Advanced

Preparation of cell suspensions for co-transplantation : methodological considerations

Othberg, A I LU ; Willing, A E; Saporta, S; Cameron, D F and Sanberg, P R (1998) In Neuroscience Letters 247(2-3). p.4-111
Abstract

The purpose of the current study was to determine the optimal strategy for preparing cell suspensions for co-transplantation. In the first experiment, the number of Sertoli cell (SC) aggregates and the number of tyrosine hydroxylase positive neurons were compared over time when cell suspensions of Sertoli or ventral mesencephalic cells were kept as a co-suspension mixed at 0 h. Cells from each suspension were dispensed onto glass slides in a manner similar to transplantation. When dispensed in this manner, the number of SC aggregates and TH-positive neurons decreased over 4 h. In experiment 2, the cell suspensions were mixed just prior to injection at each of four timepoints, the number of aggregates and TH neurons was consistent over... (More)

The purpose of the current study was to determine the optimal strategy for preparing cell suspensions for co-transplantation. In the first experiment, the number of Sertoli cell (SC) aggregates and the number of tyrosine hydroxylase positive neurons were compared over time when cell suspensions of Sertoli or ventral mesencephalic cells were kept as a co-suspension mixed at 0 h. Cells from each suspension were dispensed onto glass slides in a manner similar to transplantation. When dispensed in this manner, the number of SC aggregates and TH-positive neurons decreased over 4 h. In experiment 2, the cell suspensions were mixed just prior to injection at each of four timepoints, the number of aggregates and TH neurons was consistent over time. Clearly this latter strategy resulted consistent recovery of both cell types for transplants up to 3 h after suspension preparation.

(Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Biomarkers, Brain Tissue Transplantation, Cell Aggregation, Cell Culture Techniques, Cell Survival, Cell Transplantation, Coculture Techniques, Fetal Tissue Transplantation, Male, Mesencephalon, Nerve Tissue Proteins, Neurons, Rats, Rats, Sprague-Dawley, Sertoli Cells, Suspensions, Swine, Tyrosine 3-Monooxygenase, Journal Article, Research Support, Non-U.S. Gov't
in
Neuroscience Letters
volume
247
issue
2-3
pages
4 pages
publisher
Elsevier
external identifiers
  • Scopus:0032524283
ISSN
0304-3940
DOI
10.1016/S0304-3940(98)00283-3
language
English
LU publication?
no
id
f636d5d6-1e01-4e63-b1e7-095c08e2545f
date added to LUP
2016-12-11 10:00:36
date last changed
2017-01-01 08:42:55
@article{f636d5d6-1e01-4e63-b1e7-095c08e2545f,
  abstract     = {<p>The purpose of the current study was to determine the optimal strategy for preparing cell suspensions for co-transplantation. In the first experiment, the number of Sertoli cell (SC) aggregates and the number of tyrosine hydroxylase positive neurons were compared over time when cell suspensions of Sertoli or ventral mesencephalic cells were kept as a co-suspension mixed at 0 h. Cells from each suspension were dispensed onto glass slides in a manner similar to transplantation. When dispensed in this manner, the number of SC aggregates and TH-positive neurons decreased over 4 h. In experiment 2, the cell suspensions were mixed just prior to injection at each of four timepoints, the number of aggregates and TH neurons was consistent over time. Clearly this latter strategy resulted consistent recovery of both cell types for transplants up to 3 h after suspension preparation.</p>},
  author       = {Othberg, A I and Willing, A E and Saporta, S and Cameron, D F and Sanberg, P R},
  issn         = {0304-3940},
  keyword      = {Animals,Biomarkers,Brain Tissue Transplantation,Cell Aggregation,Cell Culture Techniques,Cell Survival,Cell Transplantation,Coculture Techniques,Fetal Tissue Transplantation,Male,Mesencephalon,Nerve Tissue Proteins,Neurons,Rats,Rats, Sprague-Dawley,Sertoli Cells,Suspensions,Swine,Tyrosine 3-Monooxygenase,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {05},
  number       = {2-3},
  pages        = {4--111},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Preparation of cell suspensions for co-transplantation : methodological considerations},
  url          = {http://dx.doi.org/10.1016/S0304-3940(98)00283-3},
  volume       = {247},
  year         = {1998},
}