A new mutation in 16S rRNA of Escherichia coli conferring spectinomycin resistance
(1995) In Nucleic Acids Research 23(3). p.6-464- Abstract
We report a novel mutation, C1066U in 16S rRNA which was selected for resistance to spectinomycin, an antibiotic which inhibits ribosomal translocation. The minimal inhibitory concentration (MIC) of spectinomycin determined for this mutant (15 micrograms/ml) is greater than with the wild-type plasmid (5 micrograms/ml) but lower than with the well known C1192U mutation (> 80 micrograms/ml). The C1066U mutation also increases the cells sensitivity to fusidic acid, another antibiotic which inhibits translation at the translocation stage, whereas C1192U is unchanged relative to the wild type. We discuss why the acquisition of resistance to one of these drugs is often associated with hypersensitivity to the other.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/11448813-4ec9-4dee-aff1-1410de0ee00f
- author
- Johanson, U LU and Hughes, D
- organization
- publishing date
- 1995-02-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Base Sequence, DNA Mutational Analysis, Drug Resistance, Microbial, Escherichia coli, Fusidic Acid, Microbial Sensitivity Tests, Molecular Sequence Data, Nucleic Acid Conformation, Point Mutation, Polyribosomes, RNA, Ribosomal, 16S, Spectinomycin, Journal Article, Research Support, Non-U.S. Gov't
- in
- Nucleic Acids Research
- volume
- 23
- issue
- 3
- pages
- 3 pages
- publisher
- Oxford University Press
- external identifiers
-
- scopus:0028944493
- pmid:7885842
- ISSN
- 0305-1048
- language
- English
- LU publication?
- yes
- id
- 11448813-4ec9-4dee-aff1-1410de0ee00f
- alternative location
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC306698/
- date added to LUP
- 2016-10-13 11:51:20
- date last changed
- 2024-01-31 02:49:58
@article{11448813-4ec9-4dee-aff1-1410de0ee00f, abstract = {{<p>We report a novel mutation, C1066U in 16S rRNA which was selected for resistance to spectinomycin, an antibiotic which inhibits ribosomal translocation. The minimal inhibitory concentration (MIC) of spectinomycin determined for this mutant (15 micrograms/ml) is greater than with the wild-type plasmid (5 micrograms/ml) but lower than with the well known C1192U mutation (> 80 micrograms/ml). The C1066U mutation also increases the cells sensitivity to fusidic acid, another antibiotic which inhibits translation at the translocation stage, whereas C1192U is unchanged relative to the wild type. We discuss why the acquisition of resistance to one of these drugs is often associated with hypersensitivity to the other.</p>}}, author = {{Johanson, U and Hughes, D}}, issn = {{0305-1048}}, keywords = {{Base Sequence; DNA Mutational Analysis; Drug Resistance, Microbial; Escherichia coli; Fusidic Acid; Microbial Sensitivity Tests; Molecular Sequence Data; Nucleic Acid Conformation; Point Mutation; Polyribosomes; RNA, Ribosomal, 16S; Spectinomycin; Journal Article; Research Support, Non-U.S. Gov't}}, language = {{eng}}, month = {{02}}, number = {{3}}, pages = {{6--464}}, publisher = {{Oxford University Press}}, series = {{Nucleic Acids Research}}, title = {{A new mutation in 16S rRNA of Escherichia coli conferring spectinomycin resistance}}, url = {{https://www.ncbi.nlm.nih.gov/pmc/articles/PMC306698/}}, volume = {{23}}, year = {{1995}}, }