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Mikrofluidikchipp för exponering av biologiska partiklar för varierande molekylära stimuli

Fritzell, Ebba LU and Mentzer, Annie LU (2022) EEML05 20221
Department of Biomedical Engineering
Abstract
Easy exposure of biological particles for molecular stimuli is of interest when examining the effects of antibiotics on different bacteria or to perform toxicity analysis for biophysical studies of vesicles. Such experiments require advanced equipment that can only be found in laboratories and are vastly time-consuming. A possible solution to the problem could be a “lab-on-a-chip”, which in short is a microfluidic chip where it is possible to perform laboratory tests on the micro-scale. These tests are often performed with techniques in microfluidics. The tests presented in this report are based on a novel microfluidic chip, to generate molecular gradients, consisting of a main channel with side pockets of varying length and width along... (More)
Easy exposure of biological particles for molecular stimuli is of interest when examining the effects of antibiotics on different bacteria or to perform toxicity analysis for biophysical studies of vesicles. Such experiments require advanced equipment that can only be found in laboratories and are vastly time-consuming. A possible solution to the problem could be a “lab-on-a-chip”, which in short is a microfluidic chip where it is possible to perform laboratory tests on the micro-scale. These tests are often performed with techniques in microfluidics. The tests presented in this report are based on a novel microfluidic chip, to generate molecular gradients, consisting of a main channel with side pockets of varying length and width along the main channel. The purpose is to find which dimensions of the side pocket are best suited for capturing cells and thus exposing them to different molecular solutions of different concentrations. The project was carried out at the Department of Biomedical Engineering at Lund University and has been done via laboratory experiments and fluorescence microscopy imaging for further analysis of these. It was concluded that photobleaching is a problem when working with fluorescent substances and measures were taken to avoid it. Regarding the chip and its side pockets, it was clear that the wider and deeper pockets gave a better result than the narrower and shallower ones and that there is potential for further development of the chip. (Less)
Please use this url to cite or link to this publication:
author
Fritzell, Ebba LU and Mentzer, Annie LU
supervisor
organization
alternative title
Microfluidic chip for exposure of biological particles to variable molecular stimuli
course
EEML05 20221
year
type
M2 - Bachelor Degree
subject
language
Swedish
id
9083891
date added to LUP
2022-06-10 12:08:49
date last changed
2022-06-10 12:08:49
@misc{9083891,
  abstract     = {{Easy exposure of biological particles for molecular stimuli is of interest when examining the effects of antibiotics on different bacteria or to perform toxicity analysis for biophysical studies of vesicles. Such experiments require advanced equipment that can only be found in laboratories and are vastly time-consuming. A possible solution to the problem could be a “lab-on-a-chip”, which in short is a microfluidic chip where it is possible to perform laboratory tests on the micro-scale. These tests are often performed with techniques in microfluidics. The tests presented in this report are based on a novel microfluidic chip, to generate molecular gradients, consisting of a main channel with side pockets of varying length and width along the main channel. The purpose is to find which dimensions of the side pocket are best suited for capturing cells and thus exposing them to different molecular solutions of different concentrations. The project was carried out at the Department of Biomedical Engineering at Lund University and has been done via laboratory experiments and fluorescence microscopy imaging for further analysis of these. It was concluded that photobleaching is a problem when working with fluorescent substances and measures were taken to avoid it. Regarding the chip and its side pockets, it was clear that the wider and deeper pockets gave a better result than the narrower and shallower ones and that there is potential for further development of the chip.}},
  author       = {{Fritzell, Ebba and Mentzer, Annie}},
  language     = {{swe}},
  note         = {{Student Paper}},
  title        = {{Mikrofluidikchipp för exponering av biologiska partiklar för varierande molekylära stimuli}},
  year         = {{2022}},
}