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Prevalence of Aryl Hydrocarbon Receptor expression in the myeloid cell-line MUTZ-3

Strömberg, Marcus LU (2016) KIM820 20161
Department of Immunotechnology
Educational programmes, LTH
Abstract
Candidate chemicals used by e.g. the pharmaceutical, food and cosmetics industries need to be tested for hazardous effects on human health prior to being released to the market. To stop the increasing prevalence of allergic contact dermatitis (ACD), there is an urgent need to develop and use fast and reliable in vitro methods to replace the current, not very accurate, animal experiments used for this purpose. At the Department of Immunotechnology, the myeloid cell line MUTZ-3 is used as a test platform to screen and analyze various chemicals’ potential to cause sensitization. In this study, we set out to investigate the in vivo relevance of this model, by investigating the prevalence of the Aryl Hydrocarbon receptor (AhR) on MUTZ-3 cells... (More)
Candidate chemicals used by e.g. the pharmaceutical, food and cosmetics industries need to be tested for hazardous effects on human health prior to being released to the market. To stop the increasing prevalence of allergic contact dermatitis (ACD), there is an urgent need to develop and use fast and reliable in vitro methods to replace the current, not very accurate, animal experiments used for this purpose. At the Department of Immunotechnology, the myeloid cell line MUTZ-3 is used as a test platform to screen and analyze various chemicals’ potential to cause sensitization. In this study, we set out to investigate the in vivo relevance of this model, by investigating the prevalence of the Aryl Hydrocarbon receptor (AhR) on MUTZ-3 cells in comparison to other primary human cells. In the body, AhR is involved in the metabolism of xenobiotics and thus, important for mobilization of an effective response to these compounds. The objective was to create a comprehensive view covering the expression levels on several cellular levels, using flow cytometry, qPCR, Western Blotting and gene expression data.
In conclusion, the expression of AhR by MUTZ-3 cells were confirmed using flow cytometry, qPCR and gene expression data analysis. Albeit the expression levels were not as high as those in primary monocytes and in vitro-derived monocyte-derived dendritic cells.
An investigation of the correlation between expression levels of the AhR and the metabolic capability of xenobiotics by the cells would be interesting to perform in order to draw a conclusion of the functionality of AhR in MUTZ-3 cells. (Less)
Popular Abstract (Swedish)
De senaste åren har allergisk kontaktdermatit ökat i förekomst och orsakas då av att en individ kommer i kontakt med en substans som denna har utvecklat en allergi emot. Detta leder ofta till att individen får utslag vid kontaktpunkten, men kan i värsta fall få fatala konsekvenser. För att minska skadan denna sjukdom ger, både ur ett ekonomiskt och socialt perspektiv, finns det ett behov att hitta en bra in vitro model för att uppskatta substansers sensibiliserande förmåga. MUTZ-3 används just nu som basen för en sådan testplattform, men en nyligen publicerad artikel presenterade resultat som ifrågasätter MUTZ-3s förmåga att fungera som in vitro model.
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author
Strömberg, Marcus LU
supervisor
organization
course
KIM820 20161
year
type
H2 - Master's Degree (Two Years)
subject
keywords
MUTZ-3, AhR, Aryl Hydrocarbon receptor, Dendritic cells, Allergic Contact Dermatitis
language
English
id
8892838
date added to LUP
2016-10-17 12:41:08
date last changed
2016-10-17 12:41:08
@misc{8892838,
  abstract     = {Candidate chemicals used by e.g. the pharmaceutical, food and cosmetics industries need to be tested for hazardous effects on human health prior to being released to the market. To stop the increasing prevalence of allergic contact dermatitis (ACD), there is an urgent need to develop and use fast and reliable in vitro methods to replace the current, not very accurate, animal experiments used for this purpose. At the Department of Immunotechnology, the myeloid cell line MUTZ-3 is used as a test platform to screen and analyze various chemicals’ potential to cause sensitization. In this study, we set out to investigate the in vivo relevance of this model, by investigating the prevalence of the Aryl Hydrocarbon receptor (AhR) on MUTZ-3 cells in comparison to other primary human cells. In the body, AhR is involved in the metabolism of xenobiotics and thus, important for mobilization of an effective response to these compounds. The objective was to create a comprehensive view covering the expression levels on several cellular levels, using flow cytometry, qPCR, Western Blotting and gene expression data.
In conclusion, the expression of AhR by MUTZ-3 cells were confirmed using flow cytometry, qPCR and gene expression data analysis. Albeit the expression levels were not as high as those in primary monocytes and in vitro-derived monocyte-derived dendritic cells.
An investigation of the correlation between expression levels of the AhR and the metabolic capability of xenobiotics by the cells would be interesting to perform in order to draw a conclusion of the functionality of AhR in MUTZ-3 cells.},
  author       = {Strömberg, Marcus},
  keyword      = {MUTZ-3,AhR,Aryl Hydrocarbon receptor,Dendritic cells,Allergic Contact Dermatitis},
  language     = {eng},
  note         = {Student Paper},
  title        = {Prevalence of Aryl Hydrocarbon Receptor expression in the myeloid cell-line MUTZ-3},
  year         = {2016},
}