Enhanced antibiotic tolerance occurred in multi-species biofilms
(2020) MOBN02 20192Degree Projects in Molecular Biology
- Popular Abstract
- Enhanced antibiotic tolerance occurred in multi-species biofilms
Multi-species biofilms existing in Wastewater Treatment Plant (WWTP) are likely to influence the property of each species, owing to high-density biomass and constant sub-lethal concentration of antibiotics. Research on biofilms had gathered an understanding that biofilms contribute to antibiotic tolerance among bacteria that hinder effective treatment against infections in clinical settings. Therefore, WWTP is considered a hotspot for antibiotic dissemination and an excellent environment to study the interaction of multi-species biofilms and antibiotic tolerance.
Four species Pseudochrobactrum saccharolyticum, Pandoraea sp., Brevundimonas genera, Pseudomonas... (More) - Enhanced antibiotic tolerance occurred in multi-species biofilms
Multi-species biofilms existing in Wastewater Treatment Plant (WWTP) are likely to influence the property of each species, owing to high-density biomass and constant sub-lethal concentration of antibiotics. Research on biofilms had gathered an understanding that biofilms contribute to antibiotic tolerance among bacteria that hinder effective treatment against infections in clinical settings. Therefore, WWTP is considered a hotspot for antibiotic dissemination and an excellent environment to study the interaction of multi-species biofilms and antibiotic tolerance.
Four species Pseudochrobactrum saccharolyticum, Pandoraea sp., Brevundimonas genera, Pseudomonas cuatrocienegasensis, isolated from two Danish WWTPs, were screened for synergistic effect among the co-cultured biofilms through crystal violet. One of the species Pseudomonas cuatrocienegasensis was engineered by inserting antibiotic-resistance gene chromosomally via Tn7 transposition. Biofilm assays were used to evaluate the difference of antibiotic tolerance within mono- and multi-species biofilms.
The mutant species Pseudomonas cuatrocienegasensis::cat was established to display positive growth fitness. The results from this study have shown that the diversity of species in multi-species biofilms contributes to the increased yield of biofilm biomass and facilitates the tolerance of biofilm towards antibiotics. Additionally, the impact of antibiotics on the environmental bacteria reveals the environmental risk of discharged effluent. Hence, this study suggests that the existence of antibiotics in sewage promotes antibiotic tolerance amongst environmental bacteria. Therefore, with these findings, a better understanding of biofilms can be established regarding antibiotic tolerance, bringing the prospect to a more effective strategy to control it.
Master’s Degree Project in Molecular Biology 45 credits 2020
Department of Biology, Lund University
Advisor: Jonas Madsen; Ana Silva
Advisors Department: Section of Microbiology, University of Copenhagen (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9028275
@misc{9028275,
author = {{Li, Xinyu}},
language = {{eng}},
note = {{Student Paper}},
title = {{Enhanced antibiotic tolerance occurred in multi-species biofilms}},
year = {{2020}},
}