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LUND UNIVERSITY LIBRARIES

Improving Resolution In NMR In The Presence of Microcarriers Using Holmium-DOTA

Hasselberg, Fredrik LU and Laurell, Niklas LU (2024) EEML05 20241
Department of Biomedical Engineering
Abstract
This research is part of a larger project exploring
the use of surface modified microcarriers, specifically polystyrene
and PDMS, as a chromatographic medium to enable high
resolution liquid state NMR-analysis of biological samples such
as blood and plasma. The purpose of the microcarriers is to
bind proteins and lipids, which distorts the NMR-spectrum,
hence enabling identification of metabolites. This would enable
for direct NMR-analysis of biological samples which would
reduce procedure complexity, operation duration from several
hours to a couple of minutes and finally usage and waste of
organic solvents. The primary objective is to determine whether
adding a paramagnetic solvent to a suspension of water and
microcarriers... (More)
This research is part of a larger project exploring
the use of surface modified microcarriers, specifically polystyrene
and PDMS, as a chromatographic medium to enable high
resolution liquid state NMR-analysis of biological samples such
as blood and plasma. The purpose of the microcarriers is to
bind proteins and lipids, which distorts the NMR-spectrum,
hence enabling identification of metabolites. This would enable
for direct NMR-analysis of biological samples which would
reduce procedure complexity, operation duration from several
hours to a couple of minutes and finally usage and waste of
organic solvents. The primary objective is to determine whether
adding a paramagnetic solvent to a suspension of water and
microcarriers can achieve high resolution NMR-peaks, through
so called magnetic homogeneity. Experiments were conducted at
the Biomedical Center, Lund University, where a NMR tube was
filled with a microcarrier-water suspension and small amounts
of fumarate, used to measure NMR peak linewidth, as a measure
of resolution. To achieve homogeneity a solution of holmium
DOTA, Ho-DOTA, was incrementally added to the tube. After
each addition, the sample was mixed thoroughly and NMR
analysis was performed to measure linewidth changes. The results
suggest that adding Ho-DOTA can significantly enhance magnetic
homogeneity and NMR resolution. In the PDMS suspension a
minimum linewidth of 22Hz was obtained in proton-NMR and
8Hz in 13C NMR spectroscopy. This finding holds potential for
streamlining the analysis of complex biological samples, reducing
the time and complexity involved in traditional NMR preparation
methods. However, in the polystyrene solution line broadening
was only achieved. Althought, this results suggest that our method
might also be used to measure the magnetic susceptibility of an
unknown material. (Less)
Please use this url to cite or link to this publication:
author
Hasselberg, Fredrik LU and Laurell, Niklas LU
supervisor
organization
alternative title
Förbättring av upplösning i NMR i närvaro av Mikrobärare genom att använda Holmium-DOTA
course
EEML05 20241
year
type
M2 - Bachelor Degree
subject
language
English
id
9160699
date added to LUP
2024-06-20 12:34:05
date last changed
2024-06-20 12:34:05
@misc{9160699,
  abstract     = {{This research is part of a larger project exploring
the use of surface modified microcarriers, specifically polystyrene
and PDMS, as a chromatographic medium to enable high
resolution liquid state NMR-analysis of biological samples such
as blood and plasma. The purpose of the microcarriers is to
bind proteins and lipids, which distorts the NMR-spectrum,
hence enabling identification of metabolites. This would enable
for direct NMR-analysis of biological samples which would
reduce procedure complexity, operation duration from several
hours to a couple of minutes and finally usage and waste of
organic solvents. The primary objective is to determine whether
adding a paramagnetic solvent to a suspension of water and
microcarriers can achieve high resolution NMR-peaks, through
so called magnetic homogeneity. Experiments were conducted at
the Biomedical Center, Lund University, where a NMR tube was
filled with a microcarrier-water suspension and small amounts
of fumarate, used to measure NMR peak linewidth, as a measure
of resolution. To achieve homogeneity a solution of holmium
DOTA, Ho-DOTA, was incrementally added to the tube. After
each addition, the sample was mixed thoroughly and NMR
analysis was performed to measure linewidth changes. The results
suggest that adding Ho-DOTA can significantly enhance magnetic
homogeneity and NMR resolution. In the PDMS suspension a
minimum linewidth of 22Hz was obtained in proton-NMR and
8Hz in 13C NMR spectroscopy. This finding holds potential for
streamlining the analysis of complex biological samples, reducing
the time and complexity involved in traditional NMR preparation
methods. However, in the polystyrene solution line broadening
was only achieved. Althought, this results suggest that our method
might also be used to measure the magnetic susceptibility of an
unknown material.}},
  author       = {{Hasselberg, Fredrik and Laurell, Niklas}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Improving Resolution In NMR In The Presence of Microcarriers Using Holmium-DOTA}},
  year         = {{2024}},
}