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Characterization of Human Primary Bone Marrow Mesenchymal Stromal Cells Pre and Post Cryopreservation and Investigation of Age-related Variances in Phenotype

Jernberg, Fanny LU (2017) KBK820 20171
Pure and Applied Biochemistry
Abstract
Bone marrow mesenchymal stromal cells (BM-MSCs) are critical for hematopoiesis and have been largely explored for potential stem cell therapies in a variety of diseases. This will inescapably create the need for long term storage via cryopreservation. Nevertheless, the effect of cryopreservation on primary human BM-MSCs remains largely unknown. We demonstrated that the surface marker expression pattern of the MSCs was maintained post cryopreservation. However, the viability and CFU-F capacity of the cells were reduced. Furthermore, we showed that there was an increase in the frequency of CD271+ cells post cryopreservation suggesting that MSCs survive freezing better than other cell types of the mononuclear cell fraction. In addition, we... (More)
Bone marrow mesenchymal stromal cells (BM-MSCs) are critical for hematopoiesis and have been largely explored for potential stem cell therapies in a variety of diseases. This will inescapably create the need for long term storage via cryopreservation. Nevertheless, the effect of cryopreservation on primary human BM-MSCs remains largely unknown. We demonstrated that the surface marker expression pattern of the MSCs was maintained post cryopreservation. However, the viability and CFU-F capacity of the cells were reduced. Furthermore, we showed that there was an increase in the frequency of CD271+ cells post cryopreservation suggesting that MSCs survive freezing better than other cell types of the mononuclear cell fraction. In addition, we investigated the phenotype of healthy MSC in different age groups and found that the expression of several MSC surface markers, such as CD271 and CD51, increase over time. Although the study has provided additional insight, there is still a needed for further research before we can fully understand the human MSC phenotype and development. (Less)
Popular Abstract
Mesenchymal stromal cells (MSCs) are shown to have different characteristics depending on a person’s age. MSCs are also often frozen for storage but this makes it more difficult for the cells to survive afterwards.
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author
Jernberg, Fanny LU
supervisor
organization
course
KBK820 20171
year
type
H2 - Master's Degree (Two Years)
subject
keywords
applied biochemistry, tillämpad biokemi
language
English
id
8913767
date added to LUP
2017-09-18 13:43:28
date last changed
2017-09-18 13:43:28
@misc{8913767,
  abstract     = {Bone marrow mesenchymal stromal cells (BM-MSCs) are critical for hematopoiesis and have been largely explored for potential stem cell therapies in a variety of diseases. This will inescapably create the need for long term storage via cryopreservation. Nevertheless, the effect of cryopreservation on primary human BM-MSCs remains largely unknown. We demonstrated that the surface marker expression pattern of the MSCs was maintained post cryopreservation. However, the viability and CFU-F capacity of the cells were reduced. Furthermore, we showed that there was an increase in the frequency of CD271+ cells post cryopreservation suggesting that MSCs survive freezing better than other cell types of the mononuclear cell fraction. In addition, we investigated the phenotype of healthy MSC in different age groups and found that the expression of several MSC surface markers, such as CD271 and CD51, increase over time. Although the study has provided additional insight, there is still a needed for further research before we can fully understand the human MSC phenotype and development.},
  author       = {Jernberg, Fanny},
  keyword      = {applied biochemistry,tillämpad biokemi},
  language     = {eng},
  note         = {Student Paper},
  title        = {Characterization of Human Primary Bone Marrow Mesenchymal Stromal Cells Pre and Post Cryopreservation and Investigation of Age-related Variances in Phenotype},
  year         = {2017},
}