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Hematopoietic reconstitution by multipotent adult progenitor cells: precursors to long-term hematopoietic stem cells

Serafini, Marta; Dylla, Scott J; Oki, Masayuki; Heremans, Yves; Tolar, Jakub; Jiang, Yuehua; Buckley, Shannon M; Pelacho, Beatriz; Burns, Terry C and Frommer, Sarah, et al. (2007) In Journal of Experimental Medicine 204(1). p.129-139
Abstract
For decades, in vitro expansion of transplantable hematopoietic stem cells (HSCs) has been an elusive goal. Here, we demonstrate that multipotent adult progenitor cells (MAPCs), isolated from green fluorescent protein (GFP)-transgenic mice and expanded in vitro for >40-80 population doublings, are capable of multilineage hematopoietic engraftment of immunodeficient mice. Among MAPC-derived GFP+CD45.2+ cells in the bone marrow of engrafted mice, HSCs were present that could radioprotect and reconstitute multilineage hematopoiesis in secondary and tertiary recipients, as well as myeloid and lymphoid hematopoietic progenitor subsets and functional GFP+ MAPC-derived lymphocytes that were functional. Although hematopoietic contribution by... (More)
For decades, in vitro expansion of transplantable hematopoietic stem cells (HSCs) has been an elusive goal. Here, we demonstrate that multipotent adult progenitor cells (MAPCs), isolated from green fluorescent protein (GFP)-transgenic mice and expanded in vitro for >40-80 population doublings, are capable of multilineage hematopoietic engraftment of immunodeficient mice. Among MAPC-derived GFP+CD45.2+ cells in the bone marrow of engrafted mice, HSCs were present that could radioprotect and reconstitute multilineage hematopoiesis in secondary and tertiary recipients, as well as myeloid and lymphoid hematopoietic progenitor subsets and functional GFP+ MAPC-derived lymphocytes that were functional. Although hematopoietic contribution by MAPCs was comparable to control KTLS HSCs, approximately 10(3)-fold more MAPCs were required for efficient engraftment. Because GFP+ host-derived CD45.1+ cells were not observed, fusion is not likely to account for the generation of HSCs by MAPCs. (Less)
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published
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Journal of Experimental Medicine
volume
204
issue
1
pages
129 - 139
publisher
Rockefeller University Press
external identifiers
  • PMID:17227908
  • Scopus:33846437812
ISSN
1540-9538
DOI
10.1084/jem.20061115
language
English
LU publication?
yes
id
0e1ee5dc-6c5c-400f-af82-ebda69133bfb (old id 1140371)
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2008-08-15 11:21:40
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2016-10-13 04:32:37
@misc{0e1ee5dc-6c5c-400f-af82-ebda69133bfb,
  abstract     = {For decades, in vitro expansion of transplantable hematopoietic stem cells (HSCs) has been an elusive goal. Here, we demonstrate that multipotent adult progenitor cells (MAPCs), isolated from green fluorescent protein (GFP)-transgenic mice and expanded in vitro for >40-80 population doublings, are capable of multilineage hematopoietic engraftment of immunodeficient mice. Among MAPC-derived GFP+CD45.2+ cells in the bone marrow of engrafted mice, HSCs were present that could radioprotect and reconstitute multilineage hematopoiesis in secondary and tertiary recipients, as well as myeloid and lymphoid hematopoietic progenitor subsets and functional GFP+ MAPC-derived lymphocytes that were functional. Although hematopoietic contribution by MAPCs was comparable to control KTLS HSCs, approximately 10(3)-fold more MAPCs were required for efficient engraftment. Because GFP+ host-derived CD45.1+ cells were not observed, fusion is not likely to account for the generation of HSCs by MAPCs.},
  author       = {Serafini, Marta and Dylla, Scott J and Oki, Masayuki and Heremans, Yves and Tolar, Jakub and Jiang, Yuehua and Buckley, Shannon M and Pelacho, Beatriz and Burns, Terry C and Frommer, Sarah and Rossi, Derrick J and Bryder, David and Panoskaltsis-Mortari, Angela and O'Shaughnessy, Matt},
  issn         = {1540-9538},
  language     = {eng},
  number       = {1},
  pages        = {129--139},
  publisher    = {ARRAY(0xb529380)},
  series       = {Journal of Experimental Medicine},
  title        = {Hematopoietic reconstitution by multipotent adult progenitor cells: precursors to long-term hematopoietic stem cells},
  url          = {http://dx.doi.org/10.1084/jem.20061115},
  volume       = {204},
  year         = {2007},
}