Separating Circulating Tumor Cells from Blood Utilizing Inertial Focusing
(2025) EEML05 20251Department of Biomedical Engineering
- Abstract
- Knowing whether circulating tumor cells (CTCs) are present in the body and to what extent, may be crucial to get an understanding of disease progression and the chosen method of treatment. Further it is of importance to be able to separate CTCs as they are significant when finding new biomarkers for different cancer types. However the research into this area is held back by non-efficient cell separation techniques. It is difficult to separate CTCs from other cells as they are few in number. In 1 ml blood the number of CTCs are approximately a few hundred while there are millions of red blood cells. This report aims to investigate a method of cell separation that uses inertial focusing. Mock-tests were performed before four laborations with... (More)
- Knowing whether circulating tumor cells (CTCs) are present in the body and to what extent, may be crucial to get an understanding of disease progression and the chosen method of treatment. Further it is of importance to be able to separate CTCs as they are significant when finding new biomarkers for different cancer types. However the research into this area is held back by non-efficient cell separation techniques. It is difficult to separate CTCs from other cells as they are few in number. In 1 ml blood the number of CTCs are approximately a few hundred while there are millions of red blood cells. This report aims to investigate a method of cell separation that uses inertial focusing. Mock-tests were performed before four laborations with whole blood and cultivated prostate cancer cells could take place. When separating cancer cells from blood the maximum observed efficiency of the ClearCell® FX1 System was calculated to be 72.41%. Limitations including reutilization of CTC-chips and particle count uncertainties are some factors that might have affected the final results. Therefore, it would be of interest to include flow cytometry in the method to better understand what particles the different sizes of the counts actually represent. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9207505
- author
- Ängskog, Ella LU and Ternevi, Vendela LU
- supervisor
- organization
- alternative title
- Separation av cirkulerande tumörceller från blod genom tröghetsfokusering
- course
- EEML05 20251
- year
- 2025
- type
- M2 - Bachelor Degree
- subject
- keywords
- CTCs, Inertial focusing
- language
- English
- id
- 9207505
- date added to LUP
- 2025-07-01 09:41:59
- date last changed
- 2025-07-01 09:41:59
@misc{9207505, abstract = {{Knowing whether circulating tumor cells (CTCs) are present in the body and to what extent, may be crucial to get an understanding of disease progression and the chosen method of treatment. Further it is of importance to be able to separate CTCs as they are significant when finding new biomarkers for different cancer types. However the research into this area is held back by non-efficient cell separation techniques. It is difficult to separate CTCs from other cells as they are few in number. In 1 ml blood the number of CTCs are approximately a few hundred while there are millions of red blood cells. This report aims to investigate a method of cell separation that uses inertial focusing. Mock-tests were performed before four laborations with whole blood and cultivated prostate cancer cells could take place. When separating cancer cells from blood the maximum observed efficiency of the ClearCell® FX1 System was calculated to be 72.41%. Limitations including reutilization of CTC-chips and particle count uncertainties are some factors that might have affected the final results. Therefore, it would be of interest to include flow cytometry in the method to better understand what particles the different sizes of the counts actually represent.}}, author = {{Ängskog, Ella and Ternevi, Vendela}}, language = {{eng}}, note = {{Student Paper}}, title = {{Separating Circulating Tumor Cells from Blood Utilizing Inertial Focusing}}, year = {{2025}}, }