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Intracerebral grafting of neuronal cell suspensions. II. Survival and growth of nigral cells implanted in different brain sites

Björklund, Anders LU ; Stenevi, U; Schmidt, R H; Dunnett, S B and Gage, F H (1983) In Acta Physiol. Scand Suppl. 522. p.9-18
Abstract
Dissociated dopamine-rich cell suspensions were prepared from the ventral mesencephalon of rat embryos and injected in one or several sites in striatal and non-striatal regions in the dopaminergically denervated brain of adult rats. While the grafts survived well in all sites, the dopamine fibre outgrowth was markedly different depending on whether the grafts occurred in an area normally innervated by the mesencephalic dopamine neurones (i.e. neostriatum or nc. accumbens) or in areas not normally innervated by these neurones (i.e. parietal cortex, lateral hypothalamus or substantia nigra). Moreover, in grafts placed at different sites along the trajectory of the nigrostriatal pathway the outgrowing fibres remained confined to the graft,... (More)
Dissociated dopamine-rich cell suspensions were prepared from the ventral mesencephalon of rat embryos and injected in one or several sites in striatal and non-striatal regions in the dopaminergically denervated brain of adult rats. While the grafts survived well in all sites, the dopamine fibre outgrowth was markedly different depending on whether the grafts occurred in an area normally innervated by the mesencephalic dopamine neurones (i.e. neostriatum or nc. accumbens) or in areas not normally innervated by these neurones (i.e. parietal cortex, lateral hypothalamus or substantia nigra). Moreover, in grafts placed at different sites along the trajectory of the nigrostriatal pathway the outgrowing fibres remained confined to the graft, and there was little evidence that the implanted neurones could elongate their axons along the pathway of the nigrostriatal tract to reach the striatum from a distance. Thus, the intracerebral suspension grafts provided efficient reinnervation of a denervated target only when placed in the immediate vicinity of the target area. The results of multiple graft placements indicate that a relatively complete restoration of a lost innervation should be possible to achieve in large areas of the brain, such as the striatal complex, with the suspension grafting technique. (Less)
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Animals Brain/anatomy & histology Corpus Striatum/surgery Dopamine/metabolism Embryo, Mammalian/cytology Graft Survival Neurons/metabolism Neurons/transplantation* Rats Rats, Inbred Strains Substantia Nigra/surgery Transplantation, Isogeneic
in
Acta Physiol. Scand
volume
Suppl. 522
pages
9 - 18
publisher
Wiley-Blackwell
language
English
LU publication?
yes
id
d7284a9c-4796-4a70-bfd7-bda67d6a0c0f (old id 1030531)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=6586058dopt=Abstract
date added to LUP
2008-02-11 13:29:48
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2016-04-16 11:29:14
@misc{d7284a9c-4796-4a70-bfd7-bda67d6a0c0f,
  abstract     = {Dissociated dopamine-rich cell suspensions were prepared from the ventral mesencephalon of rat embryos and injected in one or several sites in striatal and non-striatal regions in the dopaminergically denervated brain of adult rats. While the grafts survived well in all sites, the dopamine fibre outgrowth was markedly different depending on whether the grafts occurred in an area normally innervated by the mesencephalic dopamine neurones (i.e. neostriatum or nc. accumbens) or in areas not normally innervated by these neurones (i.e. parietal cortex, lateral hypothalamus or substantia nigra). Moreover, in grafts placed at different sites along the trajectory of the nigrostriatal pathway the outgrowing fibres remained confined to the graft, and there was little evidence that the implanted neurones could elongate their axons along the pathway of the nigrostriatal tract to reach the striatum from a distance. Thus, the intracerebral suspension grafts provided efficient reinnervation of a denervated target only when placed in the immediate vicinity of the target area. The results of multiple graft placements indicate that a relatively complete restoration of a lost innervation should be possible to achieve in large areas of the brain, such as the striatal complex, with the suspension grafting technique.},
  author       = {Björklund, Anders and Stenevi, U and Schmidt, R H and Dunnett, S B and Gage, F H},
  keyword      = {Animals
Brain/anatomy & histology
Corpus Striatum/surgery
Dopamine/metabolism
Embryo,Mammalian/cytology
Graft Survival
Neurons/metabolism
Neurons/transplantation*
Rats
Rats,Inbred Strains
Substantia Nigra/surgery
Transplantation,Isogeneic},
  language     = {eng},
  pages        = {9--18},
  publisher    = {ARRAY(0x8ecd3f0)},
  series       = {Acta Physiol. Scand},
  title        = {Intracerebral grafting of neuronal cell suspensions. II. Survival and growth of nigral cells implanted in different brain sites},
  volume       = {Suppl. 522},
  year         = {1983},
}