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Detection of factor IX inhibitors by immunoradiometric assay

Ljung, R LU and Holmberg, Lars LU (1983) In Acta Haematologica 70(5). p.11-307
Abstract

An immunoradiometric assay (IRMA) for the determination of factor IX inhibitors in haemophilia B was developed. The assay was based on competitive binding between radiolabelled anti-IX and inhibitors in the test material of immobilized IX:C. 5 haemophiliacs with known inhibitors were investigated with the new method and with a conventional neutralization test. An inhibitor titre as low as 5 X 10(-4) U/ml could be measured by IRMA, showing it to be about 500 times as sensitive as the conventional neutralization assay used, and in 2 cases the inhibitors could only be detected by IRMA. The new method is thus useful for the investigation of patients with low inhibitor titres, or when inhibitors with divergent properties are suspected.

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author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Antibodies, Binding Sites, Antibody, Binding, Competitive, Blood Coagulation, Factor IX, Hemophilia B, Neutralization Tests, Radioimmunoassay, Journal Article
in
Acta Haematologica
volume
70
issue
5
pages
11 - 307
publisher
Karger
external identifiers
  • Scopus:0020518802
ISSN
0001-5792
language
English
LU publication?
yes
id
bb2f23f9-7bd5-4647-baba-95999aada291
date added to LUP
2017-01-02 08:59:23
date last changed
2017-01-08 06:04:39
@article{bb2f23f9-7bd5-4647-baba-95999aada291,
  abstract     = {<p>An immunoradiometric assay (IRMA) for the determination of factor IX inhibitors in haemophilia B was developed. The assay was based on competitive binding between radiolabelled anti-IX and inhibitors in the test material of immobilized IX:C. 5 haemophiliacs with known inhibitors were investigated with the new method and with a conventional neutralization test. An inhibitor titre as low as 5 X 10(-4) U/ml could be measured by IRMA, showing it to be about 500 times as sensitive as the conventional neutralization assay used, and in 2 cases the inhibitors could only be detected by IRMA. The new method is thus useful for the investigation of patients with low inhibitor titres, or when inhibitors with divergent properties are suspected.</p>},
  author       = {Ljung, R and Holmberg, Lars},
  issn         = {0001-5792},
  keyword      = {Antibodies,Binding Sites, Antibody,Binding, Competitive,Blood Coagulation,Factor IX,Hemophilia B,Neutralization Tests,Radioimmunoassay,Journal Article},
  language     = {eng},
  number       = {5},
  pages        = {11--307},
  publisher    = {Karger},
  series       = {Acta Haematologica},
  title        = {Detection of factor IX inhibitors by immunoradiometric assay},
  volume       = {70},
  year         = {1983},
}