Characterization of Human Primary Bone Marrow Mesenchymal Stromal Cells Pre and Post Cryopreservation and Investigation of Age-related Variances in Phenotype
(2017) KBK820 20171Pure and Applied Biochemistry
- Abstract
- Bone marrow mesenchymal stromal cells (BM-MSCs) are critical for hematopoiesis and have been largely explored for potential stem cell therapies in a variety of diseases. This will inescapably create the need for long term storage via cryopreservation. Nevertheless, the effect of cryopreservation on primary human BM-MSCs remains largely unknown. We demonstrated that the surface marker expression pattern of the MSCs was maintained post cryopreservation. However, the viability and CFU-F capacity of the cells were reduced. Furthermore, we showed that there was an increase in the frequency of CD271+ cells post cryopreservation suggesting that MSCs survive freezing better than other cell types of the mononuclear cell fraction. In addition, we... (More)
- Bone marrow mesenchymal stromal cells (BM-MSCs) are critical for hematopoiesis and have been largely explored for potential stem cell therapies in a variety of diseases. This will inescapably create the need for long term storage via cryopreservation. Nevertheless, the effect of cryopreservation on primary human BM-MSCs remains largely unknown. We demonstrated that the surface marker expression pattern of the MSCs was maintained post cryopreservation. However, the viability and CFU-F capacity of the cells were reduced. Furthermore, we showed that there was an increase in the frequency of CD271+ cells post cryopreservation suggesting that MSCs survive freezing better than other cell types of the mononuclear cell fraction. In addition, we investigated the phenotype of healthy MSC in different age groups and found that the expression of several MSC surface markers, such as CD271 and CD51, increase over time. Although the study has provided additional insight, there is still a needed for further research before we can fully understand the human MSC phenotype and development. (Less)
- Popular Abstract
- Mesenchymal stromal cells (MSCs) are shown to have different characteristics depending on a person’s age. MSCs are also often frozen for storage but this makes it more difficult for the cells to survive afterwards.
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/8913767
- author
- Jernberg, Fanny LU
- supervisor
- organization
- course
- KBK820 20171
- year
- 2017
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- applied biochemistry, tillämpad biokemi
- language
- English
- id
- 8913767
- date added to LUP
- 2017-09-18 13:43:28
- date last changed
- 2017-09-18 13:43:28
@misc{8913767, abstract = {{Bone marrow mesenchymal stromal cells (BM-MSCs) are critical for hematopoiesis and have been largely explored for potential stem cell therapies in a variety of diseases. This will inescapably create the need for long term storage via cryopreservation. Nevertheless, the effect of cryopreservation on primary human BM-MSCs remains largely unknown. We demonstrated that the surface marker expression pattern of the MSCs was maintained post cryopreservation. However, the viability and CFU-F capacity of the cells were reduced. Furthermore, we showed that there was an increase in the frequency of CD271+ cells post cryopreservation suggesting that MSCs survive freezing better than other cell types of the mononuclear cell fraction. In addition, we investigated the phenotype of healthy MSC in different age groups and found that the expression of several MSC surface markers, such as CD271 and CD51, increase over time. Although the study has provided additional insight, there is still a needed for further research before we can fully understand the human MSC phenotype and development.}}, author = {{Jernberg, Fanny}}, language = {{eng}}, note = {{Student Paper}}, title = {{Characterization of Human Primary Bone Marrow Mesenchymal Stromal Cells Pre and Post Cryopreservation and Investigation of Age-related Variances in Phenotype}}, year = {{2017}}, }