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Identification of common traits in improved xylose-growing Saccharomyces cerevisiae for inverse metabolic engineering.

Bengtsson, Oskar LU ; Jeppsson, Marie; Sonderegger, Marco; Parachin, Nadia Skorupa; Sauer, Uwe; Hahn-Hägerdal, Bärbel and Gorwa-Grauslund, Marie-F (2008) In Yeast (Chichester, England) 25(11). p.835-847
Abstract
Four recombinant Saccharomyces cerevisiae strains with enhanced xylose growth (TMB3400, C1, C5 and BH42) were compared with two control strains (TMB3399, TMB3001) through genome-wide transcription analysis in order to identify novel targets for inverse metabolic engineering. A subset of 13 genes with changed expression levels in all improved strains was selected for further analysis. Thirteen validation strains and two reference strains were constructed to investigate the effect of overexpressing or deleting these genes in xylose-utilizing S. cerevisiae. Improved aerobic growth rates on xylose were observed in five cases. The strains overexpressing SOL3 and TAL1 grew 19% and 24% faster than their reference strain, and the strains carrying... (More)
Four recombinant Saccharomyces cerevisiae strains with enhanced xylose growth (TMB3400, C1, C5 and BH42) were compared with two control strains (TMB3399, TMB3001) through genome-wide transcription analysis in order to identify novel targets for inverse metabolic engineering. A subset of 13 genes with changed expression levels in all improved strains was selected for further analysis. Thirteen validation strains and two reference strains were constructed to investigate the effect of overexpressing or deleting these genes in xylose-utilizing S. cerevisiae. Improved aerobic growth rates on xylose were observed in five cases. The strains overexpressing SOL3 and TAL1 grew 19% and 24% faster than their reference strain, and the strains carrying deletions of YLR042C, MNI1 or RPA49 grew 173%, 62% and 90% faster than their reference strain. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Xylose: metabolism, Saccharomyces cerevisiae: metabolism, Saccharomyces cerevisiae: genetics, Saccharomyces cerevisiae: growth & development
in
Yeast (Chichester, England)
volume
25
issue
11
pages
835 - 847
publisher
John Wiley & Sons
external identifiers
  • WOS:000262033500006
  • PMID:19061191
  • Scopus:58149347653
ISSN
1097-0061
DOI
10.1002/yea.1638
language
English
LU publication?
yes
id
6cafe953-cab8-4d20-a0da-395fa261dd00 (old id 1276361)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19061191?dopt=Abstract
date added to LUP
2009-01-09 11:41:11
date last changed
2016-10-30 04:35:57
@misc{6cafe953-cab8-4d20-a0da-395fa261dd00,
  abstract     = {Four recombinant Saccharomyces cerevisiae strains with enhanced xylose growth (TMB3400, C1, C5 and BH42) were compared with two control strains (TMB3399, TMB3001) through genome-wide transcription analysis in order to identify novel targets for inverse metabolic engineering. A subset of 13 genes with changed expression levels in all improved strains was selected for further analysis. Thirteen validation strains and two reference strains were constructed to investigate the effect of overexpressing or deleting these genes in xylose-utilizing S. cerevisiae. Improved aerobic growth rates on xylose were observed in five cases. The strains overexpressing SOL3 and TAL1 grew 19% and 24% faster than their reference strain, and the strains carrying deletions of YLR042C, MNI1 or RPA49 grew 173%, 62% and 90% faster than their reference strain.},
  author       = {Bengtsson, Oskar and Jeppsson, Marie and Sonderegger, Marco and Parachin, Nadia Skorupa and Sauer, Uwe and Hahn-Hägerdal, Bärbel and Gorwa-Grauslund, Marie-F},
  issn         = {1097-0061},
  keyword      = {Xylose: metabolism,Saccharomyces cerevisiae: metabolism,Saccharomyces cerevisiae: genetics,Saccharomyces cerevisiae: growth & development},
  language     = {eng},
  number       = {11},
  pages        = {835--847},
  publisher    = {ARRAY(0xa0e7a30)},
  series       = {Yeast (Chichester, England)},
  title        = {Identification of common traits in improved xylose-growing Saccharomyces cerevisiae for inverse metabolic engineering.},
  url          = {http://dx.doi.org/10.1002/yea.1638},
  volume       = {25},
  year         = {2008},
}