Molecular characterization of High Resistant Starch oat lines
(2023) KBKM01 20231Pure and Applied Biochemistry
- Abstract
- Oats are renowned for their high dietary fibre content and numerous health benefits. As the demand for nutritious diets increases and oats become a more popular dietary staple, it is crucial to have a thorough understanding of the various types of dietary fibre present in oats. To explore the correlation between resistant starch (RS) levels and amylose content in high amylose oat lines, a molecular characterization study was conducted on a mutagenized oat population generated via Ethyl methyl sulphonate (EMS) chemical mutagenesis. Four lines; CT0275, CT0340, CT1132, and CT1138, were identified as having high amylose content compared to the reference oat Belinda. Biochemical assays conducted on the high amylose oat lines revealed that... (More)
- Oats are renowned for their high dietary fibre content and numerous health benefits. As the demand for nutritious diets increases and oats become a more popular dietary staple, it is crucial to have a thorough understanding of the various types of dietary fibre present in oats. To explore the correlation between resistant starch (RS) levels and amylose content in high amylose oat lines, a molecular characterization study was conducted on a mutagenized oat population generated via Ethyl methyl sulphonate (EMS) chemical mutagenesis. Four lines; CT0275, CT0340, CT1132, and CT1138, were identified as having high amylose content compared to the reference oat Belinda. Biochemical assays conducted on the high amylose oat lines revealed that certain lines exhibited variations in the levels of RS. Further characterizations were conducted to better understand the high amylose oat lines, including their total starch, dietary fibres, protein, and oil contents, as well as their viscous properties. It was concluded that additional characterization studies of high resistant starch lines will be necessary to gain a deeper understanding of the effects of RS on starch digestibility and its potential health benefits as part of human diets. (Less)
- Popular Abstract
- Are you curious about the science behind your daily diet? Picture this: a bowl of hearty oats sitting on your breakfast table, ready to fuel your day. But did you know that these unassuming grains have been the subject of groundbreaking research? Imagine oats that aren't just good for your taste buds, but for your entire digestive system. Dietary fibres within oats offer a range of health benefits and have valuable applications in our day to day lives. This cereal possesses several dietary fibres such as beta-glucan and arabinoxylan along with other important macronutrients. A more unexplored dietary fibre is Resistant Starch. Resistant starch is like a superhero for your gut, promoting better digestion and potentially keeping chronic... (More)
- Are you curious about the science behind your daily diet? Picture this: a bowl of hearty oats sitting on your breakfast table, ready to fuel your day. But did you know that these unassuming grains have been the subject of groundbreaking research? Imagine oats that aren't just good for your taste buds, but for your entire digestive system. Dietary fibres within oats offer a range of health benefits and have valuable applications in our day to day lives. This cereal possesses several dietary fibres such as beta-glucan and arabinoxylan along with other important macronutrients. A more unexplored dietary fibre is Resistant Starch. Resistant starch is like a superhero for your gut, promoting better digestion and potentially keeping chronic diseases at bay.
Resistant starch is unlike regular starch, which gets broken down and absorbed in your small intestine, resistant starch resists digestion there. Instead, it travels to your large intestine, where it becomes a source of nourishment for the beneficial bacteria living in your gut. In this study, we looked at oats that were made using a special chemical process and were found to have more of something called amylose. Studies before showed that starches with a lot of amylose are harder for enzymes to break down, which makes them have more resistant starch.
We aimed to uncover the potential connection between amylose and resistant starch. Our hope was that by establishing this link, we could potentially enhance the nutritional value of oats, making them even more advantageous for our well-being. We also examined the composition of these oats, encompassing elements such as total starch, fiber, protein, oil, and viscosity. Through these analyses, our aim was to determine whether these distinctive oats hold the potential to benefit our health and understand potential important applications.
We started by using a handy handheld device to get a basic understanding, and then we used various tests to double-check our findings. What's exciting is that we discovered certain oats with high amounts of resistant starch that resists digestion. These oats also had more protein and a texture that sets them apart from regular oats. Oats with high protein content and good nutrition are getting attention for their potential health benefits. They're being looked at for making plant-based proteins. The texture and viscosity of oat-based products play a crucial role in how consumers perceive and accept them. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9135821
- author
- Kelkar, Harshal Sachin LU
- supervisor
- organization
- course
- KBKM01 20231
- year
- 2023
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- Molecular characterization, Resistant Starch, High amylose, Biochemical assays, Oats, Applied Biochemistry
- language
- English
- id
- 9135821
- date added to LUP
- 2025-01-27 16:30:26
- date last changed
- 2025-01-27 16:30:26
@misc{9135821,
abstract = {{Oats are renowned for their high dietary fibre content and numerous health benefits. As the demand for nutritious diets increases and oats become a more popular dietary staple, it is crucial to have a thorough understanding of the various types of dietary fibre present in oats. To explore the correlation between resistant starch (RS) levels and amylose content in high amylose oat lines, a molecular characterization study was conducted on a mutagenized oat population generated via Ethyl methyl sulphonate (EMS) chemical mutagenesis. Four lines; CT0275, CT0340, CT1132, and CT1138, were identified as having high amylose content compared to the reference oat Belinda. Biochemical assays conducted on the high amylose oat lines revealed that certain lines exhibited variations in the levels of RS. Further characterizations were conducted to better understand the high amylose oat lines, including their total starch, dietary fibres, protein, and oil contents, as well as their viscous properties. It was concluded that additional characterization studies of high resistant starch lines will be necessary to gain a deeper understanding of the effects of RS on starch digestibility and its potential health benefits as part of human diets.}},
author = {{Kelkar, Harshal Sachin}},
language = {{eng}},
note = {{Student Paper}},
title = {{Molecular characterization of High Resistant Starch oat lines}},
year = {{2023}},
}