Characterisation and Toxicity Assessment of Metal Ion-Enriched Nanoparticles
(2024) KASM10 20241Centre for Analysis and Synthesis
- Abstract
- Cancer remains one of the main challenges in healthcare, with profound global impact both in terms of morbidity and mortality. Spago Nanomedical is a Swedish company that has developed innovative metal-ion enriched nanoparticle-based therapies for cancer treatment and diagnosis. These nanoparticles have shown great promise and are currently undergoing clinical trials, however, there persists a desire to further investigate the bio-distribution of the particles. The limitation of the current nanoparticles is that they are incorporating a low amount of metal with relatively low atomic number, resulting in insufficient contrast in computed tomography scan (CT). This study focuses on enhancing the contrast of these nanoparticles for improved... (More)
- Cancer remains one of the main challenges in healthcare, with profound global impact both in terms of morbidity and mortality. Spago Nanomedical is a Swedish company that has developed innovative metal-ion enriched nanoparticle-based therapies for cancer treatment and diagnosis. These nanoparticles have shown great promise and are currently undergoing clinical trials, however, there persists a desire to further investigate the bio-distribution of the particles. The limitation of the current nanoparticles is that they are incorporating a low amount of metal with relatively low atomic number, resulting in insufficient contrast in computed tomography scan (CT). This study focuses on enhancing the contrast of these nanoparticles for improved distribution studies by incorporating elements with higher atomic numbers such as bismuth, gadolinium, hafnium, and lutetium. The report presents an investigation of the metal-loading, characterization, and cytotoxicity assessment of the nanoparticles, laying the foundation for potential biomedical applications. Through a combination of analytical techniques including inductively coupled plasma optical emission spectrometry (ICP-OES), dynamic light scattering (DLS), size exclusion chromatography (SEC), transmission electron microscopy (TEM), and cytotoxicity assays, the successful loading of high atomic number metals into nanoparticles was demonstrated. The nanoparticles exhibited consistent size distribution and morphology, with the incorporation of metal ions theoretically enhancing their contrast properties for potential use in CT-scans. Cytotoxicity assessments revealed minimal toxicity of the metal-loaded nanoparticles, supporting their safety for potential in vivo applications. These findings lay a solid foundation for further research regarding the clinical applications of metal-loaded nanoparticles, including distribution studies. (Less)
- Popular Abstract
- Pioneering Cancer Diagnosis and Therapy using Metal Loaded Nanoparticles
Imagine tiny particles so small they're invisible to the naked eye, yet powerful enough to potentially
revolutionize cancer diagnosis and treatment. That's precisely what researchers at Spago Nanomedical
have been working on. In collaboration with Spago Nanomedical, we detail our successful loading of
these so-called nanoparticles with metals, theoretically enhancing their contrast for imaging
techniques like Computed Tomography scans.
With approximately 10 million people succumbing to cancer annually worldwide, the need for more
accurate diagnostic tools and effective treatment strategies has never been more urgent. Spago
Nanomedical, an innovative research... (More) - Pioneering Cancer Diagnosis and Therapy using Metal Loaded Nanoparticles
Imagine tiny particles so small they're invisible to the naked eye, yet powerful enough to potentially
revolutionize cancer diagnosis and treatment. That's precisely what researchers at Spago Nanomedical
have been working on. In collaboration with Spago Nanomedical, we detail our successful loading of
these so-called nanoparticles with metals, theoretically enhancing their contrast for imaging
techniques like Computed Tomography scans.
With approximately 10 million people succumbing to cancer annually worldwide, the need for more
accurate diagnostic tools and effective treatment strategies has never been more urgent. Spago
Nanomedical, an innovative research company, has developed a nanoparticle-based product to combat
cancer cells. When injected, the product functions by selectively binding to cancer tissue, leading to
an accumulation of nanoparticles in the tumors. While these nanoparticles are designed to selectively
accumulate in cancer tissue, the precise mechanism of how and where the particles end up in the body
is yet to be completely understood. To address this knowledge gap, this thesis aimed to improve the
contrast in distribution studies by loading the nanoparticles with new metals.
The project involved loading nanoparticles with the metals bismuth, gadolinium, hafnium and
lutetium. These loaded particles were then characterized using several analytical techniques to ensure
that the size, shape and chemical composition was suitable. Y et, safety remains paramount, motivating
the assessment of toxicity. This was done by subjecting cell cultures to increasing concentrations of
our nanoparticles and recording their potential harm.
Through the procedures described above, our research confirmed that these metal-loaded
nanoparticles are successfully loaded with the metals, theoretically leading to greater contrast in
computed tomography. Our results also show that the particles pose minimal risk to cells, making
them promising candidates for further studies and potential use in medical applications. Our findings
open doors for more accurate visualization of where the nanoparticles end up within the body of a
patient, paving the way for improved diagnostics and targeted therapies. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9161825
- author
- Gripberg, Nicolai LU and Lundberg, Erik LU
- supervisor
- organization
- course
- KASM10 20241
- year
- 2024
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- materials chemistry, CT, computed tomography scans, toxicity, nanoparticles
- language
- English
- id
- 9161825
- date added to LUP
- 2024-08-29 09:05:51
- date last changed
- 2024-08-29 09:05:51
@misc{9161825,
abstract = {{Cancer remains one of the main challenges in healthcare, with profound global impact both in terms of morbidity and mortality. Spago Nanomedical is a Swedish company that has developed innovative metal-ion enriched nanoparticle-based therapies for cancer treatment and diagnosis. These nanoparticles have shown great promise and are currently undergoing clinical trials, however, there persists a desire to further investigate the bio-distribution of the particles. The limitation of the current nanoparticles is that they are incorporating a low amount of metal with relatively low atomic number, resulting in insufficient contrast in computed tomography scan (CT). This study focuses on enhancing the contrast of these nanoparticles for improved distribution studies by incorporating elements with higher atomic numbers such as bismuth, gadolinium, hafnium, and lutetium. The report presents an investigation of the metal-loading, characterization, and cytotoxicity assessment of the nanoparticles, laying the foundation for potential biomedical applications. Through a combination of analytical techniques including inductively coupled plasma optical emission spectrometry (ICP-OES), dynamic light scattering (DLS), size exclusion chromatography (SEC), transmission electron microscopy (TEM), and cytotoxicity assays, the successful loading of high atomic number metals into nanoparticles was demonstrated. The nanoparticles exhibited consistent size distribution and morphology, with the incorporation of metal ions theoretically enhancing their contrast properties for potential use in CT-scans. Cytotoxicity assessments revealed minimal toxicity of the metal-loaded nanoparticles, supporting their safety for potential in vivo applications. These findings lay a solid foundation for further research regarding the clinical applications of metal-loaded nanoparticles, including distribution studies.}},
author = {{Gripberg, Nicolai and Lundberg, Erik}},
language = {{eng}},
note = {{Student Paper}},
title = {{Characterisation and Toxicity Assessment of Metal Ion-Enriched Nanoparticles}},
year = {{2024}},
}