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Intracerebral grafting of neuronal cell suspensions. VI. Survival and growth of intrahippocampal implants of septal cell suspensions

Björklund, Anders LU ; Gage, F H; Stenevi, U and Dunnett, S B (1983) In Acta Physiol.Scand Suppl. 522. p.49-58
Abstract
The survival and growth of intrahippocampal septal suspension grafts were investigated by acetylcholine esterase (AChE) histochemistry in animals with lesions of the intrinsic septohippocampal cholinergic pathways. AChE was demonstrable in the grafts after the first postoperative week, and AChE-positive fibres were seen to extend into the host hippocampus by 3 weeks. Rapid fibre outgrowth occurred between 3 weeks and 3 months after grafting, and continued at a slower rate thereafter. By 6 months a fairly complete reinnervation of the initially denervated hippocampus was achieved in most specimens, and this persisted at 14 months, the longest postoperative time analysed. A comparison between the development of the AChE-positive neurones in... (More)
The survival and growth of intrahippocampal septal suspension grafts were investigated by acetylcholine esterase (AChE) histochemistry in animals with lesions of the intrinsic septohippocampal cholinergic pathways. AChE was demonstrable in the grafts after the first postoperative week, and AChE-positive fibres were seen to extend into the host hippocampus by 3 weeks. Rapid fibre outgrowth occurred between 3 weeks and 3 months after grafting, and continued at a slower rate thereafter. By 6 months a fairly complete reinnervation of the initially denervated hippocampus was achieved in most specimens, and this persisted at 14 months, the longest postoperative time analysed. A comparison between the development of the AChE-positive neurones in the suspension grafts with that seen during ontogeny in situ suggested that the grafted neurones lagged behind normal development by at least 1 week. Similar to our previous observations on septal grafts implanted as solid tissue pieces, the pattern of the newly-formed AChE-positive innervation in the host hippocampal formation, established from the septal suspension grafts, was remarkably similar to that of the normal AChE-positive septal innervation. This pattern became established as soon as the graft-derived fibres first grew in, suggesting that the ingrowing axons extended and ramified preferentially into those hippocampal subfields which normally receive an AChE-positive innervation from the septal-diagonal band area. (Less)
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keywords
Inbred Strains Septum Pellucidum/transplantation* Time Factors Transplantation, Isogeneic Acetylcholinesterase, Acetylcholinesterase/metabolism Animals Embryo, Mammalian/cytology Female Graft Survival Hippocampus/anatomy & histology Hippocampus/surgery Neurons/enzymology Neurons/transplantation* Rats Rats
in
Acta Physiol.Scand
volume
Suppl. 522
pages
49 - 58
publisher
Wiley-Blackwell
language
English
LU publication?
yes
id
35be7989-d109-48da-9504-c35dec6327d6 (old id 1030582)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=6586055dopt=Abstract
date added to LUP
2008-02-11 13:29:54
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2016-04-16 12:00:33
@misc{35be7989-d109-48da-9504-c35dec6327d6,
  abstract     = {The survival and growth of intrahippocampal septal suspension grafts were investigated by acetylcholine esterase (AChE) histochemistry in animals with lesions of the intrinsic septohippocampal cholinergic pathways. AChE was demonstrable in the grafts after the first postoperative week, and AChE-positive fibres were seen to extend into the host hippocampus by 3 weeks. Rapid fibre outgrowth occurred between 3 weeks and 3 months after grafting, and continued at a slower rate thereafter. By 6 months a fairly complete reinnervation of the initially denervated hippocampus was achieved in most specimens, and this persisted at 14 months, the longest postoperative time analysed. A comparison between the development of the AChE-positive neurones in the suspension grafts with that seen during ontogeny in situ suggested that the grafted neurones lagged behind normal development by at least 1 week. Similar to our previous observations on septal grafts implanted as solid tissue pieces, the pattern of the newly-formed AChE-positive innervation in the host hippocampal formation, established from the septal suspension grafts, was remarkably similar to that of the normal AChE-positive septal innervation. This pattern became established as soon as the graft-derived fibres first grew in, suggesting that the ingrowing axons extended and ramified preferentially into those hippocampal subfields which normally receive an AChE-positive innervation from the septal-diagonal band area.},
  author       = {Björklund, Anders and Gage, F H and Stenevi, U and Dunnett, S B},
  keyword      = {Inbred Strains
Septum Pellucidum/transplantation*
Time Factors
Transplantation,Isogeneic
Acetylcholinesterase,Acetylcholinesterase/metabolism
Animals
Embryo,Mammalian/cytology
Female
Graft Survival
Hippocampus/anatomy & histology
Hippocampus/surgery
Neurons/enzymology
Neurons/transplantation*
Rats
Rats},
  language     = {eng},
  pages        = {49--58},
  publisher    = {ARRAY(0x96a0120)},
  series       = {Acta Physiol.Scand},
  title        = {Intracerebral grafting of neuronal cell suspensions. VI. Survival and growth of intrahippocampal implants of septal cell suspensions},
  volume       = {Suppl. 522},
  year         = {1983},
}