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Characterization of the human CREB3L2 gene promoter

Panagopoulos, Ioannis LU and Mertens, Fredrik LU (2009) In Oncology Reports 21(3). p.615-624
Abstract

CREB3L2 encodes a member of the CREB3 family of transcription factors. We characterized its promoter region, showing that it is asymmetrically bidirectional, also driving the expression of a variant of AKR1D1. It has a CRE binding site which is conserved among mammalians; removal or alteration of it resulted in reduced promoter activity. When transiently transfecting the HEK293 cell line with constructs with partially deleted promoter regions, 5' deletions beyond 1058-bp upstream of the transcription starting site resulted in successive reduction of the activity. The inclusion of the untranslated part of CREB3L2 exon 1 strongly inhibited the promoter activity. Forskolin resulted in a decreased reporter activity, whereas phorbol... (More)

CREB3L2 encodes a member of the CREB3 family of transcription factors. We characterized its promoter region, showing that it is asymmetrically bidirectional, also driving the expression of a variant of AKR1D1. It has a CRE binding site which is conserved among mammalians; removal or alteration of it resulted in reduced promoter activity. When transiently transfecting the HEK293 cell line with constructs with partially deleted promoter regions, 5' deletions beyond 1058-bp upstream of the transcription starting site resulted in successive reduction of the activity. The inclusion of the untranslated part of CREB3L2 exon 1 strongly inhibited the promoter activity. Forskolin resulted in a decreased reporter activity, whereas phorbol 12-myristate 13-acetate increased the promoter activity irrespective of the status of the CRE binding site. The presence of the CRE site indicates autoregulation of CREB3L2 and/or regulation via other members of the CREB3 family or a variety of bZIP transcription factors.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Base Sequence, Basic-Leucine Zipper Transcription Factors, Gene Expression Regulation, Humans, Molecular Sequence Data, Mutagenesis, Site-Directed, Promoter Regions, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Nucleic Acid, Journal Article, Research Support, Non-U.S. Gov't
in
Oncology Reports
volume
21
issue
3
pages
10 pages
publisher
D.A. Spandidos
external identifiers
  • WOS:000263584000007
  • PMID:19212619
  • Scopus:64849107204
ISSN
1791-2431
DOI
10.3892/or_00000264
language
English
LU publication?
yes
id
946ee665-f664-4c15-b18f-83ebf6766f78 (old id 1302653)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19212619?dopt=Abstract
date added to LUP
2009-03-02 14:24:28
date last changed
2016-10-13 04:23:31
@misc{946ee665-f664-4c15-b18f-83ebf6766f78,
  abstract     = {<p>CREB3L2 encodes a member of the CREB3 family of transcription factors. We characterized its promoter region, showing that it is asymmetrically bidirectional, also driving the expression of a variant of AKR1D1. It has a CRE binding site which is conserved among mammalians; removal or alteration of it resulted in reduced promoter activity. When transiently transfecting the HEK293 cell line with constructs with partially deleted promoter regions, 5' deletions beyond 1058-bp upstream of the transcription starting site resulted in successive reduction of the activity. The inclusion of the untranslated part of CREB3L2 exon 1 strongly inhibited the promoter activity. Forskolin resulted in a decreased reporter activity, whereas phorbol 12-myristate 13-acetate increased the promoter activity irrespective of the status of the CRE binding site. The presence of the CRE site indicates autoregulation of CREB3L2 and/or regulation via other members of the CREB3 family or a variety of bZIP transcription factors.</p>},
  author       = {Panagopoulos, Ioannis and Mertens, Fredrik},
  issn         = {1791-2431},
  keyword      = {Base Sequence,Basic-Leucine Zipper Transcription Factors,Gene Expression Regulation,Humans,Molecular Sequence Data,Mutagenesis, Site-Directed,Promoter Regions, Genetic,Reverse Transcriptase Polymerase Chain Reaction,Sequence Homology, Nucleic Acid,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {615--624},
  publisher    = {ARRAY(0x938f498)},
  series       = {Oncology Reports},
  title        = {Characterization of the human CREB3L2 gene promoter},
  url          = {http://dx.doi.org/10.3892/or_00000264},
  volume       = {21},
  year         = {2009},
}