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Metabolite profiling to study the impact of caffeine on liver lipid metabolism

Frilund, Jasper-Peter (2020) BION03 20181
Degree Projects in Biology
Abstract
Caffeine is a compound many consume, mostly in the form of coffee, which is not just revitalizing, but has also been shown to have a potential protective effect against diseases such as Alzheimer and Type 2 Diabetes. This project looked at caffeine and its breakdown product paraxanthine, using metabolomics, a technique that determines small compounds called metabolites. This project aimed to investigate the effects of caffeine, with focus on lipid metabolism in liver and blood plasma samples from rabbit pups.
First, we performed a method optimization by comparing established protocols for the homogenization of liver tissue, as well as by evaluating five different methods to determine an appropriate method to extract lipids and metabolites... (More)
Caffeine is a compound many consume, mostly in the form of coffee, which is not just revitalizing, but has also been shown to have a potential protective effect against diseases such as Alzheimer and Type 2 Diabetes. This project looked at caffeine and its breakdown product paraxanthine, using metabolomics, a technique that determines small compounds called metabolites. This project aimed to investigate the effects of caffeine, with focus on lipid metabolism in liver and blood plasma samples from rabbit pups.
First, we performed a method optimization by comparing established protocols for the homogenization of liver tissue, as well as by evaluating five different methods to determine an appropriate method to extract lipids and metabolites from liver and plasma samples. Testing was also performed to find the best method suitable for the usage of an automated pipetting platform.
The results revealed that several lipids and metabolite species were changed in response to caffeine treatment. For instance, various fatty acid species were decreased in liver samples from rabbits treated with caffeine. In plasma samples, on the other hand, fatty acids were increased in response to caffeine treatment. Importantly we also found that age, sex, and feeding of the rabbit pups had an effect on metabolism and hence interfered with the obtained results. Overall, the results of this study indicate that caffeine affects metabolism in liver and blood samples from rabbit pups. (Less)
Popular Abstract
The impact of caffeine on metabolite remodeling in the rabbit

Long have people enjoyed caffeine and its effects, which can be more than just to revitalize and refresh. This project aims to investigate the influence of caffeine and its breakdown product paraxanthine on metabolites, which are small compounds that are present in our blood and all tissues.

To investigate the impact of caffeine on the organism, in this project we determined various lipids, such as fatty acids and cholesterol, and metabolites, like sugars and amino acids. The composition of metabolites and lipids can reveal many things, such as the general state of the organism or future susceptibility to various diseases. Previous studies have shown that caffeine can have... (More)
The impact of caffeine on metabolite remodeling in the rabbit

Long have people enjoyed caffeine and its effects, which can be more than just to revitalize and refresh. This project aims to investigate the influence of caffeine and its breakdown product paraxanthine on metabolites, which are small compounds that are present in our blood and all tissues.

To investigate the impact of caffeine on the organism, in this project we determined various lipids, such as fatty acids and cholesterol, and metabolites, like sugars and amino acids. The composition of metabolites and lipids can reveal many things, such as the general state of the organism or future susceptibility to various diseases. Previous studies have shown that caffeine can have an impact on composition of metabolites and lipids, which has been linked to beneficial effects for chronic diseases such as Alzheimer´s disease and diabetes.

Using a highly advanced technique named mass spectrometry, we determined changes in metabolites and lipids in liver and blood samples from a total of 111 rabbit pups. First, we performed a method optimization, where we compared five established protocols to evaluate which method to extract lipids and metabolites from the samples gave the best results. From this optimization, the results revealed that the method using methanol was the best protocol, which was subsequently used in our experiments.

The present results indicate that several metabolites and lipids, such as fatty acids, were slightly decreased in liver samples from caffeine-treated rabbits when compared to untreated animals. These fatty acids were increased in blood samples, on the other hand, which could suggest that caffeine induces a remodeling of lipids in the liver, which are then transported via the blood stream. Importantly, we found that the effect of age, sex, and different feeding methods of the rabbits was greater than expected and interfered with the results that we observed in response to the caffeine treatment.


Master’s Degree Project in Biology 60 credits 2020
Department of Biology, Lund University


Advisor: Katharina Herzog
Chemistry department/ Centre for Analysis and Synthesis (Less)
Please use this url to cite or link to this publication:
author
Frilund, Jasper-Peter
supervisor
organization
course
BION03 20181
year
type
H2 - Master's Degree (Two Years)
subject
language
English
id
9038403
date added to LUP
2021-01-27 15:52:34
date last changed
2021-01-27 15:52:34
@misc{9038403,
  abstract     = {Caffeine is a compound many consume, mostly in the form of coffee, which is not just revitalizing, but has also been shown to have a potential protective effect against diseases such as Alzheimer and Type 2 Diabetes. This project looked at caffeine and its breakdown product paraxanthine, using metabolomics, a technique that determines small compounds called metabolites. This project aimed to investigate the effects of caffeine, with focus on lipid metabolism in liver and blood plasma samples from rabbit pups.
First, we performed a method optimization by comparing established protocols for the homogenization of liver tissue, as well as by evaluating five different methods to determine an appropriate method to extract lipids and metabolites from liver and plasma samples. Testing was also performed to find the best method suitable for the usage of an automated pipetting platform.
The results revealed that several lipids and metabolite species were changed in response to caffeine treatment. For instance, various fatty acid species were decreased in liver samples from rabbits treated with caffeine. In plasma samples, on the other hand, fatty acids were increased in response to caffeine treatment. Importantly we also found that age, sex, and feeding of the rabbit pups had an effect on metabolism and hence interfered with the obtained results. Overall, the results of this study indicate that caffeine affects metabolism in liver and blood samples from rabbit pups.},
  author       = {Frilund, Jasper-Peter},
  language     = {eng},
  note         = {Student Paper},
  title        = {Metabolite profiling to study the impact of caffeine on liver lipid metabolism},
  year         = {2020},
}