Detection of factor IX inhibitors by immunoradiometric assay
Ljung, R LU
and Holmberg, Lars
LU
(1983)
In Acta Haematologica
70(5).
p.11-307
- Abstract
An immunoradiometric assay (IRMA) for the determination of factor IX inhibitors in haemophilia B was developed. The assay was based on competitive binding between radiolabelled anti-IX and inhibitors in the test material of immobilized IX:C. 5 haemophiliacs with known inhibitors were investigated with the new method and with a conventional neutralization test. An inhibitor titre as low as 5 X 10(-4) U/ml could be measured by IRMA, showing it to be about 500 times as sensitive as the conventional neutralization assay used, and in 2 cases the inhibitors could only be detected by IRMA. The new method is thus useful for the investigation of patients with low inhibitor titres, or when inhibitors with divergent properties are suspected.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/bb2f23f9-7bd5-4647-baba-95999aada291
- author
- Ljung, R
LU
and Holmberg, Lars
LU
- organization
- publishing date
- 1983
- type
- Contribution to journal
- publication status
- published
- keywords
- Antibodies, Binding Sites, Antibody, Binding, Competitive, Blood Coagulation, Factor IX, Hemophilia B, Neutralization Tests, Radioimmunoassay, Journal Article
- in
- Acta Haematologica
- volume
- 70
- issue
- 5
- pages
- 11 - 307
- publisher
- Karger
- external identifiers
-
- pmid:6414224
- scopus:0020518802
- ISSN
- 0001-5792
- language
- English
- LU publication?
- yes
- id
- bb2f23f9-7bd5-4647-baba-95999aada291
- date added to LUP
- 2017-01-02 08:59:23
- date last changed
- 2024-01-04 20:11:38
@article{bb2f23f9-7bd5-4647-baba-95999aada291,
abstract = {{<p>An immunoradiometric assay (IRMA) for the determination of factor IX inhibitors in haemophilia B was developed. The assay was based on competitive binding between radiolabelled anti-IX and inhibitors in the test material of immobilized IX:C. 5 haemophiliacs with known inhibitors were investigated with the new method and with a conventional neutralization test. An inhibitor titre as low as 5 X 10(-4) U/ml could be measured by IRMA, showing it to be about 500 times as sensitive as the conventional neutralization assay used, and in 2 cases the inhibitors could only be detected by IRMA. The new method is thus useful for the investigation of patients with low inhibitor titres, or when inhibitors with divergent properties are suspected.</p>}},
author = {{Ljung, R and Holmberg, Lars}},
issn = {{0001-5792}},
keywords = {{Antibodies; Binding Sites, Antibody; Binding, Competitive; Blood Coagulation; Factor IX; Hemophilia B; Neutralization Tests; Radioimmunoassay; Journal Article}},
language = {{eng}},
number = {{5}},
pages = {{11--307}},
publisher = {{Karger}},
series = {{Acta Haematologica}},
title = {{Detection of factor IX inhibitors by immunoradiometric assay}},
volume = {{70}},
year = {{1983}},
}