Parameter estimation of protein chromatographic processes based on breakthrough curves
(2002) 8th IFAC International Conference on Computer Applications in Biotechnology (CAB8) p.113-118- Abstract
- A methodology for parameter estimation in chromatographic models is suggested in this paper, which is based on breakthrough curves from a given column set-up. Physical parameters are found in two subsequent experiments, one for the mobile phase behaviour and one for the stationary phase behaviour. The estimations are based on flow rate changes and multiple responses. Adsorption parameters are found also in two subsequent experiments, one for isotherm parameters and one for kinetics parameters. These estimations are based on concentration changes. The conclusion is that it is possible to estimate the parameters in a general rate model using nonlinear least square estimation and numerical simulation of the model. Copyright (C) 2001 IFAC.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1407245
- author
- Persson, Patrik LU and Nilsson, Bernt LU
- organization
- publishing date
- 2002
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- simulation, numerical methods, parameter estimation, chromatography, modelling
- host publication
- Computer Applications in Biotechnology 2001 (CAB8)
- pages
- 113 - 118
- publisher
- Elsevier
- conference name
- 8th IFAC International Conference on Computer Applications in Biotechnology (CAB8)
- conference location
- Quebeq City, Canada
- conference dates
- 2001-06-24 - 2001-06-27
- external identifiers
-
- wos:000177564700020
- ISBN
- 0080436811
- language
- English
- LU publication?
- yes
- id
- 6b127a33-27f4-4f4e-bb57-14c436ab3975 (old id 1407245)
- date added to LUP
- 2016-04-04 11:43:56
- date last changed
- 2019-12-11 09:36:45
@inproceedings{6b127a33-27f4-4f4e-bb57-14c436ab3975, abstract = {{A methodology for parameter estimation in chromatographic models is suggested in this paper, which is based on breakthrough curves from a given column set-up. Physical parameters are found in two subsequent experiments, one for the mobile phase behaviour and one for the stationary phase behaviour. The estimations are based on flow rate changes and multiple responses. Adsorption parameters are found also in two subsequent experiments, one for isotherm parameters and one for kinetics parameters. These estimations are based on concentration changes. The conclusion is that it is possible to estimate the parameters in a general rate model using nonlinear least square estimation and numerical simulation of the model. Copyright (C) 2001 IFAC.}}, author = {{Persson, Patrik and Nilsson, Bernt}}, booktitle = {{Computer Applications in Biotechnology 2001 (CAB8)}}, isbn = {{0080436811}}, keywords = {{simulation; numerical methods; parameter estimation; chromatography; modelling}}, language = {{eng}}, pages = {{113--118}}, publisher = {{Elsevier}}, title = {{Parameter estimation of protein chromatographic processes based on breakthrough curves}}, year = {{2002}}, }