Nanomolar concentrations of lysophosphatidylcholine recruit monocytes and induce pro-inflammatory cytokine production in macrophages.
Berg, Katarina LU ; Andersson, Linda LU ; Nilsson, Jan LU and Björkbacka, Harry LU
(2008)
In Biochemical and Biophysical Research Communications
370.
p.348-352
- Abstract
- Lysophosphatidylcholine (LPC) has been attributed a pro-inflammatory role in atherosclerosis. Cell culture studies have identified stimulation of cytokine expression and chemotaxis by micromolar (muM) concentrations of LPC. In the present study we have investigated if LPC, in similarity with many other lipid mediators, has pro-inflammatory effects also at nanomolar (nM) concentrations. Cultured mouse bone marrow derived and RAW264.7 macrophages exposed to LPC demonstrated two peaks of increased MIP-2 release and mRNA expression; one at 0.1-10nM and another at muM concentrations. Both concentration ranges of LPC were also found to stimulate THP-1 monocyte chemotaxis. However, stimulation of the cells with muM concentrations of LPC may cause... (More)
- Lysophosphatidylcholine (LPC) has been attributed a pro-inflammatory role in atherosclerosis. Cell culture studies have identified stimulation of cytokine expression and chemotaxis by micromolar (muM) concentrations of LPC. In the present study we have investigated if LPC, in similarity with many other lipid mediators, has pro-inflammatory effects also at nanomolar (nM) concentrations. Cultured mouse bone marrow derived and RAW264.7 macrophages exposed to LPC demonstrated two peaks of increased MIP-2 release and mRNA expression; one at 0.1-10nM and another at muM concentrations. Both concentration ranges of LPC were also found to stimulate THP-1 monocyte chemotaxis. However, stimulation of the cells with muM concentrations of LPC may cause cell injury as increased release of lactate dehydrogenase was observed. Our findings demonstrate two peaks of LPC-induced pro-inflammatory activity, one in the nM and one in the muM range, and indicate that the latter may involve a stress response to lipid cytotoxicity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1052073
- author
- Berg, Katarina
LU
; Andersson, Linda
LU
; Nilsson, Jan
LU
and Björkbacka, Harry
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical and Biophysical Research Communications
- volume
- 370
- pages
- 348 - 352
- publisher
- Elsevier
- external identifiers
-
- pmid:18371300
- wos:000255526300029
- scopus:42249091172
- pmid:18371300
- ISSN
- 1090-2104
- DOI
- 10.1016/j.bbrc.2008.03.087
- language
- English
- LU publication?
- yes
- id
- 88e2f50d-e7f8-40de-9c37-2f87664ed0d5 (old id 1052073)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18371300?dopt=Abstract
- date added to LUP
- 2016-04-04 09:24:42
- date last changed
- 2022-04-23 20:23:52
@article{88e2f50d-e7f8-40de-9c37-2f87664ed0d5,
abstract = {{Lysophosphatidylcholine (LPC) has been attributed a pro-inflammatory role in atherosclerosis. Cell culture studies have identified stimulation of cytokine expression and chemotaxis by micromolar (muM) concentrations of LPC. In the present study we have investigated if LPC, in similarity with many other lipid mediators, has pro-inflammatory effects also at nanomolar (nM) concentrations. Cultured mouse bone marrow derived and RAW264.7 macrophages exposed to LPC demonstrated two peaks of increased MIP-2 release and mRNA expression; one at 0.1-10nM and another at muM concentrations. Both concentration ranges of LPC were also found to stimulate THP-1 monocyte chemotaxis. However, stimulation of the cells with muM concentrations of LPC may cause cell injury as increased release of lactate dehydrogenase was observed. Our findings demonstrate two peaks of LPC-induced pro-inflammatory activity, one in the nM and one in the muM range, and indicate that the latter may involve a stress response to lipid cytotoxicity.}},
author = {{Berg, Katarina and Andersson, Linda and Nilsson, Jan and Björkbacka, Harry}},
issn = {{1090-2104}},
language = {{eng}},
pages = {{348--352}},
publisher = {{Elsevier}},
series = {{Biochemical and Biophysical Research Communications}},
title = {{Nanomolar concentrations of lysophosphatidylcholine recruit monocytes and induce pro-inflammatory cytokine production in macrophages.}},
url = {{http://dx.doi.org/10.1016/j.bbrc.2008.03.087}},
doi = {{10.1016/j.bbrc.2008.03.087}},
volume = {{370}},
year = {{2008}},
}