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Investigation of the fate of nanodiamonds in cells using STED microscopy

Gösenbauer, Claudia LU (2020) FYSM30 20201
Solid State Physics
Department of Physics
Abstract
Nanodiamonds are novel carbon nanoparticles with favorable properties for biological applications, such as low cytotoxicity and high biocompatibility. They can be used for e.g. cell tracking or drug delivery. The aim of this thesis was to examine the long-term behaviour of nanodiamonds in cells and to compare two different methods of nanodiamond administration, incubation of cells in nanodiamonds and injection of nanodiamonds into cells using a nanoelectroporation setup. The administered nanodiamonds were investigated inside cells using STED microscopy. It was found that nanodiamonds can be delivered to cells with both methods studied. After several days, 40nm nanodiamonds were still detectable in cells after administration via incubation... (More)
Nanodiamonds are novel carbon nanoparticles with favorable properties for biological applications, such as low cytotoxicity and high biocompatibility. They can be used for e.g. cell tracking or drug delivery. The aim of this thesis was to examine the long-term behaviour of nanodiamonds in cells and to compare two different methods of nanodiamond administration, incubation of cells in nanodiamonds and injection of nanodiamonds into cells using a nanoelectroporation setup. The administered nanodiamonds were investigated inside cells using STED microscopy. It was found that nanodiamonds can be delivered to cells with both methods studied. After several days, 40nm nanodiamonds were still detectable in cells after administration via incubation due to substantial clustering. This clustering was not observed after the injection of nanodiamonds into cells using nanoelectroporation or after incubation of cells in 30nm nanodiamonds. Moreover, the colocalisation of nanodiamonds with mitochondria was studied and revealed that the used nanodiamonds do not enter mitochondria. (Less)
Please use this url to cite or link to this publication:
author
Gösenbauer, Claudia LU
supervisor
organization
course
FYSM30 20201
year
type
H2 - Master's Degree (Two Years)
subject
language
English
id
9021085
date added to LUP
2020-07-03 10:12:46
date last changed
2020-07-03 10:12:46
@misc{9021085,
  abstract     = {{Nanodiamonds are novel carbon nanoparticles with favorable properties for biological applications, such as low cytotoxicity and high biocompatibility. They can be used for e.g. cell tracking or drug delivery. The aim of this thesis was to examine the long-term behaviour of nanodiamonds in cells and to compare two different methods of nanodiamond administration, incubation of cells in nanodiamonds and injection of nanodiamonds into cells using a nanoelectroporation setup. The administered nanodiamonds were investigated inside cells using STED microscopy. It was found that nanodiamonds can be delivered to cells with both methods studied. After several days, 40nm nanodiamonds were still detectable in cells after administration via incubation due to substantial clustering. This clustering was not observed after the injection of nanodiamonds into cells using nanoelectroporation or after incubation of cells in 30nm nanodiamonds. Moreover, the colocalisation of nanodiamonds with mitochondria was studied and revealed that the used nanodiamonds do not enter mitochondria.}},
  author       = {{Gösenbauer, Claudia}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Investigation of the fate of nanodiamonds in cells using STED microscopy}},
  year         = {{2020}},
}