Chemical Gradient over Maze-Microsystem for Fungal Hyphal Growth
(2015) BMEL01 20151Department of Biomedical Engineering
- Abstract
- The natural environment of fungal hyphae is both physically and chemically heterogeneous, nevertheless is the fungal growth often studied on a flat surface of a homogeneous medium, for example agar. With the help of the well-established techniques of microsystems, it is possible to make structures that resemble their natural habitat. With microfabrication methods obstacles can be created at the size range of a hypha (5-10 μm). These obstacles can in turn be used to manipulate individual hypha. By the principles of microfluidics, a controlled chemical gradient can be created in the culture chamber.
In this study, we have microfabricated a device that consists of physical obstacles in a form of a maze with a chemical gradient. The purpose... (More) - The natural environment of fungal hyphae is both physically and chemically heterogeneous, nevertheless is the fungal growth often studied on a flat surface of a homogeneous medium, for example agar. With the help of the well-established techniques of microsystems, it is possible to make structures that resemble their natural habitat. With microfabrication methods obstacles can be created at the size range of a hypha (5-10 μm). These obstacles can in turn be used to manipulate individual hypha. By the principles of microfluidics, a controlled chemical gradient can be created in the culture chamber.
In this study, we have microfabricated a device that consists of physical obstacles in a form of a maze with a chemical gradient. The purpose of this system is to study the growth dynamics of a hyphae and their sensing of the chemical environment. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/5469968
- author
- Lam, Sofia LU
- supervisor
-
- Pelle Ohlsson LU
- Edith Hammer LU
- organization
- course
- BMEL01 20151
- year
- 2015
- type
- M2 - Bachelor Degree
- subject
- language
- English
- id
- 5469968
- date added to LUP
- 2015-06-09 13:30:43
- date last changed
- 2015-10-08 09:53:44
@misc{5469968, abstract = {{The natural environment of fungal hyphae is both physically and chemically heterogeneous, nevertheless is the fungal growth often studied on a flat surface of a homogeneous medium, for example agar. With the help of the well-established techniques of microsystems, it is possible to make structures that resemble their natural habitat. With microfabrication methods obstacles can be created at the size range of a hypha (5-10 μm). These obstacles can in turn be used to manipulate individual hypha. By the principles of microfluidics, a controlled chemical gradient can be created in the culture chamber. In this study, we have microfabricated a device that consists of physical obstacles in a form of a maze with a chemical gradient. The purpose of this system is to study the growth dynamics of a hyphae and their sensing of the chemical environment.}}, author = {{Lam, Sofia}}, language = {{eng}}, note = {{Student Paper}}, title = {{Chemical Gradient over Maze-Microsystem for Fungal Hyphal Growth}}, year = {{2015}}, }