Effect of hindered diffusion on the adsorption of proteins in agarose gel using a pore model
(2004) In Journal of Chromatography A 1048(2). p.161-172- Abstract
- The hindered diffusion and binding of proteins of different sizes (lysozyme, BSA and IgG) in an agarose gel is described using adsorption kinetic and diffusional data together with an experimentally determined pore size distribution in the gel. The validity of the pore model, including variable diffusion coefficients and porosities is tested against experimental confocal microscopy data. No fitting parameters were used in the present model. The importance of knowing the gel structure is demonstrated especially for large proteins such as IgG. Experimental confocal microscopy data can be explained by the present model. (C) 2004 Elsevier B.V. All rights reserved.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138799
- author
- Gutenwik, Jan LU ; Nilsson, Bernt LU and Axelsson, Anders LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Chromatography A
- volume
- 1048
- issue
- 2
- pages
- 161 - 172
- publisher
- Elsevier
- external identifiers
-
- pmid:15481253
- wos:000223813800003
- scopus:4444313472
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2004.07.052
- language
- English
- LU publication?
- yes
- id
- 729cf787-0c01-4d48-bdc1-0e71ce353a07 (old id 138799)
- date added to LUP
- 2016-04-01 15:53:37
- date last changed
- 2023-09-04 08:34:29
@article{729cf787-0c01-4d48-bdc1-0e71ce353a07, abstract = {{The hindered diffusion and binding of proteins of different sizes (lysozyme, BSA and IgG) in an agarose gel is described using adsorption kinetic and diffusional data together with an experimentally determined pore size distribution in the gel. The validity of the pore model, including variable diffusion coefficients and porosities is tested against experimental confocal microscopy data. No fitting parameters were used in the present model. The importance of knowing the gel structure is demonstrated especially for large proteins such as IgG. Experimental confocal microscopy data can be explained by the present model. (C) 2004 Elsevier B.V. All rights reserved.}}, author = {{Gutenwik, Jan and Nilsson, Bernt and Axelsson, Anders}}, issn = {{0021-9673}}, language = {{eng}}, number = {{2}}, pages = {{161--172}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{Effect of hindered diffusion on the adsorption of proteins in agarose gel using a pore model}}, url = {{http://dx.doi.org/10.1016/j.chroma.2004.07.052}}, doi = {{10.1016/j.chroma.2004.07.052}}, volume = {{1048}}, year = {{2004}}, }