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Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coli

Johanson, Urban LU and Hughes, Diarmaid (1992) In Gene 120(1). p.93-98
Abstract

The nucleotide (nt) sequences of the str operon in Escherichia coli K-12 and Salmonella typhimurium LT2 were completed and compared at the nt and amino acid (aa) level. The order of conservation at the nt and aa level is rpsL greater than tufA greater than rpsG greater than f usA. A striking difference is that the rpsG-encoded ribosomal protein, S7, in E. coli K-12 is 23 aa longer than in S. typhimurium. The very low (0.18) codon adaptation index of this part of the E. coli K-12-encoding gene and the unusual stop codon (UGA) suggest that this is a relatively recent extension. A trend towards a higher G+C content in fusA (gene encoding elongation factor (EF)-G) and tufA (gene encoding EF-Tu) in S. typhimurium is noted. In fusA, nt... (More)

The nucleotide (nt) sequences of the str operon in Escherichia coli K-12 and Salmonella typhimurium LT2 were completed and compared at the nt and amino acid (aa) level. The order of conservation at the nt and aa level is rpsL greater than tufA greater than rpsG greater than f usA. A striking difference is that the rpsG-encoded ribosomal protein, S7, in E. coli K-12 is 23 aa longer than in S. typhimurium. The very low (0.18) codon adaptation index of this part of the E. coli K-12-encoding gene and the unusual stop codon (UGA) suggest that this is a relatively recent extension. A trend towards a higher G+C content in fusA (gene encoding elongation factor (EF)-G) and tufA (gene encoding EF-Tu) in S. typhimurium is noted. In fusA, nt substitutions at all three positions in a codon occur at a much higher frequency than expected from the number of nt substitutions in the gene, assuming they are random and independent events. An analysis of substitutions in this and other genes suggests that the triple substitutions in fusA, and some other genes, are the result of the sequential accumulation of individual mutations, probably driven by selection pressure for particular codons or aa.

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published
subject
keywords
Amino Acid Sequence, Bacterial Proteins, Base Sequence, Escherichia coli, Molecular Sequence Data, Operon, Peptide Elongation Factor G, Peptide Elongation Factor Tu, Peptide Elongation Factors, Ribosomal Proteins, Salmonella typhimurium, Sequence Homology, Nucleic Acid, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
in
Gene
volume
120
issue
1
pages
6 pages
publisher
Elsevier
external identifiers
  • Scopus:0026779757
ISSN
0378-1119
DOI
10.1016/0378-1119(92)90014-G
language
English
LU publication?
yes
id
02ee3b41-eed8-4098-854f-138e00930cb4
date added to LUP
2016-10-13 11:43:56
date last changed
2016-11-02 22:55:00
@misc{02ee3b41-eed8-4098-854f-138e00930cb4,
  abstract     = {<p>The nucleotide (nt) sequences of the str operon in Escherichia coli K-12 and Salmonella typhimurium LT2 were completed and compared at the nt and amino acid (aa) level. The order of conservation at the nt and aa level is rpsL greater than tufA greater than rpsG greater than f usA. A striking difference is that the rpsG-encoded ribosomal protein, S7, in E. coli K-12 is 23 aa longer than in S. typhimurium. The very low (0.18) codon adaptation index of this part of the E. coli K-12-encoding gene and the unusual stop codon (UGA) suggest that this is a relatively recent extension. A trend towards a higher G+C content in fusA (gene encoding elongation factor (EF)-G) and tufA (gene encoding EF-Tu) in S. typhimurium is noted. In fusA, nt substitutions at all three positions in a codon occur at a much higher frequency than expected from the number of nt substitutions in the gene, assuming they are random and independent events. An analysis of substitutions in this and other genes suggests that the triple substitutions in fusA, and some other genes, are the result of the sequential accumulation of individual mutations, probably driven by selection pressure for particular codons or aa.</p>},
  author       = {Johanson, Urban and Hughes, Diarmaid},
  issn         = {0378-1119},
  keyword      = {Amino Acid Sequence,Bacterial Proteins,Base Sequence,Escherichia coli,Molecular Sequence Data,Operon,Peptide Elongation Factor G,Peptide Elongation Factor Tu,Peptide Elongation Factors,Ribosomal Proteins,Salmonella typhimurium,Sequence Homology, Nucleic Acid,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {10},
  number       = {1},
  pages        = {93--98},
  publisher    = {ARRAY(0xbeea240)},
  series       = {Gene},
  title        = {Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coli},
  url          = {http://dx.doi.org/10.1016/0378-1119(92)90014-G},
  volume       = {120},
  year         = {1992},
}