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Component of the alternative oxidase localized to the matrix surface of the inner membrane of plant mitochondria

Rasmusson, Allan G. LU ; Møller, Ian M. and Palmer, John M. LU (1990) In FEBS Letters 259(2). p.311-314
Abstract

In mitoplasts from Arum maculatum spadices, succinate dehydrogenase (EC 1.3.99.1) and the alternative, cyanide-resistant oxidase activity (measured as m-chlorobenzhydroxamic acid-sensitive duroquinol oxidation) was unaffected by treatment with trypsin. In contrast, when 85% inside-out submitochondrial particles were treated with trypsin the alternative oxidase activity was inhibited by about 50% and succinate dehydrogenase activity by about 40%. Thus, a trypsin-sensitive component of the alternative pathway is located on the inner surface of the inner mitochondrial membrane. After trypsin treatment of the inside-out submitochondrial particles the inhibited alternative oxidase activity was partly restored by including 0.7 M citrate in... (More)

In mitoplasts from Arum maculatum spadices, succinate dehydrogenase (EC 1.3.99.1) and the alternative, cyanide-resistant oxidase activity (measured as m-chlorobenzhydroxamic acid-sensitive duroquinol oxidation) was unaffected by treatment with trypsin. In contrast, when 85% inside-out submitochondrial particles were treated with trypsin the alternative oxidase activity was inhibited by about 50% and succinate dehydrogenase activity by about 40%. Thus, a trypsin-sensitive component of the alternative pathway is located on the inner surface of the inner mitochondrial membrane. After trypsin treatment of the inside-out submitochondrial particles the inhibited alternative oxidase activity was partly restored by including 0.7 M citrate in the assay medium. This indicates that trypsin does not destroy the active site but merely causes a conformational change in the enzyme, thereby lowering its activity.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
(Arum maculatum, Plant, Mitochondria), Alternative pathway, Inside-out submitochondrial particle, Mitoplast, Trypsin
in
FEBS Letters
volume
259
issue
2
pages
4 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0025034768
ISSN
0014-5793
DOI
10.1016/0014-5793(90)80034-G
language
English
LU publication?
yes
id
48323140-1eaf-4921-ba01-353de3ccf445
date added to LUP
2016-05-31 21:43:01
date last changed
2021-01-06 08:39:13
@article{48323140-1eaf-4921-ba01-353de3ccf445,
  abstract     = {{<p>In mitoplasts from Arum maculatum spadices, succinate dehydrogenase (EC 1.3.99.1) and the alternative, cyanide-resistant oxidase activity (measured as m-chlorobenzhydroxamic acid-sensitive duroquinol oxidation) was unaffected by treatment with trypsin. In contrast, when 85% inside-out submitochondrial particles were treated with trypsin the alternative oxidase activity was inhibited by about 50% and succinate dehydrogenase activity by about 40%. Thus, a trypsin-sensitive component of the alternative pathway is located on the inner surface of the inner mitochondrial membrane. After trypsin treatment of the inside-out submitochondrial particles the inhibited alternative oxidase activity was partly restored by including 0.7 M citrate in the assay medium. This indicates that trypsin does not destroy the active site but merely causes a conformational change in the enzyme, thereby lowering its activity.</p>}},
  author       = {{Rasmusson, Allan G. and Møller, Ian M. and Palmer, John M.}},
  issn         = {{0014-5793}},
  keywords     = {{(Arum maculatum, Plant, Mitochondria); Alternative pathway; Inside-out submitochondrial particle; Mitoplast; Trypsin}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{2}},
  pages        = {{311--314}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{Component of the alternative oxidase localized to the matrix surface of the inner membrane of plant mitochondria}},
  url          = {{http://dx.doi.org/10.1016/0014-5793(90)80034-G}},
  doi          = {{10.1016/0014-5793(90)80034-G}},
  volume       = {{259}},
  year         = {{1990}},
}