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Microwave-assisted rapid characterization of lipase selectivities.

Bradoo, Sapna LU ; Rathi, Pooja; Saxena, R K and Gupta, Rani (2002) In Journal of Biochemical and Biophysical Methods 51(2). p.115-120
Abstract
A rapid screening procedure for characterization of lipase selectivities using microwaves was developed. The rate of reaction of various commercial lipases (porcine pancreas, Mucor miehei, Candida rugosa, Pseudomonas cepacia) as well as lipases from laboratory isolates-Bacillus stearothermophilus and Burkholderia cepacia RGP-10 for triolein hydrolysis was 7- to 12-fold higher in a microwave oven as compared to that by pH stat. The esterification of sucrose/methanol and ascorbic acid with different fatty acids was also achieved within 30 s in a microwave using porcine pancreas, B. stearothermophilus SB-1 and B. cepacia RGP-10 lipases. The relative rates and selectivity of the lipases both for hydrolytic and synthesis reactions remains... (More)
A rapid screening procedure for characterization of lipase selectivities using microwaves was developed. The rate of reaction of various commercial lipases (porcine pancreas, Mucor miehei, Candida rugosa, Pseudomonas cepacia) as well as lipases from laboratory isolates-Bacillus stearothermophilus and Burkholderia cepacia RGP-10 for triolein hydrolysis was 7- to 12-fold higher in a microwave oven as compared to that by pH stat. The esterification of sucrose/methanol and ascorbic acid with different fatty acids was also achieved within 30 s in a microwave using porcine pancreas, B. stearothermophilus SB-1 and B. cepacia RGP-10 lipases. The relative rates and selectivity of the lipases both for hydrolytic and synthesis reactions remains unaltered. However, the rate of reaction was dynamically enhanced when exposed to microwaves. Microwave-assisted enzyme catalysis can become an attractive procedure for rapid characterization of large number of enzyme samples and substrates, which otherwise is a cumbersome and time-consuming exercise. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Microwaves, Lipase : metabolism, Lipase : chemistry, Hydrolysis, Hydrogen-Ion Concentration, Biochemistry : methods, Bacteria : enzymology, Ascorbic Acid : chemistry, Animal, Time Factors, Triglycerides : chemistry, Substrate Specificity, Sucrose : chemistry, Support, Non-U.S. Gov't, Swine
in
Journal of Biochemical and Biophysical Methods
volume
51
issue
2
pages
115 - 120
publisher
Elsevier
external identifiers
  • WOS:000175641700003
  • PMID:12062110
  • Scopus:0037129173
ISSN
0165-022X
DOI
10.1016/S0165-022X(02)00005-2
language
English
LU publication?
yes
id
4b4806a2-233b-40ff-8434-084fa1252539 (old id 108798)
date added to LUP
2007-07-02 08:55:03
date last changed
2016-10-13 04:26:05
@misc{4b4806a2-233b-40ff-8434-084fa1252539,
  abstract     = {A rapid screening procedure for characterization of lipase selectivities using microwaves was developed. The rate of reaction of various commercial lipases (porcine pancreas, Mucor miehei, Candida rugosa, Pseudomonas cepacia) as well as lipases from laboratory isolates-Bacillus stearothermophilus and Burkholderia cepacia RGP-10 for triolein hydrolysis was 7- to 12-fold higher in a microwave oven as compared to that by pH stat. The esterification of sucrose/methanol and ascorbic acid with different fatty acids was also achieved within 30 s in a microwave using porcine pancreas, B. stearothermophilus SB-1 and B. cepacia RGP-10 lipases. The relative rates and selectivity of the lipases both for hydrolytic and synthesis reactions remains unaltered. However, the rate of reaction was dynamically enhanced when exposed to microwaves. Microwave-assisted enzyme catalysis can become an attractive procedure for rapid characterization of large number of enzyme samples and substrates, which otherwise is a cumbersome and time-consuming exercise.},
  author       = {Bradoo, Sapna and Rathi, Pooja and Saxena, R K and Gupta, Rani},
  issn         = {0165-022X},
  keyword      = {Microwaves,Lipase : metabolism,Lipase : chemistry,Hydrolysis,Hydrogen-Ion Concentration,Biochemistry : methods,Bacteria : enzymology,Ascorbic Acid : chemistry,Animal,Time Factors,Triglycerides : chemistry,Substrate Specificity,Sucrose : chemistry,Support,Non-U.S. Gov't,Swine},
  language     = {eng},
  number       = {2},
  pages        = {115--120},
  publisher    = {ARRAY(0xa1bd0d8)},
  series       = {Journal of Biochemical and Biophysical Methods},
  title        = {Microwave-assisted rapid characterization of lipase selectivities.},
  url          = {http://dx.doi.org/10.1016/S0165-022X(02)00005-2},
  volume       = {51},
  year         = {2002},
}