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Electrospun Nanofibrous Polycaprolactone Scaffold for High Throughput Screening of Anti-cancer Drugs in 96-well Plates

Andersson, Nastasia LU (2019) KIMM05 20191
Department of Immunotechnology
Abstract
Cell culturing is most commonly performed in conventional 96-well plates where cells are grown in a monolayer, on two-dimensional (2D) surfaces. However, a tumor grows in three dimensions (3D) in the human body and exhibits complex features that cannot be translated onto a 2D system. Likewise, an anti-cancer drug that has been pre-clinically tested in a 2D system is likely to fail clinical trials. Providing a more physiologically relevant model for cell growth in 3D would thus potentially lead to faster identification of effective anti-cancer drugs, thereby reducing the unsustainable costs associated with drug development. In this study, a novel synthetic polymer scaffold made from electrospun polycaprolactone (PCL) fibers has been... (More)
Cell culturing is most commonly performed in conventional 96-well plates where cells are grown in a monolayer, on two-dimensional (2D) surfaces. However, a tumor grows in three dimensions (3D) in the human body and exhibits complex features that cannot be translated onto a 2D system. Likewise, an anti-cancer drug that has been pre-clinically tested in a 2D system is likely to fail clinical trials. Providing a more physiologically relevant model for cell growth in 3D would thus potentially lead to faster identification of effective anti-cancer drugs, thereby reducing the unsustainable costs associated with drug development. In this study, a novel synthetic polymer scaffold made from electrospun polycaprolactone (PCL) fibers has been developed. Human MiaPaCa-2 pancreatic cancer cells were seeded both in a conventional 96-well plate as well as in 96-well plates containing scaffolds. The cells were treated with the cancer stem cell targeting compound salinomycin. Confocal microscopy images confirmed that the cells were able to migrate and proliferate in the scaffolds. Interestingly, cytotoxicity assays revealed higher IC50 values for cells growing in scaffolds compared to conventional 96-well plates. These results suggest a difference in drug sensitivity related to the positioning of cells in the 3D system. Altogether, these scaffolds may provide researchers with a promising cell culturing alternative for future cancer research and drug development. (Less)
Please use this url to cite or link to this publication:
author
Andersson, Nastasia LU
supervisor
organization
course
KIMM05 20191
year
type
H2 - Master's Degree (Two Years)
subject
language
English
id
8986744
date added to LUP
2019-07-04 14:07:38
date last changed
2019-07-04 14:07:38
@misc{8986744,
  abstract     = {{Cell culturing is most commonly performed in conventional 96-well plates where cells are grown in a monolayer, on two-dimensional (2D) surfaces. However, a tumor grows in three dimensions (3D) in the human body and exhibits complex features that cannot be translated onto a 2D system. Likewise, an anti-cancer drug that has been pre-clinically tested in a 2D system is likely to fail clinical trials. Providing a more physiologically relevant model for cell growth in 3D would thus potentially lead to faster identification of effective anti-cancer drugs, thereby reducing the unsustainable costs associated with drug development. In this study, a novel synthetic polymer scaffold made from electrospun polycaprolactone (PCL) fibers has been developed. Human MiaPaCa-2 pancreatic cancer cells were seeded both in a conventional 96-well plate as well as in 96-well plates containing scaffolds. The cells were treated with the cancer stem cell targeting compound salinomycin. Confocal microscopy images confirmed that the cells were able to migrate and proliferate in the scaffolds. Interestingly, cytotoxicity assays revealed higher IC50 values for cells growing in scaffolds compared to conventional 96-well plates. These results suggest a difference in drug sensitivity related to the positioning of cells in the 3D system. Altogether, these scaffolds may provide researchers with a promising cell culturing alternative for future cancer research and drug development.}},
  author       = {{Andersson, Nastasia}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Electrospun Nanofibrous Polycaprolactone Scaffold for High Throughput Screening of Anti-cancer Drugs in 96-well Plates}},
  year         = {{2019}},
}