Speciation and hybridization in birds - genetic evaluation of an undescribed Paradise Flycatcer using mitochondrial markers and RAD-tag sequencing
(2013) BIOP34 20122Degree Projects in Biology
- Abstract
- ABSTRACT
Five species of Paradise Flycatchers (Terpsiphone) occur in mainland Africa. Colour polymorphism and hybridization make species identification and defining species boundaries challenging. A strange melanistic individual was found in Gashaka-Gumti National Park in Nigeria. The melanistic individual was identified as a T. bedfordi based on morphology. However, only T. viridis and T. rufiventer occur in this area. Hypotheses were that the individual is either: (i) a hybrid of T. rufiventer & T. viridis, (ii) a colour morph, or (iii) that it is an individual of a remnant T. bedfordi population. Phylogenetic analysis using three mitochondrial and one nuclear marker showed that the individual groups in the T. viridis clade. In... (More) - ABSTRACT
Five species of Paradise Flycatchers (Terpsiphone) occur in mainland Africa. Colour polymorphism and hybridization make species identification and defining species boundaries challenging. A strange melanistic individual was found in Gashaka-Gumti National Park in Nigeria. The melanistic individual was identified as a T. bedfordi based on morphology. However, only T. viridis and T. rufiventer occur in this area. Hypotheses were that the individual is either: (i) a hybrid of T. rufiventer & T. viridis, (ii) a colour morph, or (iii) that it is an individual of a remnant T. bedfordi population. Phylogenetic analysis using three mitochondrial and one nuclear marker showed that the individual groups in the T. viridis clade. In contrast, analysis on 26,609 loci (~2,5 Mb sequence for each of three individuals sequenced) obtained by RAD-tag sequencing showed that overall FST between the melanistic individual and the other two species was high. Heterozygosity of the individual was lower than in T. viridis and T. rufiventer. Further structure-, differentiation-, distance-, and cluster analyses supported this pattern, showing that this individual is not closely related to T. viridis or T. rufiventer. The current hypotheses is that the individual is from a remnant T. bedfordi population that has an ancestral connection to T. viridis. This hypothesis explains the mtDNA results, as well as the melanistic plumage, and the low overall heterozygosity (Less) - Abstract
- Popular science summary
Identifying an African Paradise Flycatcher using molecular techniques
Five species of Paradise Flycatchers (Terpsiphone) occur in mainland Africa. Colour polymorphism and hybridization in these birds make species identification and defining species boundaries challenging. A melanistic individual was captured in Gashaka-Gumti National Park in Nigeria, and could not be identified as one of the species occurring in this area.
The melanistic individual was identified as a T. bedfordi based on morphology. However, only T. viridis and T. rufiventer occur in this area. Hypotheses were that the individual is either: (i) a hybrid of T. rufiventer & T. viridis, (ii) a colour morph, or (iii) that it is an individual... (More) - Popular science summary
Identifying an African Paradise Flycatcher using molecular techniques
Five species of Paradise Flycatchers (Terpsiphone) occur in mainland Africa. Colour polymorphism and hybridization in these birds make species identification and defining species boundaries challenging. A melanistic individual was captured in Gashaka-Gumti National Park in Nigeria, and could not be identified as one of the species occurring in this area.
The melanistic individual was identified as a T. bedfordi based on morphology. However, only T. viridis and T. rufiventer occur in this area. Hypotheses were that the individual is either: (i) a hybrid of T. rufiventer & T. viridis, (ii) a colour morph, or (iii) that it is an individual of a remnant T. bedfordi population. Phylogenetic analysis using three mitochondrial and one nuclear marker showed that the individual groups close to T. viridis. However, analysis on 26,609 loci (~2,5 Mega base of sequence for each of three individuals sequenced) obtained by RAD-tag sequencing showed that the melanistic individual and T. viridis are not closely related to each other at all. Heterozygosity of the individual was lower than in T. viridis and T. rufiventer, which is the opposite of what is expected if it would have been a hybrid. Several analyses, including structure-, differentiation-, distance-, and cluster analyses supported this pattern, showing that this individual is not closely related to T. viridis
or T. rufiventer. The current hypotheses is that the individual is from a remnant T. bedfordi population that has an ancestral connection to T. viridis. This hypothesis explains the mtDNA results, as well as the melanistic plumage, and the low overall heterozygosity.
Advisor: Bengt Hansson & Martin Stervander
Master´s Degree Project 60 credits in Molecular Ecology 2013
Department of Biology, Lund University (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/4058181
- author
- Pruisscher, Peter
- supervisor
- organization
- course
- BIOP34 20122
- year
- 2013
- type
- H2 - Master's Degree (Two Years)
- subject
- language
- English
- id
- 4058181
- date added to LUP
- 2013-09-25 10:25:49
- date last changed
- 2013-09-25 10:25:49
@misc{4058181, abstract = {{Popular science summary Identifying an African Paradise Flycatcher using molecular techniques Five species of Paradise Flycatchers (Terpsiphone) occur in mainland Africa. Colour polymorphism and hybridization in these birds make species identification and defining species boundaries challenging. A melanistic individual was captured in Gashaka-Gumti National Park in Nigeria, and could not be identified as one of the species occurring in this area. The melanistic individual was identified as a T. bedfordi based on morphology. However, only T. viridis and T. rufiventer occur in this area. Hypotheses were that the individual is either: (i) a hybrid of T. rufiventer & T. viridis, (ii) a colour morph, or (iii) that it is an individual of a remnant T. bedfordi population. Phylogenetic analysis using three mitochondrial and one nuclear marker showed that the individual groups close to T. viridis. However, analysis on 26,609 loci (~2,5 Mega base of sequence for each of three individuals sequenced) obtained by RAD-tag sequencing showed that the melanistic individual and T. viridis are not closely related to each other at all. Heterozygosity of the individual was lower than in T. viridis and T. rufiventer, which is the opposite of what is expected if it would have been a hybrid. Several analyses, including structure-, differentiation-, distance-, and cluster analyses supported this pattern, showing that this individual is not closely related to T. viridis or T. rufiventer. The current hypotheses is that the individual is from a remnant T. bedfordi population that has an ancestral connection to T. viridis. This hypothesis explains the mtDNA results, as well as the melanistic plumage, and the low overall heterozygosity. Advisor: Bengt Hansson & Martin Stervander Master´s Degree Project 60 credits in Molecular Ecology 2013 Department of Biology, Lund University}}, author = {{Pruisscher, Peter}}, language = {{eng}}, note = {{Student Paper}}, title = {{Speciation and hybridization in birds - genetic evaluation of an undescribed Paradise Flycatcer using mitochondrial markers and RAD-tag sequencing}}, year = {{2013}}, }