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Protein-based capacitive biosensors for the detection of heavy-metal ions

Mortari, Alessia LU (2008)
Abstract
Some metal ions, such as copper and zinc, are essential nutrients and catalysts in biochemical reactions. Other metal ions, e.g. cadmium and mercury, are highly toxic elements.

Heavy metals detection has proven difficult with classical as well as experimental analytical methods. Novel techniques are required for the measurement of bioavailable toxic elements and for detecting small ligands binding, often weak and transient, yet vital to most cellular processes.

The here-discussed biosensors were developed for the measurement of bioavailable concentration of toxic metals and to investigate the biochemical characteristics of proteins of biomedical interest.

Heavy metal ions binding proteins, e.g. SmtA and... (More)
Some metal ions, such as copper and zinc, are essential nutrients and catalysts in biochemical reactions. Other metal ions, e.g. cadmium and mercury, are highly toxic elements.

Heavy metals detection has proven difficult with classical as well as experimental analytical methods. Novel techniques are required for the measurement of bioavailable toxic elements and for detecting small ligands binding, often weak and transient, yet vital to most cellular processes.

The here-discussed biosensors were developed for the measurement of bioavailable concentration of toxic metals and to investigate the biochemical characteristics of proteins of biomedical interest.

Heavy metal ions binding proteins, e.g. SmtA and S100A12, were used as bio-recognition element.

Electrochemical capacitance was used to measure the protein-heavy metal ion interaction.

The aim of the thesis is to review the research and the state-of-the-art of the protein-based capacitive biosensors for the detection of heavy metal ions. It covers the main aspects of the biosensor theory, research and development, including the detection principle, with particular attention to the transducer methods and gold electrodes, heavy metal ions coordinating proteins, immobilisation methods and the experimental biosensors applications. (Less)
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author
supervisor
opponent
  • Professor Schuhmann, Wolfgang, Department of Analytical Chemistry, Ruhr-Universität Bochum, Bochum, Germany
organization
publishing date
type
Thesis
publication status
published
subject
keywords
heavy metal ions, protein, electrode, Biosensor, capacitance
publisher
Department of Analytical Chemistry, Lund University
defense location
hörsal B, Kemicentrum, Getingevägen 60
defense date
2008-09-05 10:30
ISBN
978-91-7422-201-2
language
English
LU publication?
yes
id
fed9f69f-4ab3-4194-a42f-c6b4eed0bed4 (old id 1211401)
date added to LUP
2008-08-08 09:15:50
date last changed
2016-09-19 08:45:14
@misc{fed9f69f-4ab3-4194-a42f-c6b4eed0bed4,
  abstract     = {Some metal ions, such as copper and zinc, are essential nutrients and catalysts in biochemical reactions. Other metal ions, e.g. cadmium and mercury, are highly toxic elements.<br/><br>
Heavy metals detection has proven difficult with classical as well as experimental analytical methods. Novel techniques are required for the measurement of bioavailable toxic elements and for detecting small ligands binding, often weak and transient, yet vital to most cellular processes. <br/><br>
The here-discussed biosensors were developed for the measurement of bioavailable concentration of toxic metals and to investigate the biochemical characteristics of proteins of biomedical interest. <br/><br>
Heavy metal ions binding proteins, e.g. SmtA and S100A12, were used as bio-recognition element. <br/><br>
Electrochemical capacitance was used to measure the protein-heavy metal ion interaction.<br/><br>
The aim of the thesis is to review the research and the state-of-the-art of the protein-based capacitive biosensors for the detection of heavy metal ions. It covers the main aspects of the biosensor theory, research and development, including the detection principle, with particular attention to the transducer methods and gold electrodes, heavy metal ions coordinating proteins, immobilisation methods and the experimental biosensors applications.},
  author       = {Mortari, Alessia},
  isbn         = {978-91-7422-201-2},
  keyword      = {heavy metal ions,protein,electrode,Biosensor,capacitance},
  language     = {eng},
  publisher    = {ARRAY(0x9141930)},
  title        = {Protein-based capacitive biosensors for the detection of heavy-metal ions},
  year         = {2008},
}