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A new mutation in 16S rRNA of Escherichia coli conferring spectinomycin resistance

Johanson, U LU and Hughes, D (1995) In Nucleic Acids Research 23(3). p.6-464
Abstract

We report a novel mutation, C1066U in 16S rRNA which was selected for resistance to spectinomycin, an antibiotic which inhibits ribosomal translocation. The minimal inhibitory concentration (MIC) of spectinomycin determined for this mutant (15 micrograms/ml) is greater than with the wild-type plasmid (5 micrograms/ml) but lower than with the well known C1192U mutation (> 80 micrograms/ml). The C1066U mutation also increases the cells sensitivity to fusidic acid, another antibiotic which inhibits translation at the translocation stage, whereas C1192U is unchanged relative to the wild type. We discuss why the acquisition of resistance to one of these drugs is often associated with hypersensitivity to the other.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Base Sequence, DNA Mutational Analysis, Drug Resistance, Microbial, Escherichia coli, Fusidic Acid, Microbial Sensitivity Tests, Molecular Sequence Data, Nucleic Acid Conformation, Point Mutation, Polyribosomes, RNA, Ribosomal, 16S, Spectinomycin, Journal Article, Research Support, Non-U.S. Gov't
in
Nucleic Acids Research
volume
23
issue
3
pages
3 pages
publisher
Oxford University Press
external identifiers
  • Scopus:0028944493
ISSN
0305-1048
language
English
LU publication?
yes
id
11448813-4ec9-4dee-aff1-1410de0ee00f
alternative location
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC306698/
date added to LUP
2016-10-13 11:51:20
date last changed
2016-11-02 23:10:33
@misc{11448813-4ec9-4dee-aff1-1410de0ee00f,
  abstract     = {<p>We report a novel mutation, C1066U in 16S rRNA which was selected for resistance to spectinomycin, an antibiotic which inhibits ribosomal translocation. The minimal inhibitory concentration (MIC) of spectinomycin determined for this mutant (15 micrograms/ml) is greater than with the wild-type plasmid (5 micrograms/ml) but lower than with the well known C1192U mutation (&gt; 80 micrograms/ml). The C1066U mutation also increases the cells sensitivity to fusidic acid, another antibiotic which inhibits translation at the translocation stage, whereas C1192U is unchanged relative to the wild type. We discuss why the acquisition of resistance to one of these drugs is often associated with hypersensitivity to the other.</p>},
  author       = {Johanson, U and Hughes, D},
  issn         = {0305-1048},
  keyword      = {Base Sequence,DNA Mutational Analysis,Drug Resistance, Microbial,Escherichia coli,Fusidic Acid,Microbial Sensitivity Tests,Molecular Sequence Data,Nucleic Acid Conformation,Point Mutation,Polyribosomes,RNA, Ribosomal, 16S,Spectinomycin,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {02},
  number       = {3},
  pages        = {6--464},
  publisher    = {ARRAY(0xdaec788)},
  series       = {Nucleic Acids Research},
  title        = {A new mutation in 16S rRNA of Escherichia coli conferring spectinomycin resistance},
  volume       = {23},
  year         = {1995},
}