Improving Resolution In NMR In The Presence of Microcarriers Using Holmium-DOTA
(2024) EEML05 20241Department of Biomedical Engineering
- Abstract
- This research is part of a larger project exploring
the use of surface modified microcarriers, specifically polystyrene
and PDMS, as a chromatographic medium to enable high
resolution liquid state NMR-analysis of biological samples such
as blood and plasma. The purpose of the microcarriers is to
bind proteins and lipids, which distorts the NMR-spectrum,
hence enabling identification of metabolites. This would enable
for direct NMR-analysis of biological samples which would
reduce procedure complexity, operation duration from several
hours to a couple of minutes and finally usage and waste of
organic solvents. The primary objective is to determine whether
adding a paramagnetic solvent to a suspension of water and
microcarriers... (More) - This research is part of a larger project exploring
the use of surface modified microcarriers, specifically polystyrene
and PDMS, as a chromatographic medium to enable high
resolution liquid state NMR-analysis of biological samples such
as blood and plasma. The purpose of the microcarriers is to
bind proteins and lipids, which distorts the NMR-spectrum,
hence enabling identification of metabolites. This would enable
for direct NMR-analysis of biological samples which would
reduce procedure complexity, operation duration from several
hours to a couple of minutes and finally usage and waste of
organic solvents. The primary objective is to determine whether
adding a paramagnetic solvent to a suspension of water and
microcarriers can achieve high resolution NMR-peaks, through
so called magnetic homogeneity. Experiments were conducted at
the Biomedical Center, Lund University, where a NMR tube was
filled with a microcarrier-water suspension and small amounts
of fumarate, used to measure NMR peak linewidth, as a measure
of resolution. To achieve homogeneity a solution of holmium
DOTA, Ho-DOTA, was incrementally added to the tube. After
each addition, the sample was mixed thoroughly and NMR
analysis was performed to measure linewidth changes. The results
suggest that adding Ho-DOTA can significantly enhance magnetic
homogeneity and NMR resolution. In the PDMS suspension a
minimum linewidth of 22Hz was obtained in proton-NMR and
8Hz in 13C NMR spectroscopy. This finding holds potential for
streamlining the analysis of complex biological samples, reducing
the time and complexity involved in traditional NMR preparation
methods. However, in the polystyrene solution line broadening
was only achieved. Althought, this results suggest that our method
might also be used to measure the magnetic susceptibility of an
unknown material. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9160699
- author
- Hasselberg, Fredrik LU and Laurell, Niklas LU
- supervisor
- organization
- alternative title
- Förbättring av upplösning i NMR i närvaro av Mikrobärare genom att använda Holmium-DOTA
- course
- EEML05 20241
- year
- 2024
- type
- M2 - Bachelor Degree
- subject
- language
- English
- id
- 9160699
- date added to LUP
- 2024-06-20 12:34:05
- date last changed
- 2024-06-20 12:34:05
@misc{9160699, abstract = {{This research is part of a larger project exploring the use of surface modified microcarriers, specifically polystyrene and PDMS, as a chromatographic medium to enable high resolution liquid state NMR-analysis of biological samples such as blood and plasma. The purpose of the microcarriers is to bind proteins and lipids, which distorts the NMR-spectrum, hence enabling identification of metabolites. This would enable for direct NMR-analysis of biological samples which would reduce procedure complexity, operation duration from several hours to a couple of minutes and finally usage and waste of organic solvents. The primary objective is to determine whether adding a paramagnetic solvent to a suspension of water and microcarriers can achieve high resolution NMR-peaks, through so called magnetic homogeneity. Experiments were conducted at the Biomedical Center, Lund University, where a NMR tube was filled with a microcarrier-water suspension and small amounts of fumarate, used to measure NMR peak linewidth, as a measure of resolution. To achieve homogeneity a solution of holmium DOTA, Ho-DOTA, was incrementally added to the tube. After each addition, the sample was mixed thoroughly and NMR analysis was performed to measure linewidth changes. The results suggest that adding Ho-DOTA can significantly enhance magnetic homogeneity and NMR resolution. In the PDMS suspension a minimum linewidth of 22Hz was obtained in proton-NMR and 8Hz in 13C NMR spectroscopy. This finding holds potential for streamlining the analysis of complex biological samples, reducing the time and complexity involved in traditional NMR preparation methods. However, in the polystyrene solution line broadening was only achieved. Althought, this results suggest that our method might also be used to measure the magnetic susceptibility of an unknown material.}}, author = {{Hasselberg, Fredrik and Laurell, Niklas}}, language = {{eng}}, note = {{Student Paper}}, title = {{Improving Resolution In NMR In The Presence of Microcarriers Using Holmium-DOTA}}, year = {{2024}}, }