The gonadotropin receptors FSH-R and LH-R of Atlantic halibut (Hippoglossus hippoglossus)—2. Differential follicle expression and asynchronous oogenesis
(2008) In General and Comparative Endocrinology 156(3). p.595-602- Abstract
- The biological activity and spatio–temporal expression patterns of the gonadotropin receptors FSH-R and LH-R were examined in the repetitive spawner Atlantic halibut to elucidate the gonadotropic regulation of the asynchronous follicle development. The cloned receptors were expressed in mammalian COS-7 cells, and stimulation with sea bass FSH and LH increased the cAMP production. The halibut FSH-R and LH-R genes were shown to be highly expressed in the gonads of sexually mature fish, but the transcripts were also found in extra-gonadal tissues such as pituitary and brain. Different expression patterns of FSH-R and LH-R in the developing follicles were documented by semi-quantitative RT-PCR. Abundant FSH-R mRNA was found in the small... (More)
- The biological activity and spatio–temporal expression patterns of the gonadotropin receptors FSH-R and LH-R were examined in the repetitive spawner Atlantic halibut to elucidate the gonadotropic regulation of the asynchronous follicle development. The cloned receptors were expressed in mammalian COS-7 cells, and stimulation with sea bass FSH and LH increased the cAMP production. The halibut FSH-R and LH-R genes were shown to be highly expressed in the gonads of sexually mature fish, but the transcripts were also found in extra-gonadal tissues such as pituitary and brain. Different expression patterns of FSH-R and LH-R in the developing follicles were documented by semi-quantitative RT-PCR. Abundant FSH-R mRNA was found in the small follicles during primary growth and vitellogenesis, and the signals were localized to the granulosa cells by in situ hybridization. In contrast, follicular LH-R mRNA was hardly detectable during the early stages. Conversely, in follicles during final maturation FSH-R mRNA levels tended to decrease, while the expression of LH-R was highly upregulated. Whereas the pituitary FSH and LH are asynchronously expressed in annual spawners, both gonadotropins were expressed in the female halibut pituitary throughout the reproductive cycle, except in the prespawning females. Hence, the sequential gonadotropic activation of ovarian follicle growth and maturation in repetitive spawners is probably regulated by modulating the temporal expression of FSH-R and LH-R in the follicle membrane. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1245777
- author
- Kobayashi, Tamae LU ; Pakarinen, Pirjo ; Torgersen, Jacob ; Huhtaniemi, Ilpo and Andersen, Øivind
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- General and Comparative Endocrinology
- volume
- 156
- issue
- 3
- pages
- 595 - 602
- publisher
- Elsevier
- external identifiers
-
- scopus:42649097561
- ISSN
- 0016-6480
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Tumour Cell Biology (013017530)
- id
- de7d6810-683b-4679-a713-efc52faeca19 (old id 1245777)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18377904
- http://www.sciencedirect.com/science/article/pii/S0016648008000889
- date added to LUP
- 2016-04-04 13:15:57
- date last changed
- 2022-02-28 22:00:22
@article{de7d6810-683b-4679-a713-efc52faeca19, abstract = {{The biological activity and spatio–temporal expression patterns of the gonadotropin receptors FSH-R and LH-R were examined in the repetitive spawner Atlantic halibut to elucidate the gonadotropic regulation of the asynchronous follicle development. The cloned receptors were expressed in mammalian COS-7 cells, and stimulation with sea bass FSH and LH increased the cAMP production. The halibut FSH-R and LH-R genes were shown to be highly expressed in the gonads of sexually mature fish, but the transcripts were also found in extra-gonadal tissues such as pituitary and brain. Different expression patterns of FSH-R and LH-R in the developing follicles were documented by semi-quantitative RT-PCR. Abundant FSH-R mRNA was found in the small follicles during primary growth and vitellogenesis, and the signals were localized to the granulosa cells by in situ hybridization. In contrast, follicular LH-R mRNA was hardly detectable during the early stages. Conversely, in follicles during final maturation FSH-R mRNA levels tended to decrease, while the expression of LH-R was highly upregulated. Whereas the pituitary FSH and LH are asynchronously expressed in annual spawners, both gonadotropins were expressed in the female halibut pituitary throughout the reproductive cycle, except in the prespawning females. Hence, the sequential gonadotropic activation of ovarian follicle growth and maturation in repetitive spawners is probably regulated by modulating the temporal expression of FSH-R and LH-R in the follicle membrane.}}, author = {{Kobayashi, Tamae and Pakarinen, Pirjo and Torgersen, Jacob and Huhtaniemi, Ilpo and Andersen, Øivind}}, issn = {{0016-6480}}, language = {{eng}}, number = {{3}}, pages = {{595--602}}, publisher = {{Elsevier}}, series = {{General and Comparative Endocrinology}}, title = {{The gonadotropin receptors FSH-R and LH-R of Atlantic halibut (Hippoglossus hippoglossus)—2. Differential follicle expression and asynchronous oogenesis}}, url = {{http://www.ncbi.nlm.nih.gov/pubmed/18377904}}, volume = {{156}}, year = {{2008}}, }