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Analysis of the effect of Bile Salt Stimulated Lipase and Estrogen in Breast cancer cells using Zebrafish Model

Thomas, Atul Koshy LU (2024) KIMM01 20241
Department of Immunotechnology
Educational programmes, LTH
Abstract
Cancer is a disease condition where the cells proliferate uncontrollably leading to organ failure, metabolic collapse (cachexia), chronic inflammation combined with immune dysregulation, and many more. Out of the most prevailing cancers, breast cancer ranks second in the world of which 75% are diagnosed with hormone-dependent breast cancers. Bile Salt Stimulated Lipase(BSSL), a lipolytic enzyme secreted by the pancreas and mammary glands has various roles in the human body and recent studies claim that it plays a crucial role in the inflammatory process for example in inflammatory joint disorders. The role of BSSL in cancer has not been thoroughly explored but previous studies suggest that breast cancer cells express elevated levels of... (More)
Cancer is a disease condition where the cells proliferate uncontrollably leading to organ failure, metabolic collapse (cachexia), chronic inflammation combined with immune dysregulation, and many more. Out of the most prevailing cancers, breast cancer ranks second in the world of which 75% are diagnosed with hormone-dependent breast cancers. Bile Salt Stimulated Lipase(BSSL), a lipolytic enzyme secreted by the pancreas and mammary glands has various roles in the human body and recent studies claim that it plays a crucial role in the inflammatory process for example in inflammatory joint disorders. The role of BSSL in cancer has not been thoroughly explored but previous studies suggest that breast cancer cells express elevated levels of BSSL. This project analyzed the effect of BSSL and estrogen in two different breast cancer types, hormone-dependent and triple-negative breast cancers using zebrafish cell line-derived xenograft models. The hormone-dependent breast cancer cell line (MCF-7) showed a significant increase in the tumor area when treated with BSSL, and further studies need to be done to unravel the mechanism of tumor progression by BSSL, in the future. (Less)
Popular Abstract
Cancer is a disease that affects different parts of the body and causing various abnormalities that affect the functioning of the human body. According to the reports, breast cancer is the second most common cancer diagnosed worldwide out of which 75% percent of them are hormone dependent breast cancer. Bile Salt Stimulated Lipase, an enzyme secreted mostly by pancreas and mammary glands in humans, have potential in causing inflammatory joint diseases and recent studies have found that BSSL was expressed more in breast cancer cells when compared with normal cells. In this experiment, the role of BSSL in tumor was studied. Zebrafish embryos were injected with two different cancer cells and treated with BSSL and estrogen. From the results,... (More)
Cancer is a disease that affects different parts of the body and causing various abnormalities that affect the functioning of the human body. According to the reports, breast cancer is the second most common cancer diagnosed worldwide out of which 75% percent of them are hormone dependent breast cancer. Bile Salt Stimulated Lipase, an enzyme secreted mostly by pancreas and mammary glands in humans, have potential in causing inflammatory joint diseases and recent studies have found that BSSL was expressed more in breast cancer cells when compared with normal cells. In this experiment, the role of BSSL in tumor was studied. Zebrafish embryos were injected with two different cancer cells and treated with BSSL and estrogen. From the results, it was clear that the cancer cells that respond to the hormone have developed more tumor upon BSSL exposure when compared with cells that are not treated with BSSL. Other cancer cell type that does not respond to the hormones show no effect. In this experiment, the spreading of cancer to other parts of the zebrafish was also analyzed and BSSL also induced tumor spreading during certain conditions. An attempt to study the effect of B cells to the tumor with BSSL was also done but the experiment was not successful. Overall, the experiments were able to show that the BSSL has the capability to promote cancer and may be interesting for future cancer treatment strategies. (Less)
Please use this url to cite or link to this publication:
author
Thomas, Atul Koshy LU
supervisor
organization
course
KIMM01 20241
year
type
H2 - Master's Degree (Two Years)
subject
keywords
Bile Salt Stimulated Lipase, Cancer, Zebrafish embryos, Breast Cancer, Hormone dependent cancer
language
English
id
9175506
date added to LUP
2024-09-27 13:06:46
date last changed
2024-09-27 13:06:46
@misc{9175506,
  abstract     = {{Cancer is a disease condition where the cells proliferate uncontrollably leading to organ failure, metabolic collapse (cachexia), chronic inflammation combined with immune dysregulation, and many more. Out of the most prevailing cancers, breast cancer ranks second in the world of which 75% are diagnosed with hormone-dependent breast cancers. Bile Salt Stimulated Lipase(BSSL), a lipolytic enzyme secreted by the pancreas and mammary glands has various roles in the human body and recent studies claim that it plays a crucial role in the inflammatory process for example in inflammatory joint disorders. The role of BSSL in cancer has not been thoroughly explored but previous studies suggest that breast cancer cells express elevated levels of BSSL. This project analyzed the effect of BSSL and estrogen in two different breast cancer types, hormone-dependent and triple-negative breast cancers using zebrafish cell line-derived xenograft models. The hormone-dependent breast cancer cell line (MCF-7) showed a significant increase in the tumor area when treated with BSSL, and further studies need to be done to unravel the mechanism of tumor progression by BSSL, in the future.}},
  author       = {{Thomas, Atul Koshy}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Analysis of the effect of Bile Salt Stimulated Lipase and Estrogen in Breast cancer cells using Zebrafish Model}},
  year         = {{2024}},
}