combination with NK cells. The OGG1 inhibitor was concluded to show a higher potential as combination treatment with NK cells. (Less)
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author
Sjösten, Alexandra LU
supervisor
organization
course
KIMM05 20181
year
type
H2 - Master's Degree (Two Years)
subject
language
English
id
8962918
date added to LUP
2018-11-15 11:45:33
date last changed
2018-11-15 11:45:33
@misc{8962918,
  abstract     = {{Natural killer cells are part of the immune system and one of their main functions is to dispose of cancer cells and virus-infected cells. In order to avoid detection by T cells, cancer cells can decrease the expression of MHC I proteins which instead can make them a target for NK cells, making NK cells a suitable for use in therapy. This study sought to investigate the cytotoxic ability of NK cells in a novel system of co-culturing cells in 3D. The pancreatic ductal adenocarcinoma cell line MiaPaCa-2 and human dermal fibroblasts were grown in a nanometer-sized poly(ε-caprolactone) (PCL) fiber mesh alone and in co-culture and NK cells with effector to target cell ratio of 10-20:1 were added to the cultures. NK cells seemed to affect the number of cancer cells in the cultures both in the presence and absence of the activating cytokine IL-2. To investigate the possibility of using NK cells as a combination treatment with other drugs, two different compounds were used, a MTH1 inhibitor and an OGG1 inhibitor. The MTH1 inhibitor was found to affect number of cancer cells in the cultures with no additional decrease in number of cells in combination treatment with NK cells. The OGG1 inhibitor was found to have a low effect on number of cells alone but the effect was substantially increased in combination with NK cells. The OGG1 inhibitor was concluded to show a higher potential as combination treatment with NK cells.}},
  author       = {{Sjösten, Alexandra}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{The Effect of NK-cells on Viability of Pretreated Pancreatic Cancer Cells Grown in a 3D culture.}},
  year         = {{2018}},
}