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Basophil Signaling in the Endocrine Pancreas - Implications of beta cell dysfunction/destruction in Diabetes Mellitus

Persson, Emma LU (2015) KIM820 20151
Educational programmes
Department of Immunotechnology
Abstract
Diabetes Mellitus is a generic term for metabolic diseases were insulin levels are insufficient and in type 2 Diabetes Mellitus insulin also fails to stimulate glucose uptake. Recent findings indicate that cells from the innate immune system could be involved in development of both type 1 and type 2 Diabetes Mellitus. Basophilic granulocytes are part of the innate immune system and contribute to host defense towards parasites. In addition, they play an important role as effector cells in allergic reactions. In this study, basophil activation in response to diabetes associated substances was investigated. Histamine and serotonin release were used as markers of activation. Basophils stimulated with palmitate (1mM), as well as with palmitate... (More)
Diabetes Mellitus is a generic term for metabolic diseases were insulin levels are insufficient and in type 2 Diabetes Mellitus insulin also fails to stimulate glucose uptake. Recent findings indicate that cells from the innate immune system could be involved in development of both type 1 and type 2 Diabetes Mellitus. Basophilic granulocytes are part of the innate immune system and contribute to host defense towards parasites. In addition, they play an important role as effector cells in allergic reactions. In this study, basophil activation in response to diabetes associated substances was investigated. Histamine and serotonin release were used as markers of activation. Basophils stimulated with palmitate (1mM), as well as with palmitate (1mM) in combination with high glucose (30mM), showed significant increase in histamine release. Toll-like receptor 2 is a surface receptor known to interact with free fatty acids, such as palmitate, and probably mediates the activation of basophils in the present study. Histamine has been shown to affect pancreatic beta cells and insulin secretion, and in this study, insulin producing INS-1(832/13) cells were cultivated with supernatant from basophils stimulations and results indicates a decrease in insulin secretion. Results from this study indicates that activated basophils could be involved in the development/progression of Diabetes Mellitus. (Less)
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author
Persson, Emma LU
supervisor
organization
course
KIM820 20151
year
type
H2 - Master's Degree (Two Years)
subject
keywords
Innate immune system, Diabetes Mellitus, Basophil
language
English
id
7890357
date added to LUP
2015-09-22 13:17:33
date last changed
2015-09-22 13:17:33
@misc{7890357,
  abstract     = {Diabetes Mellitus is a generic term for metabolic diseases were insulin levels are insufficient and in type 2 Diabetes Mellitus insulin also fails to stimulate glucose uptake. Recent findings indicate that cells from the innate immune system could be involved in development of both type 1 and type 2 Diabetes Mellitus. Basophilic granulocytes are part of the innate immune system and contribute to host defense towards parasites. In addition, they play an important role as effector cells in allergic reactions. In this study, basophil activation in response to diabetes associated substances was investigated. Histamine and serotonin release were used as markers of activation. Basophils stimulated with palmitate (1mM), as well as with palmitate (1mM) in combination with high glucose (30mM), showed significant increase in histamine release. Toll-like receptor 2 is a surface receptor known to interact with free fatty acids, such as palmitate, and probably mediates the activation of basophils in the present study. Histamine has been shown to affect pancreatic beta cells and insulin secretion, and in this study, insulin producing INS-1(832/13) cells were cultivated with supernatant from basophils stimulations and results indicates a decrease in insulin secretion. Results from this study indicates that activated basophils could be involved in the development/progression of Diabetes Mellitus.},
  author       = {Persson, Emma},
  keyword      = {Innate immune system,Diabetes Mellitus,Basophil},
  language     = {eng},
  note         = {Student Paper},
  title        = {Basophil Signaling in the Endocrine Pancreas - Implications of beta cell dysfunction/destruction in Diabetes Mellitus},
  year         = {2015},
}