Control of Bacillus Subtilis Culivations—Feeding Strategies and the Role of Anti-windup in Mid-ranging control
(2005) In MSc ThesesDepartment of Automatic Control
- Abstract
- The first part of this thesis investigates if probing control can be applied to glucose feeding of the bacteria Bacillus subtilis producing the enzyme xylanase. The experimental work reveals unexpected results, where growth and cell/glucose yield are significantly lower in the feed phase than in the batch phase of a fed-batch cultivation. The reason for this is still unclear, but some hypotheses are presented, mainly problems with the medium composition or changes in glucose and growth dynamics at low glucose concentrations. The probing feeding algorithm achieves the goal of maximum growth under the constraint of avoiding glucose accumulation, but still suffers from the problem of slow growth and low cell/glucose yield. In the second part... (More)
- The first part of this thesis investigates if probing control can be applied to glucose feeding of the bacteria Bacillus subtilis producing the enzyme xylanase. The experimental work reveals unexpected results, where growth and cell/glucose yield are significantly lower in the feed phase than in the batch phase of a fed-batch cultivation. The reason for this is still unclear, but some hypotheses are presented, mainly problems with the medium composition or changes in glucose and growth dynamics at low glucose concentrations. The probing feeding algorithm achieves the goal of maximum growth under the constraint of avoiding glucose accumulation, but still suffers from the problem of slow growth and low cell/glucose yield. In the second part of the thesis it is examined whether anti-windup mechanisms should be included into a mid-ranging controller aimed to decrease temperature to handle saturation of oxygen transfer in bacteria cultivations. Simulations and analysis show that anti-windup has disadvantages in the mid-ranging control setting but may help to ensure stability for faster temperature control loops (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/8847916
- author
- Karlsson, Maria
- supervisor
- organization
- year
- 2005
- type
- H3 - Professional qualifications (4 Years - )
- subject
- publication/series
- MSc Theses
- report number
- TFRT-5745
- ISSN
- 0280-5316
- language
- English
- id
- 8847916
- date added to LUP
- 2016-03-18 09:59:26
- date last changed
- 2016-03-18 09:59:26
@misc{8847916,
abstract = {{The first part of this thesis investigates if probing control can be applied to glucose feeding of the bacteria Bacillus subtilis producing the enzyme xylanase. The experimental work reveals unexpected results, where growth and cell/glucose yield are significantly lower in the feed phase than in the batch phase of a fed-batch cultivation. The reason for this is still unclear, but some hypotheses are presented, mainly problems with the medium composition or changes in glucose and growth dynamics at low glucose concentrations. The probing feeding algorithm achieves the goal of maximum growth under the constraint of avoiding glucose accumulation, but still suffers from the problem of slow growth and low cell/glucose yield. In the second part of the thesis it is examined whether anti-windup mechanisms should be included into a mid-ranging controller aimed to decrease temperature to handle saturation of oxygen transfer in bacteria cultivations. Simulations and analysis show that anti-windup has disadvantages in the mid-ranging control setting but may help to ensure stability for faster temperature control loops}},
author = {{Karlsson, Maria}},
issn = {{0280-5316}},
language = {{eng}},
note = {{Student Paper}},
series = {{MSc Theses}},
title = {{Control of Bacillus Subtilis Culivations—Feeding Strategies and the Role of Anti-windup in Mid-ranging control}},
year = {{2005}},
}