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Galectin binding proteins in serum and bronchoalveolar lavage -in healthy and pathological conditions

Cederfur, Cecilia LU (2008) In Lund University, Faculty of Medicine Doctoral Dissertation Series 2008:68.
Abstract
Galectins are carbohydrate binding proteins, implicated in conditions of both inflammation and cancer. Connections between chronic inflammation and cancer are proposed by the increased remodelling and proliferation that occurs, leading to enhanced survival and proliferation of malignant cells. Since galectins have been implicated in mechanisms of both chronic inflammation and cancer, we have investigated natural binding partners of galectins in healthy individuals and then continued with studying states of cancer and chronic inflammation. We identified galectin binding glycoproteins in sera from healthy individuals and found that galectins widely expressed in the body bind serum glycoproteins well, whereas galectins with a more... (More)
Galectins are carbohydrate binding proteins, implicated in conditions of both inflammation and cancer. Connections between chronic inflammation and cancer are proposed by the increased remodelling and proliferation that occurs, leading to enhanced survival and proliferation of malignant cells. Since galectins have been implicated in mechanisms of both chronic inflammation and cancer, we have investigated natural binding partners of galectins in healthy individuals and then continued with studying states of cancer and chronic inflammation. We identified galectin binding glycoproteins in sera from healthy individuals and found that galectins widely expressed in the body bind serum glycoproteins well, whereas galectins with a more tissue-specific distribution scarcely binds any serum glycoproteins. We then chose the widely expressed but intermediately binding galectin-1 to detect if levels of galectin-1-binding proteins are increased in sera of breast cancer patients. We found that galectin-1 binds approximately double the amount in breast cancer patients compared to healthy individuals. The increase was mainly caused by haptoglobin, probably due to both increased expression and changes of glycosylation.

To further investigate the inflammatory connection we identified galectin binding proteins from bronchoalveolar lavage of asthma patients and healthy individuals, additionally we compared the binding of galectin-3 and galectin-8 that are expressed in different sites of the lung. We found when functionally grouping the bound proteins that galectin-3 and -8 binding proteins had different profiles and that bound proteins of healthy and asthma patients differed. (Less)
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author
supervisor
opponent
  • professor Påhlsson, Peter, Linköpings Universitet
organization
publishing date
type
Thesis
publication status
published
subject
keywords
cancer, glycoprotein, asthma, bronchoalveolar lavage, galectin binding protein, galectin ligand, galectin, serum
in
Lund University, Faculty of Medicine Doctoral Dissertation Series
volume
2008:68
pages
73 pages
publisher
Department of Laboratory Medicine, Lund University
defense location
Segerfalksalen, Wallenberg neurocentrum, BMC, Sölvegatan 17
defense date
2008-05-31 09:00
ISSN
1652-8220
ISBN
978-91-86059-21-7
language
English
LU publication?
yes
id
cd3a8ae4-b129-470d-961f-e0b9ed81006f (old id 1148923)
date added to LUP
2008-05-09 11:22:34
date last changed
2016-04-15 21:10:28
@misc{cd3a8ae4-b129-470d-961f-e0b9ed81006f,
  abstract     = {Galectins are carbohydrate binding proteins, implicated in conditions of both inflammation and cancer. Connections between chronic inflammation and cancer are proposed by the increased remodelling and proliferation that occurs, leading to enhanced survival and proliferation of malignant cells. Since galectins have been implicated in mechanisms of both chronic inflammation and cancer, we have investigated natural binding partners of galectins in healthy individuals and then continued with studying states of cancer and chronic inflammation. We identified galectin binding glycoproteins in sera from healthy individuals and found that galectins widely expressed in the body bind serum glycoproteins well, whereas galectins with a more tissue-specific distribution scarcely binds any serum glycoproteins. We then chose the widely expressed but intermediately binding galectin-1 to detect if levels of galectin-1-binding proteins are increased in sera of breast cancer patients. We found that galectin-1 binds approximately double the amount in breast cancer patients compared to healthy individuals. The increase was mainly caused by haptoglobin, probably due to both increased expression and changes of glycosylation. <br/><br>
To further investigate the inflammatory connection we identified galectin binding proteins from bronchoalveolar lavage of asthma patients and healthy individuals, additionally we compared the binding of galectin-3 and galectin-8 that are expressed in different sites of the lung. We found when functionally grouping the bound proteins that galectin-3 and -8 binding proteins had different profiles and that bound proteins of healthy and asthma patients differed.},
  author       = {Cederfur, Cecilia},
  isbn         = {978-91-86059-21-7},
  issn         = {1652-8220},
  keyword      = {cancer,glycoprotein,asthma,bronchoalveolar lavage,galectin binding protein,galectin ligand,galectin,serum},
  language     = {eng},
  pages        = {73},
  publisher    = {ARRAY(0x93878e0)},
  series       = {Lund University, Faculty of Medicine Doctoral Dissertation Series},
  title        = {Galectin binding proteins in serum and bronchoalveolar lavage -in healthy and pathological conditions},
  volume       = {2008:68},
  year         = {2008},
}