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Impaired wound healing in an acute diabetic pig model and the effects of local hyperglycemia.

Velander, Patrik LU ; Theopold, Christoph; Hirsch, Tobias; Bleiziffer, Oliver; Zuhaili, Baraa; Fossum, Magdalena; Hoeller, Daniela; Gheerardyn, Raphael; Chen, Michael and Visovatti, Scott, et al. (2008) In Wound Repair and Regeneration 16(2). p.288-293
Abstract
Diabetic wounds result in significant morbidity, prolonged hospitalization, and enormous health-care expenses. Pigs have been shown to have wound healing resembling that in humans. The aim of this study was to develop a large-animal model for diabetic wound healing. Diabetes was induced by streptozotocin injection in Yorkshire pigs. Full-thickness wounds were created and dressed with a sealed chamber. Nondiabetic pigs with or without high glucose wound fluid concentration served as controls. Glucose concentration in serum and wound fluid was measured and collected. Wound contraction was monitored, and biopsies were obtained for measurement of reepithelialization. Wound fluid was analyzed for insulin-like growth factor-1 (IGF-1),... (More)
Diabetic wounds result in significant morbidity, prolonged hospitalization, and enormous health-care expenses. Pigs have been shown to have wound healing resembling that in humans. The aim of this study was to develop a large-animal model for diabetic wound healing. Diabetes was induced by streptozotocin injection in Yorkshire pigs. Full-thickness wounds were created and dressed with a sealed chamber. Nondiabetic pigs with or without high glucose wound fluid concentration served as controls. Glucose concentration in serum and wound fluid was measured and collected. Wound contraction was monitored, and biopsies were obtained for measurement of reepithelialization. Wound fluid was analyzed for insulin-like growth factor-1 (IGF-1), platelet-derived growth factor, and transforming growth factor. Glucose concentration in wound fluid initially followed serum levels and then decreased to undetectable on day 9. Reepithelialization was significantly delayed in diabetic pigs. In nondiabetic pigs, wounds treated in a local hyperglycemic environment, and thus excluding the effects of systemic hyperglycemia, showed no difference in wound closure compared with controls. This suggests that delayed wound healing in diabetes is not induced by local high-glucose concentration itself. Analysis of growth factor expression showed a marked reduction in IGF-1 in the diabetic wounds. Diabetic pigs have impaired healing that is accompanied by a reduction of IGF-1 in the healing wound and is not due to the local hyperglycemia condition itself. (Less)
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Contribution to journal
publication status
published
subject
in
Wound Repair and Regeneration
volume
16
issue
2
pages
288 - 293
publisher
Wiley-Blackwell
external identifiers
  • PMID:18318812
  • WOS:000253761000018
  • Scopus:40349108869
ISSN
1524-475X
DOI
10.1111/j.1524-475X.2008.00367.x
language
English
LU publication?
yes
id
9a6f8fc6-9f51-4d18-bdea-10c4af08de37 (old id 1052813)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18318812?dopt=Abstract
date added to LUP
2008-04-03 08:13:42
date last changed
2016-10-23 04:30:27
@misc{9a6f8fc6-9f51-4d18-bdea-10c4af08de37,
  abstract     = {Diabetic wounds result in significant morbidity, prolonged hospitalization, and enormous health-care expenses. Pigs have been shown to have wound healing resembling that in humans. The aim of this study was to develop a large-animal model for diabetic wound healing. Diabetes was induced by streptozotocin injection in Yorkshire pigs. Full-thickness wounds were created and dressed with a sealed chamber. Nondiabetic pigs with or without high glucose wound fluid concentration served as controls. Glucose concentration in serum and wound fluid was measured and collected. Wound contraction was monitored, and biopsies were obtained for measurement of reepithelialization. Wound fluid was analyzed for insulin-like growth factor-1 (IGF-1), platelet-derived growth factor, and transforming growth factor. Glucose concentration in wound fluid initially followed serum levels and then decreased to undetectable on day 9. Reepithelialization was significantly delayed in diabetic pigs. In nondiabetic pigs, wounds treated in a local hyperglycemic environment, and thus excluding the effects of systemic hyperglycemia, showed no difference in wound closure compared with controls. This suggests that delayed wound healing in diabetes is not induced by local high-glucose concentration itself. Analysis of growth factor expression showed a marked reduction in IGF-1 in the diabetic wounds. Diabetic pigs have impaired healing that is accompanied by a reduction of IGF-1 in the healing wound and is not due to the local hyperglycemia condition itself.},
  author       = {Velander, Patrik and Theopold, Christoph and Hirsch, Tobias and Bleiziffer, Oliver and Zuhaili, Baraa and Fossum, Magdalena and Hoeller, Daniela and Gheerardyn, Raphael and Chen, Michael and Visovatti, Scott and Svensson, Henry and Yao, Feng and Eriksson, Elof},
  issn         = {1524-475X},
  language     = {eng},
  number       = {2},
  pages        = {288--293},
  publisher    = {ARRAY(0x69b0f48)},
  series       = {Wound Repair and Regeneration},
  title        = {Impaired wound healing in an acute diabetic pig model and the effects of local hyperglycemia.},
  url          = {http://dx.doi.org/10.1111/j.1524-475X.2008.00367.x},
  volume       = {16},
  year         = {2008},
}