Basophil Signaling in the Endocrine Pancreas - Implications of beta cell dysfunction/destruction in Diabetes Mellitus
(2015) KIM820 20151Educational programmes, LTH
Department of Immunotechnology
- Abstract
- Diabetes Mellitus is a generic term for metabolic diseases were insulin levels are insufficient and in type 2 Diabetes Mellitus insulin also fails to stimulate glucose uptake. Recent findings indicate that cells from the innate immune system could be involved in development of both type 1 and type 2 Diabetes Mellitus. Basophilic granulocytes are part of the innate immune system and contribute to host defense towards parasites. In addition, they play an important role as effector cells in allergic reactions. In this study, basophil activation in response to diabetes associated substances was investigated. Histamine and serotonin release were used as markers of activation. Basophils stimulated with palmitate (1mM), as well as with palmitate... (More)
- Diabetes Mellitus is a generic term for metabolic diseases were insulin levels are insufficient and in type 2 Diabetes Mellitus insulin also fails to stimulate glucose uptake. Recent findings indicate that cells from the innate immune system could be involved in development of both type 1 and type 2 Diabetes Mellitus. Basophilic granulocytes are part of the innate immune system and contribute to host defense towards parasites. In addition, they play an important role as effector cells in allergic reactions. In this study, basophil activation in response to diabetes associated substances was investigated. Histamine and serotonin release were used as markers of activation. Basophils stimulated with palmitate (1mM), as well as with palmitate (1mM) in combination with high glucose (30mM), showed significant increase in histamine release. Toll-like receptor 2 is a surface receptor known to interact with free fatty acids, such as palmitate, and probably mediates the activation of basophils in the present study. Histamine has been shown to affect pancreatic beta cells and insulin secretion, and in this study, insulin producing INS-1(832/13) cells were cultivated with supernatant from basophils stimulations and results indicates a decrease in insulin secretion. Results from this study indicates that activated basophils could be involved in the development/progression of Diabetes Mellitus. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/7890357
- author
- Persson, Emma LU
- supervisor
- organization
- course
- KIM820 20151
- year
- 2015
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- Innate immune system, Diabetes Mellitus, Basophil
- language
- English
- id
- 7890357
- date added to LUP
- 2015-09-22 13:17:33
- date last changed
- 2015-09-22 13:17:33
@misc{7890357, abstract = {{Diabetes Mellitus is a generic term for metabolic diseases were insulin levels are insufficient and in type 2 Diabetes Mellitus insulin also fails to stimulate glucose uptake. Recent findings indicate that cells from the innate immune system could be involved in development of both type 1 and type 2 Diabetes Mellitus. Basophilic granulocytes are part of the innate immune system and contribute to host defense towards parasites. In addition, they play an important role as effector cells in allergic reactions. In this study, basophil activation in response to diabetes associated substances was investigated. Histamine and serotonin release were used as markers of activation. Basophils stimulated with palmitate (1mM), as well as with palmitate (1mM) in combination with high glucose (30mM), showed significant increase in histamine release. Toll-like receptor 2 is a surface receptor known to interact with free fatty acids, such as palmitate, and probably mediates the activation of basophils in the present study. Histamine has been shown to affect pancreatic beta cells and insulin secretion, and in this study, insulin producing INS-1(832/13) cells were cultivated with supernatant from basophils stimulations and results indicates a decrease in insulin secretion. Results from this study indicates that activated basophils could be involved in the development/progression of Diabetes Mellitus.}}, author = {{Persson, Emma}}, language = {{eng}}, note = {{Student Paper}}, title = {{Basophil Signaling in the Endocrine Pancreas - Implications of beta cell dysfunction/destruction in Diabetes Mellitus}}, year = {{2015}}, }