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Intracerebral grafting of neuronal cell suspensions. VIII. Cell survival and axonal outgrcwth of dcpaminergic and cholinergic cells in the aged brain.

Gage, F H; Björklund, Anders LU ; Stenevi, U and Dunnett, S B (1983) In Acta Physiol. Scand. Suppl. 522. p.67-75
Abstract
Neuronal cell suspensions prepared from the ventral mesencephalon and the septal-diagonal band area of rat embryos were implanted into the depth of the intact neostriatum or hippocampus of 21-23 month old female rats. Graft survival, assessed 3-4 months after grafting, was comparable to that seen in our previous studies of young adult recipients. Fibre outgrowth into the host brain was evaluated in animals which were subjected to lesions of the intrinsic nigrostriatal or septohippocampal system 6-10 days before killing. Dense dopamine fibre outgrowth was seen within a zone of up to about 1 mm radius around the nigral implants, and dense growth of acetylcholine esterase (AChE) positive fibres occurred up to about 2 mm away from the septal... (More)
Neuronal cell suspensions prepared from the ventral mesencephalon and the septal-diagonal band area of rat embryos were implanted into the depth of the intact neostriatum or hippocampus of 21-23 month old female rats. Graft survival, assessed 3-4 months after grafting, was comparable to that seen in our previous studies of young adult recipients. Fibre outgrowth into the host brain was evaluated in animals which were subjected to lesions of the intrinsic nigrostriatal or septohippocampal system 6-10 days before killing. Dense dopamine fibre outgrowth was seen within a zone of up to about 1 mm radius around the nigral implants, and dense growth of acetylcholine esterase (AChE) positive fibres occurred up to about 2 mm away from the septal implants. The overall magnitude of fibre outgrowth was less than that generally seen in previously denervated targets in young adult recipients, but it appeared to be as extensive as in young recipients when the grafts are placed in non-denervated targets. The distribution of the AChE-positive fibres from the septal implants in the host hippocampus suggested that the pattern found in the non-denervated target of the aged recipients was more diffuse, and partly different, from normal, and that age-dependent synapse loss in intrinsic connections may influence the patterning of the graft-derived innervation. (Less)
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Mammalian/cytology Female Graft Survival Neurons/metabolism Neurons/transplantation* Rats Rats, Acetylcholinesterase/metabolism Aging Animals Brain/surgery Embryo, Inbred Strains Septum Pellucidum/transplantation* Substantia Nigra/transplantation* Transplantation, Isogeneic Acetylcholinesterase
in
Acta Physiol. Scand.
volume
Suppl. 522
pages
67 - 75
publisher
Wiley-Blackwell
language
English
LU publication?
yes
id
ff7a550a-ce15-4e70-9849-2a0a24235227 (old id 1030596)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=6586057dopt=Abstract
date added to LUP
2008-02-11 13:30:21
date last changed
2016-04-16 11:26:13
@misc{ff7a550a-ce15-4e70-9849-2a0a24235227,
  abstract     = {Neuronal cell suspensions prepared from the ventral mesencephalon and the septal-diagonal band area of rat embryos were implanted into the depth of the intact neostriatum or hippocampus of 21-23 month old female rats. Graft survival, assessed 3-4 months after grafting, was comparable to that seen in our previous studies of young adult recipients. Fibre outgrowth into the host brain was evaluated in animals which were subjected to lesions of the intrinsic nigrostriatal or septohippocampal system 6-10 days before killing. Dense dopamine fibre outgrowth was seen within a zone of up to about 1 mm radius around the nigral implants, and dense growth of acetylcholine esterase (AChE) positive fibres occurred up to about 2 mm away from the septal implants. The overall magnitude of fibre outgrowth was less than that generally seen in previously denervated targets in young adult recipients, but it appeared to be as extensive as in young recipients when the grafts are placed in non-denervated targets. The distribution of the AChE-positive fibres from the septal implants in the host hippocampus suggested that the pattern found in the non-denervated target of the aged recipients was more diffuse, and partly different, from normal, and that age-dependent synapse loss in intrinsic connections may influence the patterning of the graft-derived innervation.},
  author       = {Gage, F H and Björklund, Anders and Stenevi, U and Dunnett, S B},
  keyword      = {Mammalian/cytology
Female
Graft Survival
Neurons/metabolism
Neurons/transplantation*
Rats
Rats,Acetylcholinesterase/metabolism
Aging
Animals
Brain/surgery
Embryo,Inbred Strains
Septum Pellucidum/transplantation*
Substantia Nigra/transplantation*
Transplantation,Isogeneic
Acetylcholinesterase},
  language     = {eng},
  pages        = {67--75},
  publisher    = {ARRAY(0xa7a2c58)},
  series       = {Acta Physiol. Scand.},
  title        = {Intracerebral grafting of neuronal cell suspensions. VIII. Cell survival and axonal outgrcwth of dcpaminergic and cholinergic cells in the aged brain.},
  volume       = {Suppl. 522},
  year         = {1983},
}