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RIFABUTIN ACCUMULATES IN THE LENS AND REDUCES RETINAL FUNCTION IN THE RABBIT EYE.

Cardiakidis Myers, Anna LU ; Kjellström, Sten LU ; Bruun, Anitha LU ; Isaksson, Björn LU ; Ghosh, Fredrik LU ; Andréasson, Sten LU and Ponjavic, Vesna LU (2009) In Retina 29. p.106-111
Abstract
PURPOSE:: To study the toxicology of rifabutin in the rabbit eye with emphasis on retinal function and histopathology. METHODS:: Seven rabbits received a daily dose of rifabutin during 15 months. Six rabbits receiving only the vehicle were used as controls. Repeated standardized full-field electroretinograms (ERG) were assessed. After discontinuing treatment, the rabbits were killed and the cornea, the lens, and the sectioned retina was studied. Immunhistochemistry directed against vimentin, glial fibrillaryacidic protein (GFAP), protein kinase C (PKC), and peanut agglutinin (PNA) was performed. RESULTS:: Rifabutin was detected in serum of the treated rabbits. During treatment, the full-field ERG demonstrated significantly reduced b-wave... (More)
PURPOSE:: To study the toxicology of rifabutin in the rabbit eye with emphasis on retinal function and histopathology. METHODS:: Seven rabbits received a daily dose of rifabutin during 15 months. Six rabbits receiving only the vehicle were used as controls. Repeated standardized full-field electroretinograms (ERG) were assessed. After discontinuing treatment, the rabbits were killed and the cornea, the lens, and the sectioned retina was studied. Immunhistochemistry directed against vimentin, glial fibrillaryacidic protein (GFAP), protein kinase C (PKC), and peanut agglutinin (PNA) was performed. RESULTS:: Rifabutin was detected in serum of the treated rabbits. During treatment, the full-field ERG demonstrated significantly reduced b-wave amplitudes in the total rod-cone response as well as in the isolated rod and cone response compared with the recordings before treatment. The control rabbits did not demonstrate a reduction of the ERG amplitudes. The treated rabbits developed a discoloration of the lens, not seen in the control group. No retinal pathology was demonstrated using immunohistochemical methods. CONCLUSION:: Rifabutin causes a discoloration of the lens and reduces both rod and cone function in rabbits, but does not alter retinal morphology. Previous reports on ocular side effects caused by rifabutin are supported by the results of this study. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Retina
volume
29
pages
106 - 111
publisher
Lippincott Williams & Wilkins
external identifiers
  • WOS:000262413300017
  • PMID:18685541
  • Scopus:61549105919
ISSN
0275-004X
DOI
10.1097/IAE.0b013e318180ab3c
language
English
LU publication?
yes
id
0d5b4156-1509-4bf7-8d8d-b53de0ff3c1e (old id 1223423)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18685541?dopt=Abstract
date added to LUP
2008-09-11 16:16:15
date last changed
2016-10-13 04:35:54
@misc{0d5b4156-1509-4bf7-8d8d-b53de0ff3c1e,
  abstract     = {PURPOSE:: To study the toxicology of rifabutin in the rabbit eye with emphasis on retinal function and histopathology. METHODS:: Seven rabbits received a daily dose of rifabutin during 15 months. Six rabbits receiving only the vehicle were used as controls. Repeated standardized full-field electroretinograms (ERG) were assessed. After discontinuing treatment, the rabbits were killed and the cornea, the lens, and the sectioned retina was studied. Immunhistochemistry directed against vimentin, glial fibrillaryacidic protein (GFAP), protein kinase C (PKC), and peanut agglutinin (PNA) was performed. RESULTS:: Rifabutin was detected in serum of the treated rabbits. During treatment, the full-field ERG demonstrated significantly reduced b-wave amplitudes in the total rod-cone response as well as in the isolated rod and cone response compared with the recordings before treatment. The control rabbits did not demonstrate a reduction of the ERG amplitudes. The treated rabbits developed a discoloration of the lens, not seen in the control group. No retinal pathology was demonstrated using immunohistochemical methods. CONCLUSION:: Rifabutin causes a discoloration of the lens and reduces both rod and cone function in rabbits, but does not alter retinal morphology. Previous reports on ocular side effects caused by rifabutin are supported by the results of this study.},
  author       = {Cardiakidis Myers, Anna and Kjellström, Sten and Bruun, Anitha and Isaksson, Björn and Ghosh, Fredrik and Andréasson, Sten and Ponjavic, Vesna},
  issn         = {0275-004X},
  language     = {eng},
  pages        = {106--111},
  publisher    = {ARRAY(0x7c36de8)},
  series       = {Retina},
  title        = {RIFABUTIN ACCUMULATES IN THE LENS AND REDUCES RETINAL FUNCTION IN THE RABBIT EYE.},
  url          = {http://dx.doi.org/10.1097/IAE.0b013e318180ab3c},
  volume       = {29},
  year         = {2009},
}