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Elastin- and Collagen-Rich Human Carotid Plaques Have Increased Levels of the Cysteine Protease Inhibitor Cystatin C.

Goncalves, Isabel LU ; Ares, Mikko P S; Moberg, Anna; Moses, Jonatan LU ; To, Fong LU ; Montan, Jonathan LU ; Pedro, Luís M; Dias, Nuno LU ; Fernandes E Fernandes, José and Nordin Fredrikson, Gunilla LU , et al. (2008) In Journal of Vascular Research 45(5). p.395-401
Abstract
Background: Cystatin C is a major inhibitor of the elastin- and collagen-degrading cysteine proteases and may therefore have an important role in preserving atherosclerotic plaque stability. In this study we analyzed the associations between human carotid plaque cystatin C expression and the plaque content of collagen and elastin. Methods: Thirty-one plaques were removed by endarterectomy and homogenized. Cystatin C levels were analyzed by densitometry of Western blots and elastin and collagen levels were determined colorimetrically. Results: The plaque content of cystatin C correlated with total elastin (r = 0.58, p = 0.001) and collagen (r = 0.50, p = 0.004), as well as with cross-linked forms of elastin (r = 0.42, p = 0.022) and... (More)
Background: Cystatin C is a major inhibitor of the elastin- and collagen-degrading cysteine proteases and may therefore have an important role in preserving atherosclerotic plaque stability. In this study we analyzed the associations between human carotid plaque cystatin C expression and the plaque content of collagen and elastin. Methods: Thirty-one plaques were removed by endarterectomy and homogenized. Cystatin C levels were analyzed by densitometry of Western blots and elastin and collagen levels were determined colorimetrically. Results: The plaque content of cystatin C correlated with total elastin (r = 0.58, p = 0.001) and collagen (r = 0.50, p = 0.004), as well as with cross-linked forms of elastin (r = 0.42, p = 0.022) and collagen (r = 0.52, p = 0.003). Immunohistochemical analysis demonstrated that cystatin C colocalized with elastin and collagen. No correlation was seen between cystatin C and the amount of degraded elastin or collagen in plaques. Conclusion: The positive correlation between cystatin C levels and collagen and elastin levels in plaques supports the notion that cystatin C plays an important role in maintaining atherosclerotic plaque stability. Copyright (c) 2008 S. Karger AG, Basel. (Less)
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published
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Journal of Vascular Research
volume
45
issue
5
pages
395 - 401
publisher
Karger
external identifiers
  • WOS:000258318300004
  • PMID:18376131
  • Scopus:50349083364
ISSN
1423-0135
DOI
10.1159/000121474
language
English
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yes
id
44379c97-c874-4795-858e-764af0bbd20a (old id 1147944)
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http://www.ncbi.nlm.nih.gov/pubmed/18376131?dopt=Abstract
date added to LUP
2008-05-09 08:49:27
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2016-10-13 04:31:32
@misc{44379c97-c874-4795-858e-764af0bbd20a,
  abstract     = {Background: Cystatin C is a major inhibitor of the elastin- and collagen-degrading cysteine proteases and may therefore have an important role in preserving atherosclerotic plaque stability. In this study we analyzed the associations between human carotid plaque cystatin C expression and the plaque content of collagen and elastin. Methods: Thirty-one plaques were removed by endarterectomy and homogenized. Cystatin C levels were analyzed by densitometry of Western blots and elastin and collagen levels were determined colorimetrically. Results: The plaque content of cystatin C correlated with total elastin (r = 0.58, p = 0.001) and collagen (r = 0.50, p = 0.004), as well as with cross-linked forms of elastin (r = 0.42, p = 0.022) and collagen (r = 0.52, p = 0.003). Immunohistochemical analysis demonstrated that cystatin C colocalized with elastin and collagen. No correlation was seen between cystatin C and the amount of degraded elastin or collagen in plaques. Conclusion: The positive correlation between cystatin C levels and collagen and elastin levels in plaques supports the notion that cystatin C plays an important role in maintaining atherosclerotic plaque stability. Copyright (c) 2008 S. Karger AG, Basel.},
  author       = {Goncalves, Isabel and Ares, Mikko P S and Moberg, Anna and Moses, Jonatan and To, Fong and Montan, Jonathan and Pedro, Luís M and Dias, Nuno and Fernandes E Fernandes, José and Nordin Fredrikson, Gunilla and Nilsson, Jan and Jovinge, Stefan and Bengtsson, Eva},
  issn         = {1423-0135},
  language     = {eng},
  number       = {5},
  pages        = {395--401},
  publisher    = {ARRAY(0xb5c4808)},
  series       = {Journal of Vascular Research},
  title        = {Elastin- and Collagen-Rich Human Carotid Plaques Have Increased Levels of the Cysteine Protease Inhibitor Cystatin C.},
  url          = {http://dx.doi.org/10.1159/000121474},
  volume       = {45},
  year         = {2008},
}