Advanced

Inflammatory markers in nondiabetic and diabetic rat retinas exposed to ischemia followed by reperfusion.

Gustavsson, Carin LU ; Agardh, Carl-David LU ; Hagert, Per LU and Agardh, Elisabet LU (2008) In Retina 28(4). p.645-652
Abstract
PURPOSE:: To examine the retinal inflammatory response to ischemia-reperfusion in nondiabetic and diabetic rats injected with either an omega-3-polyunsaturated fatty acid (docosahexaenoic acid [DHA]) or a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor (pravastatin). METHODS:: Diabetes was induced by an intraperitoneal injection of streptozocin, and retinal ischemia was induced by ligation of the optic nerve and vessels, followed by reperfusion for 1 hour or 24 hours. Five minutes before surgery, an intravenous injection of DHA, pravastatin, or vehicle (ethanol) was administered. The mRNA expressions of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, caspase-1, IL-1beta, P-selectin, vascular cellular adhesion molecule... (More)
PURPOSE:: To examine the retinal inflammatory response to ischemia-reperfusion in nondiabetic and diabetic rats injected with either an omega-3-polyunsaturated fatty acid (docosahexaenoic acid [DHA]) or a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor (pravastatin). METHODS:: Diabetes was induced by an intraperitoneal injection of streptozocin, and retinal ischemia was induced by ligation of the optic nerve and vessels, followed by reperfusion for 1 hour or 24 hours. Five minutes before surgery, an intravenous injection of DHA, pravastatin, or vehicle (ethanol) was administered. The mRNA expressions of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, caspase-1, IL-1beta, P-selectin, vascular cellular adhesion molecule (VCAM)-1, and intercellular adhesion molecule (ICAM)-1 were compared between ischemic and nonischemic retinas as well as diabetic and nondiabetic nonischemic retinas. RESULTS:: Ischemia induced increased expressions of TNF-alpha (P </= 0.012), IL-1beta (P </= 0.017), ICAM-1 (P </= 0.025), and IL-6 (P = 0.012), and diabetes induced increased expression of caspase-1 (P </= 0.046), VCAM-1 (P </= 0.027), ICAM-1 (P </= 0.016), IL-1beta (P = 0.016), and IL-6 (P = 0.041). Ischemia plus diabetes did not increase these findings significantly. DHA and pravastatin had some inhibitory effects in diabetic rats (P </= 0.037). CONCLUSIONS:: Inflammation triggered by ischemia-reperfusion may play a role in diabetic retinopathy. Intervention on lipid-based structures by omega-3-polyunsaturated fatty acids or statins seemed to have beneficial effects on inflammation in diabetes. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Retina
volume
28
issue
4
pages
645 - 652
publisher
Lippincott Williams & Wilkins
external identifiers
  • WOS:000255370800017
  • PMID:18398369
  • Scopus:42549101711
ISSN
0275-004X
DOI
10.1097/IAE.0b013e31815ec32d
language
English
LU publication?
yes
id
75fb11cd-9b79-4275-9d9c-4adabc269474 (old id 1147607)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18398369?dopt=Abstract
date added to LUP
2008-05-09 09:14:07
date last changed
2016-11-13 04:27:32
@misc{75fb11cd-9b79-4275-9d9c-4adabc269474,
  abstract     = {PURPOSE:: To examine the retinal inflammatory response to ischemia-reperfusion in nondiabetic and diabetic rats injected with either an omega-3-polyunsaturated fatty acid (docosahexaenoic acid [DHA]) or a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor (pravastatin). METHODS:: Diabetes was induced by an intraperitoneal injection of streptozocin, and retinal ischemia was induced by ligation of the optic nerve and vessels, followed by reperfusion for 1 hour or 24 hours. Five minutes before surgery, an intravenous injection of DHA, pravastatin, or vehicle (ethanol) was administered. The mRNA expressions of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, caspase-1, IL-1beta, P-selectin, vascular cellular adhesion molecule (VCAM)-1, and intercellular adhesion molecule (ICAM)-1 were compared between ischemic and nonischemic retinas as well as diabetic and nondiabetic nonischemic retinas. RESULTS:: Ischemia induced increased expressions of TNF-alpha (P &lt;/= 0.012), IL-1beta (P &lt;/= 0.017), ICAM-1 (P &lt;/= 0.025), and IL-6 (P = 0.012), and diabetes induced increased expression of caspase-1 (P &lt;/= 0.046), VCAM-1 (P &lt;/= 0.027), ICAM-1 (P &lt;/= 0.016), IL-1beta (P = 0.016), and IL-6 (P = 0.041). Ischemia plus diabetes did not increase these findings significantly. DHA and pravastatin had some inhibitory effects in diabetic rats (P &lt;/= 0.037). CONCLUSIONS:: Inflammation triggered by ischemia-reperfusion may play a role in diabetic retinopathy. Intervention on lipid-based structures by omega-3-polyunsaturated fatty acids or statins seemed to have beneficial effects on inflammation in diabetes.},
  author       = {Gustavsson, Carin and Agardh, Carl-David and Hagert, Per and Agardh, Elisabet},
  issn         = {0275-004X},
  language     = {eng},
  number       = {4},
  pages        = {645--652},
  publisher    = {ARRAY(0x9f20308)},
  series       = {Retina},
  title        = {Inflammatory markers in nondiabetic and diabetic rat retinas exposed to ischemia followed by reperfusion.},
  url          = {http://dx.doi.org/10.1097/IAE.0b013e31815ec32d},
  volume       = {28},
  year         = {2008},
}