Strategies for enhancing fermentative production of glycerol - A review
(2002) In Enzyme and Microbial Technology 31(1-2). p.53-66- Abstract
- The present paper reviews the metabolic basis of different methods for fermentative glycerol production. The most important microbial production organism is the yeast Saccharomyces cerevisiae but other yeast species, as well as molds, algae, and bacteria are of potential interest for glycerol production. A large variety of methods have been applied to increase the fermentative glycerol yield. The first methods were based on physiological control, e.g. chemically induced overproduction of glycerol through NADH entrapment by the addition of chemical steering agents (such as bisulfite). More recently, genetic engineering of the glycolytic pathway has been used to improve production, involving modulated function of e.g. triose phosphate... (More)
- The present paper reviews the metabolic basis of different methods for fermentative glycerol production. The most important microbial production organism is the yeast Saccharomyces cerevisiae but other yeast species, as well as molds, algae, and bacteria are of potential interest for glycerol production. A large variety of methods have been applied to increase the fermentative glycerol yield. The first methods were based on physiological control, e.g. chemically induced overproduction of glycerol through NADH entrapment by the addition of chemical steering agents (such as bisulfite). More recently, genetic engineering of the glycolytic pathway has been used to improve production, involving modulated function of e.g. triose phosphate isomerase, phosphoglycerate mutase, PDC or alcohol dehydrogenase. Direct intervention in the glycerol pathway, such as overexpression of G3P dehydrogenase, has also been tried. The applied strategies can be divided into three principal groups; (a) deactivation or down-regulation of NADH oxidation sites alternative to G3P dehydrogenase, (b) increase of NADH generation or, (c) direct changes in the carbon flux to glycerol. (C) 2002 Published by Elsevier Science Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/332769
- author
- Taherzadeh, MJ ; Adler, L and Lidén, Gunnar LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- genetic engineering, NADH, redox, osmoregulation, yeast, glycerol
- in
- Enzyme and Microbial Technology
- volume
- 31
- issue
- 1-2
- pages
- 53 - 66
- publisher
- Elsevier
- external identifiers
-
- wos:000177018400007
- scopus:0036644259
- ISSN
- 0141-0229
- DOI
- 10.1016/S0141-0229(02)00069-8
- language
- English
- LU publication?
- yes
- id
- 087f627a-e6d5-4c5a-ad84-ea215475706b (old id 332769)
- date added to LUP
- 2016-04-01 11:49:53
- date last changed
- 2023-12-10 00:24:07
@article{087f627a-e6d5-4c5a-ad84-ea215475706b,
abstract = {{The present paper reviews the metabolic basis of different methods for fermentative glycerol production. The most important microbial production organism is the yeast Saccharomyces cerevisiae but other yeast species, as well as molds, algae, and bacteria are of potential interest for glycerol production. A large variety of methods have been applied to increase the fermentative glycerol yield. The first methods were based on physiological control, e.g. chemically induced overproduction of glycerol through NADH entrapment by the addition of chemical steering agents (such as bisulfite). More recently, genetic engineering of the glycolytic pathway has been used to improve production, involving modulated function of e.g. triose phosphate isomerase, phosphoglycerate mutase, PDC or alcohol dehydrogenase. Direct intervention in the glycerol pathway, such as overexpression of G3P dehydrogenase, has also been tried. The applied strategies can be divided into three principal groups; (a) deactivation or down-regulation of NADH oxidation sites alternative to G3P dehydrogenase, (b) increase of NADH generation or, (c) direct changes in the carbon flux to glycerol. (C) 2002 Published by Elsevier Science Inc.}},
author = {{Taherzadeh, MJ and Adler, L and Lidén, Gunnar}},
issn = {{0141-0229}},
keywords = {{genetic engineering; NADH; redox; osmoregulation; yeast; glycerol}},
language = {{eng}},
number = {{1-2}},
pages = {{53--66}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{Strategies for enhancing fermentative production of glycerol - A review}},
url = {{http://dx.doi.org/10.1016/S0141-0229(02)00069-8}},
doi = {{10.1016/S0141-0229(02)00069-8}},
volume = {{31}},
year = {{2002}},
}